Whole-eye knockdown of ND-42, through expression of ND-42GD6220 under the control of Scer\GAL4ey.PH leads to lipid droplet accumulation and peroxidation. Aconitase activity is reduced to less than 50% of wild-type in these mutants and there is a strong correlation between decreased aconitase enzymatic activity and the number of lipid droplets per ommatidium, suggesting reactive oxygen species may play a role in lipid droplet accumulation.
Neuronal expression of ND-42GD6220 (under the control of Scer\GAL4elav.PU) causes significant lipid droplet accumulation in glia but not neurons.
Glial expression of ND-42GD6220 (under the control of Scer\GAL454C) does not lead to lipid droplet accumulation.
Newly eclosed flies expressing ND42GD6220 under the control of Scer\GAL4GMR.PU have a normal number and arrangement of rhabdomeres, but the pigment cells are expanded compared to wild type. At 10 days of age, the rhabdomeres are missing or dissociating and the cell bodies of some photoreceptors are vacuolated.
Expression of ND42GD6220 under the control of Scer\GAL4en-e16E results in increased mitochondrial, but not cytosolic, glutathione oxidation in the posterior compartment of the wing disc.
Adults expressing ND-42GD6220 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression under the control of Scer\GAL4Mef2.PR results in late larval lethality.
Expression under the control of Scer\GAL4Mef2.PR results in grossly normal larval body wall muscles.
Expression under the control of Scer\GAL4Mef2.PR results in wild-type sarcomeres in the larval body wall muscles.