FlyBase Allele Report: Dmel\AMPKα[GD736]

General Information

Symbol

Dmel\AMPKα^GD736

Species

D. melanogaster

Name

FlyBase ID

FBal0208455

Feature type

allele

Associated gene

Dmel\AMPKα

Associated Insertion(s)

Carried in Construct

P{GD736}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD736}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD736

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Parkinson's disease

modeled by Hsap\LRRK2^{G2019S.UAS.Tag:MYC}

(Ng et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response | adult stage, with Scer\GAL4^hs.PB

(Lee et al., 2018)

abnormal immune response | adult stage, with Scer\GAL4^NP0001

(Lee et al., 2018)

increased mortality | adult stage | conditional, with Scer\GAL4^hs.PB

(Lee et al., 2018)

increased mortality | adult stage | conditional, with Scer\GAL4^NP0001

(Lee et al., 2018)

lethal, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

adult midgut | conditional, with Scer\GAL4^NP0001

(Lee et al., 2018)

autophagosome | adult stage | conditional, with Scer\GAL4^NP0001

(Lee et al., 2018)

lipid droplet | adult stage | conditional, with Scer\GAL4^NP0001

(Lee et al., 2018)

Detailed Description

Statement

Reference

The expression of AMPKα^GD736, driven by Scer\GAL4^NP0001 or driven by Scer\GAL4^hs.PB and induced by 29[o]C, but not when driven by Scer\GAL4^ppl.PU, induces significantly higher mortality to Ecc15 enteric infection, as compared to controls; Scer\GAL4^NP0001-driven expression also abolishes the Ecc15 infection-induced lipid droplet autophagy (dual LS2- and Atg8-positive puncta) in the adult midgut observed in controls.

(Lee et al., 2018)

Adults expressing AMPKα^GD736 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in early pupal lethality or undefined lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

AMPKα^GD736, Scer\GAL4^hs.PB has increased mortality | adult stage | conditional phenotype, suppressible by Acc^KK102082, Scer\GAL4^hs.PB

(Lee et al., 2018)

AMPKα^GD736, Scer\GAL4^hs.PB has abnormal immune response | adult stage phenotype, suppressible by Acc^KK102082, Scer\GAL4^hs.PB

(Lee et al., 2018)

AMPKα^GD736, Scer\GAL4^hs.PB has increased mortality | adult stage | conditional phenotype, suppressible by bmm^UAS.cUa, Scer\GAL4^hs.PB

(Lee et al., 2018)

AMPKα^GD736, Scer\GAL4^hs.PB has abnormal immune response | adult stage phenotype, suppressible by bmm^UAS.cUa, Scer\GAL4^hs.PB

(Lee et al., 2018)

AMPKα^GD736, Scer\GAL4^hs.PB has increased mortality | adult stage | conditional phenotype, suppressible by G6pd^UAS.cLa, Scer\GAL4^hs.PB

(Lee et al., 2018)

AMPKα^GD736, Scer\GAL4^hs.PB has abnormal immune response | adult stage phenotype, suppressible by G6pd^UAS.cLa, Scer\GAL4^hs.PB

(Lee et al., 2018)

Enhancer of

Statement

Reference

AMPKα^GD736, Scer\GAL4^Ddc.PL is an enhancer of abnormal locomotor behavior | adult stage phenotype of Hsap\LRRK2^{G2019S.UAS.Tag:MYC}, Scer\GAL4^Ddc.PL

(Ng et al., 2012)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

AMPKα^GD736, Scer\GAL4^Ddc.PL is a non-enhancer of dopaminergic PPL1 neuron phenotype of Hsap\LRRK2^{G2019S.UAS.Tag:MYC}, Scer\GAL4^Ddc.PL

(Ng et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of SNF1A^GD736 enhances the climbing defects seen in flies expressing Hsap\LRRK2^{G2019S.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Ddc.PL. No further loss of the protocerebral posterior lateral 1 (PPL1) dopaminergic neurons is seen. The beneficial effects of EGCG treatment are completely abolished.

(Ng et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v1827

w¹¹¹⁸; P{GD736}v1827

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

AMPKα^GD736

SNF1A^GD736

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

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