FlyBase Allele Report: Dmel\Rh50[GD3847]

General Information

Symbol

Dmel\Rh50^GD3847

Species

D. melanogaster

Name

FlyBase ID

FBal0208278

Feature type

allele

Associated gene

Dmel\Rh50

Associated Insertion(s)

Carried in Construct

P{GD3847}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD3847}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD3847

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body size | P-stage, with Scer\GAL4^da.G32

(Lecompte et al., 2020)

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^C57

(Lecompte et al., 2020)

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^how-24B

(Lecompte et al., 2020)

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^Mhc.PK

(Lecompte et al., 2020)

abnormal neurophysiology | third instar larval stage, with Scer\GAL4^how-24B

(Lecompte et al., 2020)

abnormal size | third instar larval stage, with Scer\GAL4^how-24B

(Lecompte et al., 2020)

lethal, with Scer\GAL4^da.G32

(Lecompte et al., 2020)

some die during larval stage, with Scer\GAL4^how-24B

(Lecompte et al., 2020)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

hypodermal muscle cell of A1-7 | third instar larval stage, with Scer\GAL4^how-24B

(Lecompte et al., 2020)

Detailed Description

Statement

Reference

Expression of Rh50^GD3847 under the control of Scer\GAL4^da.G32 (in combination with a Dicer-2 transgene to enhance RNAi efficiency) leads to lethality that occurred at different stages of development according to the temperature at which the flies are reared. Animals raised at 29[o] die before third instar larvae while the ones raised at 25[o] survive until pupal stage.

Expression of Rh50^GD3847 under the control of Scer\GAL4^da.G32 (in combination with a Dicer-2) leads to longer and thinner pupae.

Expression of Rh50^GD3847 under the control of Scer\GAL4^how-24B (in combination with a Dicer-2) leads to narrower and more elongated body wall muscles, which display an irregular shape that resemble a dystrophy, in third instar larvae.

Expression of Rh50^GD3847 under the control of Scer\GAL4^how-24B,Scer\GAL4^C57 or Scer\GAL4^Mhc.PK (in combination with a Dicer-2) leads to impaired crawling of third instar larvae.

Expression of Rh50^GD3847 under the control of Scer\GAL4^how-24B (in combination with a Dicer-2) leads to an increase in the mean frequency of miniature EPSPs (mEPSPs) in Muscles 6 and 7 of third instar larvae.

(Lecompte et al., 2020)

Adults expressing Rh50^GD3847 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development