FlyBase Allele Report: Dmel\mts[GD12133]

General Information

Symbol

Dmel\mts^GD12133

Species

D. melanogaster

Name

FlyBase ID

FBal0210136

Feature type

allele

Associated gene

Dmel\mts

Associated Insertion(s)

Carried in Construct

P{GD12133}

Also Known As

UAS-mts RNAi, UAS-MtsRNAi, mts-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12133}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12133

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates amyotrophic lateral sclerosis type 6

modeled by Hsap\FUS^UAS.cBa

(Tziortzouda et al., 2024)

modeled by Hsap\FUS^{R521G.UAS.cBa}

(Tziortzouda et al., 2024)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

larval brain, with Scer\GAL4^ase.neuro

(Wang et al., 2009)

larval brain, with Scer\GAL4^insc-Mz1407

(Wang et al., 2009)

neuroblast | increased number, with Scer\GAL4^ase.neuro

(Wang et al., 2009)

neuroblast | increased number, with Scer\GAL4^insc-Mz1407

(Wang et al., 2009)

Detailed Description

Statement

Reference

Expression of mts^GD12133 under the control of Scer\GAL4^Act.PU in clones of larval fat body cells results in inhibition of autophagy.

(Bánréti et al., 2012)

Knockdown of mts in the developing brain through expression of mts^GD12133 under the control of Scer\GAL4^Dll-md23 has no effect on type II neuroblasts.

(Ouyang et al., 2011)

Adults expressing mts^GD12133 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) can show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies, depending on the particular P{GD12133} insertion line used.

Adults expressing mts^GD12133 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show no significant change in the kinetics of high temperature (46[o]C) induced paralysis compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or late larval lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal larval body wall muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in wild-type sarcomeres in the larval body wall muscles.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in lethality, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

Expression of mts^GD12133 under the control of Scer\GAL4¹⁴⁰⁷ or Scer\GAL4^ase.neuro results in neuroblast proliferation in the larval brain.

Expression of mts^GD12133 under the control of Scer\GAL4¹⁴⁰⁷ at 13[o]C or 18[o]C results in more BrdU incorporation in the larval brain compared to controls. However, expression of mts^GD12133 under the control of Scer\GAL4¹⁴⁰⁷ at 25[o]C or 30[o]C results in less BrdU incorporation in the larval brain compared to controls.

(Wang et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12133

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^Dll-md23, mts^GD12133, polo^UAS.N.GFP has abnormal neuroanatomy phenotype, enhanceable by Dronc^RNAi.UAS.Ka, Scer\GAL4^Dll-md23

(Ouyang et al., 2011)

Other

Statement

Reference

Scer\GAL4^Dll-md23, mts^GD12133, polo^UAS.N.GFP has abnormal neuroanatomy phenotype

(Ouyang et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^Dll-md23, mts^GD12133, polo^UAS.N.GFP has neuroblast | increased number phenotype, enhanceable by Dronc^RNAi.UAS.Ka, Scer\GAL4^Dll-md23

(Ouyang et al., 2011)

Other

Statement

Reference

Scer\GAL4^Dll-md23, mts^GD12133, polo^UAS.N.GFP has neuroblast | increased number phenotype

(Ouyang et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of mts^GD12133 and polo^{Scer\UAS.N.T:Avic\GFP} in the brain, under the control of Scer\GAL4^Dll-md23, results in a 25% increase of ectopic neuroblasts. Co-expression of Nc^{dsRNA.Scer\UAS} increases this ectopic neuroblast formation by approximately 25%.

(Ouyang et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

VDRC

v35171