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General Information
Symbol
Dmel\bratGD7073
Species
D. melanogaster
Name
FlyBase ID
FBal0209175
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-bratRNAi
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of bratGD7073 under the combined control of Scer\GAL4insc-Mz1407 and Dicer-2 (for efficient RNAi) frequently leads to brain tumors.

The expression of bratGD7073 in type II neuroblast under the combined control of Scer\GAL4wor.PA, Scer\GAL80ase.PN leads to larval brain tumors comprised of type II neuroblast-like cells, leads to invasive tumors in the adult central nervous system, and leads to significant adult lethality, as compared to controls. Expression induced only during the third instar larval stage (under the control of tub-Gal80[ts]) leads to a severe increase in the number of type II neuroblast lineage cells only after 72h of induction, as compared to controls.

Expression of bratGD7073 under the control of Scer\GAL4nub.PU (using UAS-Dicer2 to enhance the RNAi efficiency) does not cause any apparent adult wing defects.

Larvae expressing bratGD7073 under the control of Scer\GAL4elav.PU show an increase in total bouton number and in number of satellite boutons at the neuromuscular junction (NMJ) compared to wild type, while expression under the control of Scer\GAL4Mhc.PW has no significant effect on the number of NMJ boutons.

Expression under the control of Scer\GAL41407 results in an increase in the number of neuroblasts in the larval brain compared to controls.

Adults expressing bratGD7073 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
NOT suppressed by
Other
Phenotype Manifest In
Suppressed by
Statement
Reference
NOT suppressed by
Other
Additional Comments
Genetic Interactions
Statement
Reference

The adult lethality of individuals expressing bratGD7073 under the combined control of Scer\GAL4wor.PA, Scer\GAL80ase.PN is strongly suppressed by the co-expression of either dpnKK101812 or zldGD7803, is moderately suppressed by the co-expression of either MycKK103869 or grhKK111269, but is not suppressed by the co-expression of pntKK100473.

The larval brain and adult brain tumor phenotypes (and metastatic capacity of xenografts into wild-type hosts) resulting from the expression of bratGD7073 under the combined control of Scer\GAL4wor.PA, Scer\GAL80ase.PN are strongly suppressed by the single co-expression of zldshRNA.all.Scer\UAS, dpnshRNA.Scer\UAS or zldshRNA.RB.Scer\UAS, but not of zldshRNA.RD.1.Scer\UAS, zldshRNA.RD.2.Scer\UAS or zldshRNA.RD.3.Scer\UAS. The adult brain tumor phenotype is also strongly suppressed by the co-expression of dpnKK101812. Although the larval brain tumor phenotype is suppressed by the co-expression of zldshRNA.all.Scer\UAS alone, its is not suppressed by the additional co-expression of zldshRNA.all.Scer\UAS and dpnScer\UAS.cBa.

Co-expression of any of the following: RbfHMS03004, E2f2JF02828, mip130HMS00462 or mip120HMS00461 with bratGD7073 under the control of Scer\GAL4nub.PU (using UAS-Dicer2 to enhance RNAi efficiency) results in adult wing defects including blistering and severe wing deformation (with RbfHMS03004).

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 2 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Neuroblasts Screen Database (Knoblich Lab) - A database for RNAi phenotypes in larval neural stem cells
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
bratGD7073
bratv31333
Name Synonyms
Secondary FlyBase IDs
    References (13)