FlyBase Allele Report: Dmel\brat[GD7073]

General Information

Symbol

Dmel\brat^GD7073

Species

D. melanogaster

Name

FlyBase ID

FBal0209175

Feature type

allele

Associated gene

Dmel\brat

Associated Insertion(s)

Carried in Construct

P{GD7073}

Also Known As

UAS-brat^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD7073}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD7073

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of brat^GD7073 under the combined control of Scer\GAL4^insc-Mz1407 and Dicer-2 (for efficient RNAi) frequently leads to brain tumors.

(Landskron et al., 2018)

The expression of brat^GD7073 in type II neuroblast under the combined control of Scer\GAL4^wor.PA, Scer\GAL80^ase.PN leads to larval brain tumors comprised of type II neuroblast-like cells, leads to invasive tumors in the adult central nervous system, and leads to significant adult lethality, as compared to controls. Expression induced only during the third instar larval stage (under the control of tub-Gal80[ts]) leads to a severe increase in the number of type II neuroblast lineage cells only after 72h of induction, as compared to controls.

(Reichardt et al., 2018)

Expression of brat^GD7073 under the control of Scer\GAL4^nub.PU (using UAS-Dicer2 to enhance the RNAi efficiency) does not cause any apparent adult wing defects.

(Miles et al., 2014)

Larvae expressing brat^GD7073 under the control of Scer\GAL4^elav.PU show an increase in total bouton number and in number of satellite boutons at the neuromuscular junction (NMJ) compared to wild type, while expression under the control of Scer\GAL4^Mhc.PW has no significant effect on the number of NMJ boutons.

(Shi et al., 2013)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in an increase in the number of neuroblasts in the larval brain compared to controls.

(Neumüller et al., 2011)

Adults expressing brat^GD7073 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

7073

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^insc-Mz1407, brat^GD7073 has neoplasia phenotype, suppressible by lncRNA:cherub^DEL/lncRNA:cherub^DEL

(Landskron et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has partially lethal - majority die phenotype, suppressible by grh^KK111269/Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has partially lethal - majority die phenotype, suppressible by Myc^KK103869, Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has partially lethal - majority die phenotype, suppressible by Scer\GAL4^wor.PA/dpn^KK101812, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has partially lethal - majority die phenotype, suppressible by Scer\GAL4^wor.PA/zld^GD7803, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has neoplasia | adult stage phenotype, suppressible by Scer\GAL4^wor.PA/zld^RNAi.all.UAS, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has neoplasia | adult stage phenotype, suppressible by Scer\GAL4^wor.PA/dpn^KK101812, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has neoplasia | larval stage phenotype, suppressible by Scer\GAL4^wor.PA/zld^RNAi.all.UAS, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has neoplasia | adult stage phenotype, suppressible by Scer\GAL4^wor.PA/dpn^RNAi.UAS, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has neoplasia | larval stage phenotype, suppressible by Scer\GAL4^wor.PA/dpn^RNAi.UAS, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has neoplasia | adult stage phenotype, suppressible by Scer\GAL4^wor.PA/zld^RNAi.RB.UAS, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has neoplasia | larval stage phenotype, suppressible by Scer\GAL4^wor.PA/zld^RNAi.RB.UAS, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has neoplasia | adult stage phenotype, suppressible by Scer\GAL4^wor.PA/zld^{RNAi.RD.1.UAS}, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has neoplasia | larval stage phenotype, suppressible by Scer\GAL4^wor.PA/zld^{RNAi.RD.1.UAS}, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has neoplasia | adult stage phenotype, suppressible by Scer\GAL4^wor.PA/zld^{RNAi.RD.2.UAS}, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has neoplasia | larval stage phenotype, suppressible by Scer\GAL4^wor.PA/zld^{RNAi.RD.2.UAS}, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has neoplasia | adult stage phenotype, suppressible by zld^{RNAi.RD.3.UAS}/Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has neoplasia | larval stage phenotype, suppressible by zld^{RNAi.RD.3.UAS}/Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

NOT suppressed by

Statement

Reference

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has partially lethal - majority die phenotype, non-suppressible by pnt^KK100473/Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has neoplasia | larval stage phenotype, non-suppressible by dpn^UAS.cBa/Scer\GAL4^wor.PA/zld^RNAi.all.UAS, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Other

Statement

Reference

Rbf^HMS03004, Scer\GAL4^nub.PU, brat^GD7073 has visible | adult stage phenotype

(Miles et al., 2014)

E2f2^JF02828, Scer\GAL4^nub.PU, brat^GD7073 has visible | adult stage phenotype

(Miles et al., 2014)

Scer\GAL4^nub.PU, brat^GD7073, mip120^HMS00461 has visible | adult stage phenotype

(Miles et al., 2014)

Scer\GAL4^nub.PU, brat^GD7073, mip130^HMS00462 has visible | adult stage phenotype

(Miles et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^insc-Mz1407, brat^GD7073 has brain phenotype, suppressible by lncRNA:cherub^DEL/lncRNA:cherub^DEL

(Landskron et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has adult brain phenotype, suppressible by Scer\GAL4^wor.PA/zld^RNAi.RB.UAS, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has larval brain phenotype, suppressible by Scer\GAL4^wor.PA/zld^{RNAi.RD.1.UAS}, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has adult brain phenotype, suppressible by Scer\GAL4^wor.PA/zld^{RNAi.RD.1.UAS}, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has larval brain phenotype, suppressible by Scer\GAL4^wor.PA/zld^{RNAi.RD.2.UAS}, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has adult brain phenotype, suppressible by zld^{RNAi.RD.3.UAS}/Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has larval brain phenotype, suppressible by zld^{RNAi.RD.3.UAS}/Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has type II neuroblast | third instar larval stage phenotype, suppressible by Scer\GAL4^wor.PA/zld^RNAi.all.UAS, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has type II neuroblast | third instar larval stage phenotype, suppressible by Scer\GAL4^wor.PA/dpn^RNAi.UAS, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has type II neuroblast | third instar larval stage phenotype, suppressible by Scer\GAL4^wor.PA/zld^RNAi.RB.UAS, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has type II neuroblast | third instar larval stage phenotype, suppressible by Scer\GAL4^wor.PA/zld^{RNAi.RD.1.UAS}, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has type II neuroblast | third instar larval stage phenotype, suppressible by Scer\GAL4^wor.PA/zld^{RNAi.RD.2.UAS}, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has type II neuroblast | third instar larval stage phenotype, suppressible by zld^{RNAi.RD.3.UAS}/Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has adult brain phenotype, suppressible by Scer\GAL4^wor.PA/zld^RNAi.all.UAS, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has adult brain phenotype, suppressible by Scer\GAL4^wor.PA/dpn^KK101812, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has larval brain phenotype, suppressible by Scer\GAL4^wor.PA/zld^RNAi.all.UAS, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has larval brain phenotype, suppressible by Scer\GAL4^wor.PA/dpn^RNAi.UAS, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has adult brain phenotype, suppressible by Scer\GAL4^wor.PA/dpn^RNAi.UAS, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has larval brain phenotype, suppressible by Scer\GAL4^wor.PA/zld^RNAi.RB.UAS, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

NOT suppressed by

Statement

Reference

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has larval brain phenotype, non-suppressible by dpn^UAS.cBa/Scer\GAL4^wor.PA/zld^RNAi.all.UAS, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^GD7073 has type II neuroblast | third instar larval stage phenotype, non-suppressible by dpn^UAS.cBa/Scer\GAL4^wor.PA/zld^RNAi.all.UAS, Scer\GAL80^ase.PN

(Reichardt et al., 2018)

Other

Statement

Reference

Rbf^HMS03004, Scer\GAL4^nub.PU, brat^GD7073 has wing blade phenotype

(Miles et al., 2014)

E2f2^JF02828, Scer\GAL4^nub.PU, brat^GD7073 has wing blade phenotype

(Miles et al., 2014)

Scer\GAL4^nub.PU, brat^GD7073, mip120^HMS00461 has wing blade phenotype

(Miles et al., 2014)

Scer\GAL4^nub.PU, brat^GD7073, mip130^HMS00462 has wing blade phenotype

(Miles et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The adult lethality of individuals expressing brat^GD7073 under the combined control of Scer\GAL4^wor.PA, Scer\GAL80^ase.PN is strongly suppressed by the co-expression of either dpn^KK101812 or zld^GD7803, is moderately suppressed by the co-expression of either Myc^KK103869 or grh^KK111269, but is not suppressed by the co-expression of pnt^KK100473.

The larval brain and adult brain tumor phenotypes (and metastatic capacity of xenografts into wild-type hosts) resulting from the expression of brat^GD7073 under the combined control of Scer\GAL4^wor.PA, Scer\GAL80^ase.PN are strongly suppressed by the single co-expression of zld^{shRNA.all.Scer\UAS}, dpn^{shRNA.Scer\UAS} or zld^{shRNA.RB.Scer\UAS}, but not of zld^{shRNA.RD.1.Scer\UAS}, zld^{shRNA.RD.2.Scer\UAS} or zld^{shRNA.RD.3.Scer\UAS}. The adult brain tumor phenotype is also strongly suppressed by the co-expression of dpn^KK101812. Although the larval brain tumor phenotype is suppressed by the co-expression of zld^{shRNA.all.Scer\UAS} alone, its is not suppressed by the additional co-expression of zld^{shRNA.all.Scer\UAS} and dpn^Scer\UAS.cBa.

(Reichardt et al., 2018)

Co-expression of any of the following: Rbf^HMS03004, E2f2^JF02828, mip130^HMS00462 or mip120^HMS00461 with brat^GD7073 under the control of Scer\GAL4^nub.PU (using UAS-Dicer2 to enhance RNAi efficiency) results in adult wing defects including blistering and severe wing deformation (with Rbf^HMS03004).