FlyBase Allele Report: Dmel\grp[GD4189]

General Information

Symbol

Dmel\grp^GD4189

Species

D. melanogaster

Name

FlyBase ID

FBal0209661

Feature type

allele

Associated gene

Dmel\grp

Associated Insertion(s)

Carried in Construct

P{GD4189}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD4189}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD4189

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA with BacA\p35^UAS.cHa

(Dekanty et al., 2015)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adults expressing grp^GD4189 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4189

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

grp^GD4189, Scer\GAL4^en.PU is an enhancer of hyperplasia | larval stage | conditional phenotype of BacA\p35^UAS.cHa, Scer\GAL4^en.PU

(Dekanty et al., 2015)

Suppressor of

Statement

Reference

grp^GD4189, Scer\GAL4^nub.PU is a suppressor | partially of visible | adult stage phenotype of His1^NIG.31617R, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

grp^GD4189, Scer\GAL4^nub.PU is a suppressor | partially of abnormal size | adult stage phenotype of His1^NIG.31617R, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

Phenotype Manifest In

Enhancer of

Statement

Reference

grp^GD4189, Scer\GAL4^en.PU is an enhancer of wing disc posterior compartment | conditional phenotype of BacA\p35^UAS.cHa, Scer\GAL4^en.PU

(Dekanty et al., 2015)

Suppressor of

Statement

Reference

grp^GD4189, Scer\GAL4^nub.PU is a suppressor | partially of wing phenotype of His1^NIG.31617R, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

grp^GD4189, Scer\GAL4^GMR.PF is a suppressor | partially of eye | temperature conditional phenotype of PEK^UAS.cMa, Scer\GAL4^GMR.PF

(Malzer et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The small and deformed wings in adult flies expressing His1:CG31617^NIG.31617R under the control of Scer\GAL4^nub.PU are significantly suppressed by co-expression of grp^GD4189.

(Bayona-Feliu et al., 2017)

The small, depigmented-eye phenotype of flies expressing PEK^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PF is partially suppressed by co-expression of grp^GD4189.

(Malzer et al., 2010)

Xenogenetic Interactions

Statement

Reference

The co-expression of grp^GD4189 enhances the enlarged wing disc posterior compartment observed upon exposure to ionizing radiation of individuals expressing BacA\p35^UAS.cHa under the control of Scer\GAL4^en.PU.

The Scer\GAL4^en.PU-driven co-expression of BacA\p35^UAS.cHa and grp^GD4189 leads to a longer sustained DNA damage response to ionizing ration (assessed by phospho-H2Av immunostaining) in the wing disc posterior compartment as compared to the BacA\p35^UAS.cHa single expression or wild-type backgrounds.

(Dekanty et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v12680

w¹¹¹⁸; P{GD4189}v12680/TM3

Notes on Origin

Discoverer