FlyBase Allele Report: Dmel\Sar1[GD10593]

General Information

Symbol

Dmel\Sar1^GD10593

Species

D. melanogaster

Name

FlyBase ID

FBal0210510

Feature type

allele

Associated gene

Dmel\Sar1

Associated Insertion(s)

Carried in Construct

P{GD10593}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD10593}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD10593

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cytokinesis | spermatogenesis, with Scer\GAL4^VP16.bam

(Matsuura et al., 2024)

abnormal meiotic cell cycle | spermatogenesis, with Scer\GAL4^VP16.bam

(Matsuura et al., 2024)

abnormal neuroanatomy | prepupal stage P1 | somatic clone, with Scer\GAL4^ppk.PU

(Wang et al., 2018)

abnormal neuroanatomy | pupal stage | somatic clone, with Scer\GAL4^ppk.PU

(Wang et al., 2018)

decreased body size | larval stage, with Scer\GAL4^Mef2.PR

(Langer et al., 2010)

lethal, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

lethal - all die before end of larval stage, with Scer\GAL4^Mef2.PR

(Langer et al., 2010)

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

partially lethal, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

some die during larval stage, with Scer\GAL4^Mef2.PR

(Langer et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

Phenotype Manifest In

contractile ring | spermatogenesis, with Scer\GAL4^VP16.bam

(Matsuura et al., 2024)

dendritic tree | prepupal stage P1 | somatic clone, with Scer\GAL4^ppk.PU

(Wang et al., 2018)

dendritic tree | pupal stage | somatic clone, with Scer\GAL4^ppk.PU

(Wang et al., 2018)

filamentous actin | spermatogenesis, with Scer\GAL4^VP16.bam

(Matsuura et al., 2024)

larval dorsal multidendritic neuron ddaC | prepupal stage P1 | somatic clone, with Scer\GAL4^ppk.PU

(Wang et al., 2018)

larval dorsal multidendritic neuron ddaC | pupal stage | somatic clone, with Scer\GAL4^ppk.PU

(Wang et al., 2018)

male germ cell nucleus | increased number, with Scer\GAL4^VP16.bam

(Matsuura et al., 2024)

sarcomere, with Scer\GAL4^Mef2.PR

(Langer et al., 2010, Schnorrer et al., 2010)

spermatid, with Scer\GAL4^VP16.bam

(Matsuura et al., 2024)

Z disc, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

Detailed Description

Statement

Reference

The expression of Sar1^GD10593 under the control of Scer\GAL4^ppk.PU leads to ddaC neurons showing significantly simplified dendrite arbors with fewer dendritic termini in white pre-pupae and showing strong pruning defects in pupae (16h APF).

(Wang et al., 2018)

Knockdown of Sar1 in the fat body, through expression of Sar1^GD10593 under the control of Scer\GAL4^Cg.PA causes retention of Avic\GFP^vkg-G454 in fat body cells. Avic\GFP^vkg-G454 accumulates in growing intracellular aggregates. These eventually coalesce and occupy most of the cytoplasm between the lipid droplets, affecting cell viability. These larvae lack Avic\GFP^vkg-G454 in the basement membranes of other organs, such as the wing disc.

(Pastor-Pareja and Xu, 2011)

Larvae expressing sar1^GD10593 under the control of Scer\GAL4^Mef2.PR show sarcomere defects. The larvae are also shorter in length than control larvae.

(Langer et al., 2010)

Adults expressing Sar1^GD10593 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in late larval lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal larval body wall muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in sarcomeres with reduced Z-lines in the larval body wall muscles.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality before the pupal stage.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10593

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The sarcomere defects and growth defects seen in larvae expressing sar1^GD10593 under the control of Scer\GAL4^Mef2.PR are completely rescued if they are carrying the P{FlyFos-045459} construct (which contains D. pseudoobscura pseudoobscura fosmid sequence that includes the region orthologous to sar1).

(Langer et al., 2010)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v34191

w¹¹¹⁸; P{GD10593}v34191

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10593

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Sar1^GD10593

sar1^GD10593

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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