FlyBase Allele Report: Dmel\rpk[GD3621]

General Information

Symbol

Dmel\rpk^GD3621

Species

D. melanogaster

Name

FlyBase ID

FBal0210489

Feature type

allele

Associated gene

Dmel\rpk

Associated Insertion(s)

Carried in Construct

P{GD3621}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD3621}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD3621

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

abnormal neuroanatomy | larval stage, with Dcr-2^UAS.cDa, Scer\GAL4^1003.3

(Tsubouchi et al., 2012)

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

abnormal size | adult stage, with Scer\GAL4^nub.PU

(Hevia et al., 2017)

abnormal touch response, with Scer\GAL4^109(2)80

(Tsubouchi et al., 2012)

abnormal touch response, with Scer\GAL4^1003.3

(Tsubouchi et al., 2012)

abnormal touch response | larval stage, with Dcr-2^UAS.cDa, Scer\GAL4^1003.3

(Tsubouchi et al., 2012)

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW

(Tsubouchi et al., 2012)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

larval dorsal multidendritic neuron ddaA | larval stage, with Dcr-2^UAS.cDa, Scer\GAL4^1003.3

(Tsubouchi et al., 2012)

sensory dendrite | larval stage, with Dcr-2^UAS.cDa, Scer\GAL4^1003.3

(Tsubouchi et al., 2012)

wing, with Scer\GAL4^nub.PU

(Hevia et al., 2017)

Detailed Description

Statement

Reference

The expression of rpk^GD3621 under the control of Scer\GAL4^nub.PU (together with Dicer-2, for efficient RNAi) leads to small wings compared to controls.

(Hevia et al., 2017)

Larvae expressing rpk^GD3621 under the control of Scer\GAL4^1003.3 (in the presence of Dcr-2^Scer\UAS.cDa to enhance RNAi) show a reduced response to gentle touch compared to controls. Class III ddaA neurons in these larvae show a significant decrease in the number of dendritic sensory filopodia compared to wild type. The average length of the filopodia is significantly decreased, while the total length of primary dendritic branches is not altered.

(Tsubouchi et al., 2012)

Adults expressing rpk^GD3621 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in flightless flies or a weak flyer phenotype.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in morphologically wild-type myofibrils in the adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in wild-type sarcomeres in the adult indirect flight muscles.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in lethality, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

3621

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

rpk^GD3621/Scer\GAL4^nub.PU is a suppressor of abnormal size | adult stage phenotype of Scer\GAL4^nub.PU, Smox^SDVD.UAS

(Hevia et al., 2017)

Phenotype Manifest In

Suppressor of

Statement

Reference

rpk^GD3621/Scer\GAL4^nub.PU is a suppressor of wing phenotype of Scer\GAL4^nub.PU, Smox^SDVD.UAS

(Hevia et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of rpk^GD3621 (together with Dicer-2, for efficient RNAi) suppresses the oversized wing defects induced by the expression of Smox^{SDVD.Scer\UAS} under the control of Scer\GAL4^nub.PU.

(Hevia et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v8549

w¹¹¹⁸; P{GD3621}v8549

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

3621

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

rpk^GD3621

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

Open Close