Open Close
General Information
Symbol
Dmel\ND-42GD6220
Species
D. melanogaster
Name
FlyBase ID
FBal0207697
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Whole-eye knockdown of ND-42, through expression of ND-42GD6220 under the control of Scer\GAL4ey.PH leads to lipid droplet accumulation and peroxidation. Aconitase activity is reduced to less than 50% of wild-type in these mutants and there is a strong correlation between decreased aconitase enzymatic activity and the number of lipid droplets per ommatidium, suggesting reactive oxygen species may play a role in lipid droplet accumulation.

Neuronal expression of ND-42GD6220 (under the control of Scer\GAL4elav.PU) causes significant lipid droplet accumulation in glia but not neurons.

Glial expression of ND-42GD6220 (under the control of Scer\GAL454C) does not lead to lipid droplet accumulation.

Newly eclosed flies expressing ND42GD6220 under the control of Scer\GAL4GMR.PU have a normal number and arrangement of rhabdomeres, but the pigment cells are expanded compared to wild type. At 10 days of age, the rhabdomeres are missing or dissociating and the cell bodies of some photoreceptors are vacuolated.

Expression of ND42GD6220 under the control of Scer\GAL4en-e16E results in increased mitochondrial, but not cytosolic, glutathione oxidation in the posterior compartment of the wing disc.

Adults expressing ND-42GD6220 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression under the control of Scer\GAL4Mef2.PR results in late larval lethality.

Expression under the control of Scer\GAL4Mef2.PR results in grossly normal larval body wall muscles.

Expression under the control of Scer\GAL4Mef2.PR results in wild-type sarcomeres in the larval body wall muscles.

Expression under the control of Scer\GAL4pnr-MD237 results in a colour difference between the central Scer\GAL4pnr-MD237 expression domain of the notum and the surrounding lateral region in 80-90% of the Scer\GAL4pnr-MD237 expression domain.

Expression under the control of Scer\GAL4pnr-MD237 results in bristle morphology defects on the notum in 90-100% of the Scer\GAL4pnr-MD237 expression domain.

Expression under the control of Scer\GAL4pnr-MD237 results in the absence of 10-20% of the Scer\GAL4pnr-MD237-expressing area of the notum.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
ND-42GD6220
ND42GD6220
Name Synonyms
Secondary FlyBase IDs
    References (9)