FlyBase Allele Report: Dmel\egr[GD12658]

General Information

Symbol

Dmel\egr^GD12658

Species

D. melanogaster

Name

FlyBase ID

FBal0209506

Feature type

allele

Associated gene

Dmel\egr

Associated Insertion(s)

Carried in Construct

P{GD12658}

Also Known As

UAS-Egr RNAi, UAS-eiger-RNAi, UAS-egrRNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12658}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12658

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal starvation stress response | adult stage, with Scer\GAL4^ppl.PP

(Pathak and Varghese, 2021)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adults expressing egr^GD12658 under the control of Scer\GAL4^ppl.PP are resistant to starvation.

(Pathak and Varghese, 2021)

The expression of egr^GD12658 under the control of either Scer\GAL4^hh-Gal4 or Scer\GAL4^Hml.Δ does not lead to any obvious phenotype in the wing disc, as compared to controls.

(de Vreede et al., 2018)

Adults expressing egr^GD12658 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Flies co-expressing egr^Scer\UAS.cMa and egr^GD12658 under the control of Scer\GAL4^GMR.PF display normal eyes.

(Portela et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12658

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^ppl.PP, egr^GD12658 has abnormal starvation stress response | adult stage phenotype, suppressible by Edem1^GD3196, Scer\GAL4^ppl.PP

(Pathak and Varghese, 2021)

Suppressor of

Statement

Reference

Scer\GAL4^ptc-559.1/egr^GD12658 is a suppressor | partially of increased size | third instar larval stage phenotype of Scer\GAL4^ptc-559.1, Toll-7^{C993Y.UAS.Tag:FLAG}

(Ding et al., 2022)

Scer\GAL4^ptc-559.1/egr^GD12658 is a suppressor of abnormal cell migration | third instar larval stage phenotype of Scer\GAL4^ptc-559.1, Toll-7^{C993Y.UAS.Tag:FLAG}

(Ding et al., 2022)

egr^GD12658, Scer\GAL4^ppl.PP is a suppressor of abnormal starvation stress response | adult stage phenotype of Edem1^GD3196, Scer\GAL4^ppl.PP

(Pathak and Varghese, 2021)

egr^GD12658/Scer\GAL4^αTub84B.PL is a suppressor of neoplasia | larval stage phenotype of Alg3^tid2/Alg3^tid1

(de Vreede et al., 2018)

egr^GD12658/Scer\GAL4^r4 is a suppressor of neoplasia | larval stage phenotype of Alg3^tid2/Alg3^tid1

(de Vreede et al., 2018)

egr^GD12658, Scer\GAL4^elav-C155 is a suppressor of increased cell death | adult stage phenotype of Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155

(Wu et al., 2017)

egr^GD12658, Scer\GAL4^elav-C155 is a suppressor of abnormal locomotor behavior | adult stage phenotype of Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155

(Wu et al., 2017)

egr^GD12658, Scer\GAL4^elav-C155 is a suppressor of short lived phenotype of Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155

(Wu et al., 2017)

NOT Suppressor of

Statement

Reference

Scer\GAL4^hh-Gal4/egr^GD12658 is a non-suppressor of neoplasia | larval stage phenotype of Alg3^tid2/Alg3^tid1

(de Vreede et al., 2018)

Scer\GAL4^Hml.Δ/egr^GD12658 is a non-suppressor of neoplasia | larval stage phenotype of Alg3^tid2/Alg3^tid1

(de Vreede et al., 2018)

egr^GD12658, Scer\GAL4^Antp-10 is a non-suppressor of increased cell number | third instar larval stage phenotype of ND-75^GD6309, Scer\GAL4^Antp-10

(Sinenko et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^ptc-559.1/egr^GD12658 is a suppressor | partially of anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype of Scer\GAL4^ptc-559.1, Toll-7^{C993Y.UAS.Tag:FLAG}

(Ding et al., 2022)

egr^GD12658/Scer\GAL4^αTub84B.PL is a suppressor of wing disc phenotype of Alg3^tid2/Alg3^tid1

(de Vreede et al., 2018)

egr^GD12658/Scer\GAL4^r4 is a suppressor of wing disc phenotype of Alg3^tid2/Alg3^tid1

(de Vreede et al., 2018)

egr^GD12658, Scer\GAL4^elav-C155 is a suppressor of adult brain phenotype of Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155

(Wu et al., 2017)

NOT Suppressor of

Statement

Reference

Scer\GAL4^hh-Gal4/egr^GD12658 is a non-suppressor of wing disc phenotype of Alg3^tid2/Alg3^tid1

(de Vreede et al., 2018)

Scer\GAL4^Hml.Δ/egr^GD12658 is a non-suppressor of wing disc phenotype of Alg3^tid2/Alg3^tid1

(de Vreede et al., 2018)

egr^GD12658, Scer\GAL4^Antp-10 is a non-suppressor of lamellocyte | third instar larval stage phenotype of ND-75^GD6309, Scer\GAL4^Antp-10

(Sinenko et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of egr^GD12658 does not suppress the increase in circulating lamellocytes seen when ND75^GD6309 is expressed under the control of Scer\GAL4^Antp-10.

(Sinenko et al., 2011)

Expression of egr^GD12658 under the control of Scer\GAL4^tub.PU does not affect the reduction in l(2)gl⁴ mutant wing disc clone size compared with controls.

(Froldi et al., 2010)

Xenogenetic Interactions

Statement

Reference

Co-expression of egr^GD12658 partially suppresses the increased cell death in the brain, the climbing defects, and the shortened lifespan seen in Ecc15-infected flies expressing Hsap\APP^{Aβ42.Scer\UAS.cBa} under the control of Scer\GAL4^elav-C155.

(Wu et al., 2017)

Complementation and Rescue Data

Rescues

egr^GD12658/Scer\GAL4^GMR.PFa rescues egr^UAS.cMa

(Ruan et al., 2016)

Comments

The dot-like malformed adult eye phenotype seen in flies expressing egr^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PFa can be fully restored by co-expression of egr^GD12658 RNAi.

(Ruan et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v45252

w¹¹¹⁸; P{GD12658}v45252

v45253

w¹¹¹⁸; P{GD12658}v45253

Notes on Origin

Discoverer