FlyBase Allele Report: Dmel\KdelR[GD1228]

General Information

Symbol

Dmel\KdelR^GD1228

Species

D. melanogaster

Name

FlyBase ID

FBal0207441

Feature type

allele

Associated gene

Dmel\KdelR

Associated Insertion(s)

Carried in Construct

P{GD1228}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1228}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1228

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

decreased cell number | larval stage, with Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Zhang et al., 2024)

lethal, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

partially lethal, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

short lived, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

Phenotype Manifest In

type II neuroblast | decreased number | larval stage, with Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Zhang et al., 2024)

Detailed Description

Statement

Reference

The number of lamellocytes in hemocyte smears from third instar larvae expressing KdelR^GD1228 under the control of Scer\GAL4^Hml.Δ is unchanged (insertion v9235) or decreased (insertion v9236) and relative to wild-type, adult flies show more extensive eye degeneration after exposure of one eye to UV irradiation during pupal stage.

(Neves et al., 2016)

Adults expressing KdelR^GD1228 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

(Neely et al., 2010)

Adults expressing KdelR^GD1228 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) can show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies, depending on the particular P{GD1228} insertion line used.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in lethality by 7 days after eclosion.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality before the pupal stage.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1228

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

KdelR^GD1228, Scer\GAL4^Hml.Δ is a suppressor of lamellocyte | increased number | third instar larval stage phenotype of Manf^UAS.cPa, Scer\GAL4^Hml.Δ

(Neves et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of lamellocytes in hemocyte smears from third instar larvae as well as the adult eye tissue repair defects characteristic for flies expressing Manf^Scer\UAS.cPa under the control of Scer\GAL4^Hml.Δ is suppressed by co-expression of KdelR^GD1228.

(Neves et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v9235