FlyBase Allele Report: Dmel\Pxd[GD16228]

General Information

Symbol

Dmel\Pxd^GD16228

Species

D. melanogaster

Name

FlyBase ID

FBal0208186

Feature type

allele

Associated gene

Dmel\Pxd

Associated Insertion(s)

Carried in Construct

P{GD16228}

Also Known As

Pxd RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD16228}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD16228

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4^nub-AC-62

(Sabarís et al., 2025)

wing vein | ectopic, with Scer\GAL4^nub-AC-62

(Sabarís et al., 2025)

Detailed Description

Statement

Reference

Expression of Pxd^GD16228 under the control of Scer\GAL4^sal.BO (in combination with a Dicer-2 transgene to enhance RNAi efficiency) does not result in larval lethality at the L2/L3 transition.

(Wicker-Thomas et al., 2015)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in loss of neuroblasts in the larval brain associated with decreased size of the remaining neuroblasts.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in a reduction in the average size of neuroblasts together with shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

(Neumüller et al., 2011)

Adults expressing Pxd^GD16228 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 40-50% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 40-50% or 60-70% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

16228

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Scer\GAL4^ap.PU/Pxd^GD16228 is a non-suppressor of visible | dominant phenotype of Duox^K

(Hurd et al., 2015)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Scer\GAL4^ap.PU/Pxd^GD16228 is a non-suppressor of wing phenotype of Duox^K

(Hurd et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Pxd^GD16228 under the control of Scer\GAL4^ap.PU does not suppress the 'Curly' wing phenotype (upward curvature of the wing) of Cy^K heterozygotes.

(Hurd et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v48587