FlyBase Allele Report: Dmel\na[GD1172]

General Information

Symbol

Dmel\na^GD1172

Species

D. melanogaster

Name

FlyBase ID

FBal0210170

Feature type

allele

Associated gene

Dmel\na

Associated Insertion(s)

Carried in Construct

P{GD1172}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1172}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1172

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with Scer\GAL4^elav.PLu

(Dietzl et al., 2007)

abnormal flight, with Scer\GAL4^Mef2.PR

(Dietzl et al., 2007)

abnormal locomotor behavior, with Scer\GAL4^elav.PLu

(Dietzl et al., 2007)

abnormal locomotor behavior, with Scer\GAL4^Mef2.PR

(Dietzl et al., 2007)

abnormal neuroanatomy | larval stage, with Dcr-2^UAS.cDa, Scer\GAL4^1003.3

(Tsubouchi et al., 2012)

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

abnormal touch response, with Scer\GAL4^109(2)80

(Tsubouchi et al., 2012)

abnormal touch response, with Scer\GAL4^1003.3

(Tsubouchi et al., 2012)

abnormal touch response | larval stage, with Dcr-2^UAS.cDa, Scer\GAL4^1003.3

(Tsubouchi et al., 2012)

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW

(Tsubouchi et al., 2012)

lethal, with Scer\GAL4^Act5C.PU

(Dietzl et al., 2007)

lethal, with Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH

(Dietzl et al., 2007)

lethal, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

lethal, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010, Dietzl et al., 2007)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009, Dietzl et al., 2007)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Larvae expressing na^GD1172 under the control of Scer\GAL4^1003.3 (in the presence of Dcr-2^Scer\UAS.cDa to enhance RNAi) show a reduced response to gentle touch compared to controls. Class III ddaA neurons in these larvae show a significant decrease in the number of dendritic sensory filopodia compared to wild type. The average length of the filopodia is significantly decreased, while the total length of primary dendritic branches is not altered.

(Tsubouchi et al., 2012)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

(Neumüller et al., 2011)

Adults expressing na^GD1172 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in adult/pharate lethality or late pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 80-90% or 90-100% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

Expression of na^GD1172 under the control of Scer\GAL4^Bx-MS1096 results in crinkled wings.

Expression of na^GD1172 under the control of Scer\GAL4^pnr-MD237 produces a defective thorax closure phenotype.

(Dietzl et al., 2007)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development