FlyBase Allele Report: Dmel\ND-42[GD6220]

General Information

Symbol

Dmel\ND-42^GD6220

Species

D. melanogaster

Name

FlyBase ID

FBal0207697

Feature type

allele

Associated gene

Dmel\ND-42

Associated Insertion(s)

Carried in Construct

P{GD6220}

Also Known As

ND42^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD6220}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD6220

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates cancer

modeled by Wwox^KK108459

(Choo et al., 2015)

modeled by Wwox^GD12175

(Choo et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

abnormal neuroanatomy | larval stage, with Scer\GAL4^ey.PU

(Choo et al., 2015)

abnormal neuroanatomy | progressive, with Scer\GAL4^GMR.PU

(Zhang et al., 2013)

increased occurrence of cell division | larval stage, with Scer\GAL4^ey.PU

(Choo et al., 2015)

lethal, with Scer\GAL4^da.PU

(Pogson et al., 2014)

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

neoplasia | larval stage, with Scer\GAL4^ey.PU

(Choo et al., 2015)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

eye disc, with Scer\GAL4^ey.PU

(Choo et al., 2015)

eye photoreceptor cell | progressive, with Scer\GAL4^GMR.PU

(Zhang et al., 2013)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

ommatidium, with Scer\GAL4^ey.PU

(Choo et al., 2015)

pigment cell, with Scer\GAL4^GMR.PU

(Zhang et al., 2013)

rhabdomere | progressive, with Scer\GAL4^GMR.PU

(Zhang et al., 2013)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

The expression of ND-42^GD6220 under the control of Scer\GAL4^ey.PU induces disruption to the patterning of ommatidia; a few eye discs exhibit overgrowths, which comprise both differentiated (neuronal, elav-marked) and mitotic cells.

(Choo et al., 2015)

Whole-eye knockdown of ND-42, through expression of ND-42^GD6220 under the control of Scer\GAL4^ey.PH leads to lipid droplet accumulation and peroxidation. Aconitase activity is reduced to less than 50% of wild-type in these mutants and there is a strong correlation between decreased aconitase enzymatic activity and the number of lipid droplets per ommatidium, suggesting reactive oxygen species may play a role in lipid droplet accumulation.

Neuronal expression of ND-42^GD6220 (under the control of Scer\GAL4^elav.PU) causes significant lipid droplet accumulation in glia but not neurons.

Glial expression of ND-42^GD6220 (under the control of Scer\GAL4^54C) does not lead to lipid droplet accumulation.

(Liu et al., 2015)

Newly eclosed flies expressing ND42^GD6220 under the control of Scer\GAL4^GMR.PU have a normal number and arrangement of rhabdomeres, but the pigment cells are expanded compared to wild type. At 10 days of age, the rhabdomeres are missing or dissociating and the cell bodies of some photoreceptors are vacuolated.

(Zhang et al., 2013)

Expression of ND42^GD6220 under the control of Scer\GAL4^en-e16E results in increased mitochondrial, but not cytosolic, glutathione oxidation in the posterior compartment of the wing disc.

(Albrecht et al., 2011)

Adults expressing ND-42^GD6220 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in late larval lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal larval body wall muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in wild-type sarcomeres in the larval body wall muscles.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 80-90% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 90-100% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 10-20% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development