FlyBase Allele Report: Dmel\Manf[UAS.cPa]

General Information

Symbol

Dmel\Manf^UAS.cPa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Palgi

FlyBase ID

FBal0221495

Feature type

allele

Associated gene

Dmel\Manf

Associated Insertion(s)

Carried in Construct

P{UAS-Manf.P}

Also Known As

UAS-DmManf

Key Links

Transgenic product class

cDNA

(Palgi et al., 2009)

wild_type

(Palgi et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Palgi et al., 2009)

wild_type

(Palgi et al., 2009)

Mutagen

in vitro construct

(Palgi et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-Manf.P}

Cytology

Description

UASt regulatory sequences drive expression of a Manf cDNA.

(Palgi et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Manf

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye | adult stage, with Scer\GAL4^Hml.Δ

(Neves et al., 2016)

lamellocyte | increased number | third instar larval stage, with Scer\GAL4^Hml.Δ

(Neves et al., 2016)

lamina | adult stage, with Scer\GAL4^repo.PU

(Walkowicz et al., 2021)

LNv neuron | adult stage, with Scer\GAL4^Eaat1.PU

(Walkowicz et al., 2021)

Detailed Description

Statement

Reference

Adult flies expressing Manf^Scer\UAS.cPa under the control of Scer\GAL4^Hml.Δ show improved eye tissue regeneration capacity upon UV radiation induced damage (inflicted during pupal stage) compared to wild-type.

The number of lamellocytes in hemocyte smears from third instar larvae expressing Manf^Scer\UAS.cPa under the control of Scer\GAL4^Hml.Δ is significantly increased compared to wild-type.

(Neves et al., 2016)

Expression of Manf^UAS.cPa under the control of Scer\GAL4^elav.PLu, Scer\GAL4^repo or Scer\GAL4^ple.PF does not affect the number of dopaminergic neurons in any studied cluster (PAL, PPL1, PPL2ab, PMM1/2 or PPM3) of the adult brain; these animals also develop normally and live for at least 30 days.

(Stratoulias and Heino, 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Manf^UAS.cPa, Scer\GAL4^Hml.Δ is a suppressor of abnormal stress response | adult stage phenotype of Pvr^KK101575, Scer\GAL4^Hml.Δ

(Neves et al., 2016)

Manf^UAS.cPa, Scer\GAL4^Hml.Δ is a suppressor of visible | adult stage phenotype of Pvr^KK101575, Scer\GAL4^Hml.Δ

(Neves et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Manf^UAS.cPa, Scer\GAL4^Hml.Δ has lamellocyte | increased number | third instar larval stage phenotype, suppressible by KdelR^GD1228, Scer\GAL4^Hml.Δ

(Neves et al., 2016)

Suppressor of

Statement

Reference

Manf^UAS.cPa, Scer\GAL4^Hml.Δ is a suppressor of eye | adult stage phenotype of Pvr^KK101575, Scer\GAL4^Hml.Δ

(Neves et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of lamellocytes in hemocyte smears from third instar larvae as well as the adult eye tissue repair defects characteristic for flies expressing Manf^Scer\UAS.cPa under the control of Scer\GAL4^Hml.Δ is suppressed by co-expression of KdelR^GD1228.

The increased level of eye tissue degeneration upon UV irradiation (administered during pupal stage) characteristic for adult flies expressing Pvr^KK101575 under the control of Scer\GAL4^Hml.Δ can be rescued by co-expression of Manf^Scer\UAS.cPa.

Expression of Manf^Scer\UAS.cPa under the control of Scer\GAL4^Hml.Δ reduces the severity of the retinal degeneration phenotype characteristic for adult flies expressing either grim^GMR.PC or ninaE^G69D.GMR.

(Neves et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^Hml.Δ/Manf^UAS.cPa rescues Manf[+]/Manf^Δ96

(Neves et al., 2016)

Scer\GAL4^Hml.Δ/Manf^UAS.cPa rescues Manf[+]/Manf^Δ112

(Neves et al., 2016)

Scer\GAL4^da.G32/Manf^UAS.cPa rescues Manf^Δ96

(Lindström et al., 2013)

Manf^UAS.cPa/Scer\GAL4^69B rescues Manf^Δ96

(Lindström et al., 2013)

Scer\GAL4^da.G32/Manf^UAS.cPa rescues Manf^Δ96

(Palgi et al., 2009)

Scer\GAL4^Tub.PU/Manf^UAS.cPa rescues Manf^Δ96

(Palgi et al., 2009)

Manf^UAS.cPa/Scer\GAL4^69B rescues Manf^Δ96

(Palgi et al., 2009)

Fails to rescue

Manf^UAS.cPa/Scer\GAL4^path-c135 fails to rescue Manf^Δ96

(Palgi et al., 2009)

Comments

The increased severity of eye degeneration after exposure of one eye to UV irradiation during pupal stage characteristic for Manf^Δ96/+ or Manf^Δ112/+ adults can be rescued by expression of Manf^Scer\UAS.cPa under the control of Scer\GAL4^Hml.Δ.

(Neves et al., 2016)

Manf^Scer\UAS.cPa expression driven by Scer\GAL4^da.G32 rescues the early larval lethality of Manf^Δ96 mutants and normalizes Manf^Δ96 larval dopamine to wild type levels.

Manf^Scer\UAS.cPa expression driven by Scer\GAL4^69B rescues the early larval lethality of Manf^Δ96 mutants.

(Lindström et al., 2013)

Expression of Manf^Scer\UAS.cPa under the control of one of Scer\GAL4^da.G32, Scer\GAL4^tub or Scer\GAL4^69B rescues the larval lethality of Manf^Δ96 animals (assayed by survival into the pupal stage).

Expression of Manf^Scer\UAS.cPa under the control of Scer\GAL4^69B rescues the embryonic lethality of Manf^Δ96 embryos that lack both maternal and zygotic Manf[+] function.

(Palgi et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Manf^Scer\UAS.cPa

Manf^UAS.cPa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Palgi

Secondary FlyBase IDs

References (7)

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