FlyBase Allele Report: Dmel\pbl[GD12384]

General Information

Symbol

Dmel\pbl^GD12384

Species

D. melanogaster

Name

FlyBase ID

FBal0210302

Feature type

allele

Associated gene

Dmel\pbl

Associated Insertion(s)

Carried in Construct

P{GD12384}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12384}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12384

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

larval intersegmental nerve branch ISNb of A1-7, with Scer\GAL4^elav.PLu

(Jeong et al., 2012)

larval intersegmental nerve branch ISNb of A1-7, with Scer\GAL4^sca-537.4

(Jeong et al., 2012)

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Expression of pbl^GD12384 under the control of Scer\GAL4^sca-537.4 results in ISNb axons with increased fasciculation and defects in target recognition in 49.2% of hemisegments.

Expression of pbl^GD12384 under the control of Scer\GAL4^how-24B does not result in significant ISNb pathfinding defects.

Expression of pbl^GD12384 under the control of Scer\GAL4^elav.PLu results in ISNb guidance defects in 38% of hemisegments.

(Jeong et al., 2012)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in an extreme increase in neuroblast size in the larval brain, without loss of neuroblasts.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in extreme defects in the shape of neuroblasts in the larval brain.

(Neumüller et al., 2011)

Adults expressing pbl^GD12384 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) can show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies, depending on the particular P{GD12384} insertion line used.

Adults expressing pbl^GD12384 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show no significant change in the kinetics of high temperature (46[o]C) induced paralysis compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 may result in strong thorax closure defects, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development