FlyBase Allele Report: Dmel\WASp[GD1559]

General Information

Symbol

Dmel\WASp^GD1559

Species

D. melanogaster

Name

FlyBase ID

FBal0208996

Feature type

allele

Associated gene

Dmel\WASp

Associated Insertion(s)

Carried in Construct

P{GD1559}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1559}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1559

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

neuromuscular junction, with Scer\GAL4^C57

(Nahm et al., 2010)

NMJ bouton, with Scer\GAL4^C57

(Nahm et al., 2010)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Expression of WASp^GD1559 in muscles using Scer\GAL4^C57 significantly increases bouton number, satellite bouton number, and total neuromuscular junction length.

Expression of WASp^GD1559 in neurons using Scer\GAL4^elav-C155 has no significant effect on synaptic growth.

(Nahm et al., 2010)

Adults expressing WASp^GD1559 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 20-30% or 30-40% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 60-70% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1559

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^C57, WASp^GD1559 has abnormal neuroanatomy phenotype, suppressible by wit^A12/wit[+]

(Nahm et al., 2010)

NOT Enhancer of

Statement

Reference

WASp^GD1559, Scer\GAL4^ptc.PU is a non-enhancer of visible | adult stage phenotype of Dora^GD6527, Scer\GAL4^ptc.PU

(Molina-Pelayo et al., 2022)

WASp^GD1559/Scer\GAL4^C57 is a non-enhancer of abnormal neuroanatomy phenotype of wit^B11/wit^A12

(Nahm et al., 2010)

NOT Suppressor of

Statement

Reference

WASp^GD1559, Scer\GAL4^ptc.PU is a non-suppressor of visible | adult stage phenotype of Dora^GD6527, Scer\GAL4^ptc.PU

(Molina-Pelayo et al., 2022)

WASp^GD1559/Scer\GAL4^C57 is a non-suppressor of abnormal neuroanatomy phenotype of wit^B11/wit^A12

(Nahm et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^C57, WASp^GD1559 has neuromuscular junction phenotype, suppressible by wit^A12/wit[+]

(Nahm et al., 2010)

Scer\GAL4^C57, WASp^GD1559 has bouton phenotype, suppressible by wit^A12/wit[+]

(Nahm et al., 2010)

NOT Enhancer of

Statement

Reference

WASp^GD1559, Scer\GAL4^ptc.PU is a non-enhancer of wing hair phenotype of Dora^GD6527, Scer\GAL4^ptc.PU

(Molina-Pelayo et al., 2022)

WASp^GD1559/Scer\GAL4^C57 is a non-enhancer of synapse phenotype of wit^B11/wit^A12

(Nahm et al., 2010)

WASp^GD1559/Scer\GAL4^C57 is a non-enhancer of neuromuscular junction phenotype of wit^B11/wit^A12

(Nahm et al., 2010)

NOT Suppressor of

Statement

Reference

WASp^GD1559, Scer\GAL4^ptc.PU is a non-suppressor of wing hair phenotype of Dora^GD6527, Scer\GAL4^ptc.PU

(Molina-Pelayo et al., 2022)

WASp^GD1559/Scer\GAL4^C57 is a non-suppressor of synapse phenotype of wit^B11/wit^A12

(Nahm et al., 2010)

WASp^GD1559/Scer\GAL4^C57 is a non-suppressor of neuromuscular junction phenotype of wit^B11/wit^A12

(Nahm et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of WASp^GD1559 post-synaptically (under the control of Scer\GAL4^C57) in wit^A12/wit^B11 larvae does not affect overall bouton number or satellite bouton formation.

A wit^A12 heterozygous background can completely reverse the increase in total bouton number and satellite bouton formation in larvae expressing WASp^GD1559 post-synaptically (under the control of Scer\GAL4^C57).

(Nahm et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v13757