FlyBase Allele Report: Dmel\Mgtor[GD13871]

General Information

Symbol

Dmel\Mgtor^GD13871

Species

D. melanogaster

Name

FlyBase ID

FBal0207676

Feature type

allele

Associated gene

Dmel\Mgtor

Associated Insertion(s)

Carried in Construct

P{GD13871}

Also Known As

Mtor RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD13871}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD13871

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Mtor^GD13871 driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16} results in decreased numbers of germline stem cells in the adult germinal proliferation center, which become detached from the hub, as compared to controls; concomitantly, cyst somatic stem cells become associated with the hub.

Expression of Mtor^GD13871 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} (in combination with Gal80[ts] to restrict expression to adulthood) results in mitotic germline stem cells presenting increased frequency of misoriented centrosomes, increased frequency of a range mitotic spindle defects including misorientation, and increased frequency of chromosome segregation defects, as compared to controls.

(Liu et al., 2015)

Adults expressing Mtor^GD13871 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in adult/pharate lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 100% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 100% of the Scer\GAL4^pnr-MD237 expression domain.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

13871

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Mgtor^GD13871, Scer\GAL4^{VP16.nanos.UTR} has decreased cell number | adult stage phenotype, suppressible by mad2^UAS.cLa, Scer\GAL4^{VP16.nanos.UTR}

(Liu et al., 2015)

NOT suppressed by

Statement

Reference

Mgtor^GD13871, Scer\GAL4^{VP16.nanos.UTR} has decreased cell number | adult stage phenotype, non-suppressible by Apc2^UAS.cUa, Scer\GAL4^{VP16.nanos.UTR}

(Liu et al., 2015)

Mgtor^GD13871, Scer\GAL4^{VP16.nanos.UTR} has decreased cell number | adult stage phenotype, non-suppressible by shg^UAS.sgGFP, Scer\GAL4^{VP16.nanos.UTR}

(Liu et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Mgtor^GD13871, Scer\GAL4^{VP16.nanos.UTR} has testis | adult stage phenotype, suppressible by mad2^UAS.cLa, Scer\GAL4^{VP16.nanos.UTR}

(Liu et al., 2015)

Mgtor^GD13871, Scer\GAL4^{VP16.nanos.UTR} has male germline stem cell | adult stage phenotype, suppressible by mad2^UAS.cLa, Scer\GAL4^{VP16.nanos.UTR}

(Liu et al., 2015)

NOT suppressed by

Statement

Reference

Mgtor^GD13871, Scer\GAL4^{VP16.nanos.UTR} has testis | adult stage phenotype, non-suppressible by Apc2^UAS.cUa, Scer\GAL4^{VP16.nanos.UTR}

(Liu et al., 2015)

Mgtor^GD13871, Scer\GAL4^{VP16.nanos.UTR} has male germline stem cell | adult stage phenotype, non-suppressible by Apc2^UAS.cUa, Scer\GAL4^{VP16.nanos.UTR}

(Liu et al., 2015)

Mgtor^GD13871, Scer\GAL4^{VP16.nanos.UTR} has testis | adult stage phenotype, non-suppressible by shg^UAS.sgGFP, Scer\GAL4^{VP16.nanos.UTR}

(Liu et al., 2015)

Mgtor^GD13871, Scer\GAL4^{VP16.nanos.UTR} has male germline stem cell | adult stage phenotype, non-suppressible by shg^UAS.sgGFP, Scer\GAL4^{VP16.nanos.UTR}

(Liu et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of mad2^Scer\UAS.cLa significantly rescues the decreased germline stem cell number defects present in the testis of adults expressing Mtor^GD13871 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

Co-expression of either shg^{Scer\UAS.T:Avic\GFP-rs} or Apc2^Scer\UAS.cUa does not suppress the decreased germline stem cell number defects present in the testis of adults expressing Mtor^GD13871 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Liu et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Mgtor^GD13871 is rescued by Scer\GAL4^{VP16.nanos.UTR}/Mgtor^UAS.cLa

(Liu et al., 2015)

Comments

Co-expression of Mtor^Scer\UAS.cLa under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues the decreased germline stem cell numbers present in the testis of adults expressing Mtor^GD13871 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}, as compared to controls.

(Liu et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v24265

w¹¹¹⁸; P{GD13871}v24265

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

13871

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Mgtor^GD13871

Mtor^GD13871

Mtor^RNAi-3

(Liu et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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