FlyBase Allele Report: Dmel\scaf[GD5263]

General Information

Symbol

Dmel\scaf^GD5263

Species

D. melanogaster

Name

FlyBase ID

FBal0210524

Feature type

allele

Associated gene

Dmel\scaf

Associated Insertion(s)

Carried in Construct

P{GD5263}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD5263}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD5263

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adults expressing scaf^GD5263 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 20-30% of the Scer\GAL4^pnr-MD237 expression domain.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

5263

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Other

Statement

Reference

Dcr-2^UAS.cDa, Scer\GAL4^69B, scaf^GD5263 has embryonic/first instar larval cuticle phenotype

(Rousset et al., 2010)

Dcr-2^UAS.cDa, Scer\GAL4^ptc-559.1, scaf^GD5263 has adult head phenotype

(Rousset et al., 2010)

Dcr-2^UAS.cDa, Scer\GAL4^ptc-559.1, scaf^GD5263 has wing vein phenotype

(Rousset et al., 2010)

Dcr-2^UAS.cDa, Scer\GAL4^ptc-559.1, scaf^GD5263 has adult thoracic sensillum phenotype

(Rousset et al., 2010)

Dcr-2^UAS.cDa, Scer\GAL4^ptc-559.1, scaf^GD5263 has male genitalia phenotype

(Rousset et al., 2010)

Dcr-2^UAS.cDa, Scer\GAL4^ptc-559.1, scaf^GD5263 has cercus phenotype

(Rousset et al., 2010)

Dcr-2^UAS.cDa, Scer\GAL4^Abd-B-LDN, scaf^GD5263 has male genitalia phenotype

(Rousset et al., 2010)

Dcr-2^UAS.cDa, Scer\GAL4^Abd-B-LDN, scaf^GD5263 has cercus phenotype

(Rousset et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Embryos co-expressing scaf^GD5263 and Dcr-2^Scer\UAS.cDa driven by Scer\GAL4^69B display dorsal cuticle defects.

Flies co-expressing scaf^GD5263 and Dcr-2^Scer\UAS.cDa driven by Scer\GAL4^ptc-559.1 display head scars under the proboscis, loss of wing veins and thorax mechanosensory bristles, as well as male terminal malformations. The male terminalia phenotypes include genitalia rotation defects and partial dissociation of the genital plate from the abdomen. The same male terminalia phenotypes are seen when scaf^GD5263 and Dcr-2^Scer\UAS.cDa are co-expressed driven by Scer\GAL4^Abd-B-LDN.

(Rousset et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v13249

w¹¹¹⁸; P{GD5263}v13249

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

5263

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

scaf^GD5263

scarface^GD5263

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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