FlyBase Allele Report: Dmel\Vps25[GD8432]

General Information

Symbol

Dmel\Vps25^GD8432

Species

D. melanogaster

Name

FlyBase ID

FBal0208991

Feature type

allele

Associated gene

Dmel\Vps25

Associated Insertion(s)

Carried in Construct

P{GD8432}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD8432}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD8432

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

larval brain | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

male genitalia, with Scer\GAL4^Abd-B-LDN

(Lee et al., 2011)

Detailed Description

Statement

Reference

~30% of males expressing Vps25^GD8432 under the control of Scer\GAL4^Abd-B-LDN show incomplete genitalia rotation.

(Lee et al., 2011)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in an increased number and size of GFP-positive aggregates within neuroblast lineage cells in the larval brain compared to controls (these aggregates may indicate cell death or a disturbance of internal cellular structures).

(Neumüller et al., 2011)

Adults expressing Vps25^GD8432 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development