FlyBase Allele Report: Dmel\for[GD6843]

General Information

Symbol

Dmel\for^GD6843

Species

D. melanogaster

Name

FlyBase ID

FBal0209583

Feature type

allele

Associated gene

Dmel\for

Associated Insertion(s)

Carried in Construct

P{GD6843}

Also Known As

UAS-dg2-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD6843}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD6843

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response | adult stage, with Scer\GAL4^c564

(Kanoh et al., 2021)

abnormal immune response | adult stage, with Scer\GAL4^Cg.PA

(Kanoh et al., 2021)

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^elav.PU

(Peng et al., 2016)

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

decreased sleep | adult stage, with Scer\GAL4^{GS.GMR54C07.BBB}

(Avila et al., 2025)

increased mortality | adult stage | conditional, with Scer\GAL4^c564

(Kanoh et al., 2021)

increased mortality | adult stage | conditional, with Scer\GAL4^Cg.PA

(Kanoh et al., 2021)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^GMR.PU

(Peng et al., 2016)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

axon | embryonic stage, with Scer\GAL4^elav.PU

(Peng et al., 2016)

eye, with Scer\GAL4^GMR.PU

(Peng et al., 2016)

larval intersegmental nerve branch ISNb of A1-7 | embryonic stage, with Scer\GAL4^elav.PU

(Peng et al., 2016)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

The expression of for^GD6843 under the control of Scer\GAL4^Cg.PA leads to increased susceptibility (i.e. mortality) of adults to infection with Staphylococcus saprophyticus bacteria.

The expression of for^GD6843 under the control of Scer\GAL4^c564 (together with Dicer-2 for a more efficient RNAi) leads increased susceptibility (i.e. mortality) of larvae to infection with Staphylococcus saprophyticus bacteria.

(Kanoh et al., 2021)

Expression of for^GD6843 driven by Scer\GAL4^elav.PU results in targeting defects (such as mistargeting) of motor axons of intersegmental nerve b (ISNb) in embryos. Expression of for^GD6843 driven by Scer\GAL4^GMR.PU results a rough eye phenotype.

(Peng et al., 2016)

Adults expressing for^GD6843 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) can show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies, depending on the particular P{GD6843} insertion line used.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or adult/pharate lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 50-60% or 90-100% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development