FlyBase Allele Report: Dmel\Mlf[ΔC1]

General Information

Symbol

Dmel\Mlf^ΔC1

Species

D. melanogaster

Name

FlyBase ID

FBal0213086

Feature type

allele

Associated gene

Dmel\Mlf

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Martin-Lanneree et al., 2006)

Progenitor genotype

Mlf^EU2490

(Martin-Lanneree et al., 2006)

Cytology

Description

Imprecise excision of the P{EP} insertion in Mlf^EU2490, resulting in a 1.8kb deletion removing nucleotides +443 to +2258 of Mlf (coordinates relative to the transcription start site of isoforms A,B and D).

(Martin-Lanneree et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | recessive | third instar larval stage

(Miller et al., 2017)

increased cell size | dominant | third instar larval stage

(Miller et al., 2017)

lethal - all die during embryonic stage | rescuable maternal effect

(Martin-Lanneree et al., 2006)

some die during embryonic stage | rescuable maternal effect

(Martin-Lanneree et al., 2006)

visible

(Martin-Lanneree et al., 2006)

Phenotype Manifest In

adult head bristle

(Martin-Lanneree et al., 2006)

embryonic/larval crystal cell | embryonic stage 15 (with Mlf^Δ5-3)

(Bras et al., 2012)

embryonic/larval crystal cell | third instar larval stage

(Miller et al., 2017)

interocellar bristle

(Martin-Lanneree et al., 2006)

wing vein | ectopic

(Martin-Lanneree et al., 2006)

Detailed Description

Statement

Reference

Mlf^ΔC1 homozygous third instar larvae present a significant increase in both the number and size of circulating crystal cells (identified as Lozenge-positive cells, and which are still capable of melanization upon heat treatment), but other circulating cells have unaffected size, as compared to controls; Mlf^ΔC1 heterozygotes also exhibit a (dominant) increase in the size, but not number, of crystal cells, as compared to controls.

(Miller et al., 2017)

Crystal cell maintenance is impaired in Mlf^Δ5-3/Mlf^ΔC1 embryos.

(Bras et al., 2012)

64.0% of animals derived from a cross of homozygous females to homozygous males die as embryos, whereas 46.5% of animals derived from a cross of homozygous females to wild-type males die, indicating that that maternal effect lethality is partially rescued by a paternal Mlf⁺ copy. Embryos derived from a cross of heterozygous females to wild-type males have normal viability.

90.0% of animals derived from a cross of homozygous Mlf^Δ5-3 females to homozygous Mlf^ΔC1 males die as embryos.

Homozygous adults lack interocellar bristles and the head macrochaetae are frequently bent or shortened. Ectopic venation is seen in the wing near longitudinal vein 2.

(Martin-Lanneree et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Mlf^ΔC1 has increased cell size | third instar larval stage | dominant phenotype, enhanceable by DnaJ-1[+]/DnaJ-1^A

(Miller et al., 2017)

Suppressed by

Statement

Reference

Mlf^ΔC1 has increased cell size | dominant | third instar larval stage phenotype, suppressible by lz^UAS.cBa/Scer\GAL4^lz-gal4

(Miller et al., 2017)

Mlf^ΔC1 has increased cell number | dominant | third instar larval stage phenotype, suppressible by lz^UAS.cBa/Scer\GAL4^lz-gal4

(Miller et al., 2017)

Mlf^ΔC1 has increased cell number | third instar larval stage phenotype, suppressible by N[+]/N^55e11

(Miller et al., 2017)

Mlf^ΔC1 has increased cell size | third instar larval stage phenotype, suppressible by N[+]/N^55e11

(Miller et al., 2017)

Mlf^ΔC1 has visible phenotype, suppressible by Scer\GAL4^GMR.PF/Hsap\MLF1^UAS.cMa

(Martin-Lanneree et al., 2006)

Enhancer of

Statement

Reference

Mlf[+]/Mlf^ΔC1 is an enhancer of increased cell size | third instar larval stage | dominant phenotype of DnaJ-1^A

(Miller et al., 2017)

NOT Suppressor of

Statement

Reference

Mlf[+]/Mlf^ΔC1 is a non-suppressor of short lived | RU486 conditional phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^{elav.Switch.PO}

(Martin-Lanneree et al., 2006)

Other

Statement

Reference

DnaJ-1^A, Mlf[+]/Mlf^ΔC1 has increased cell number | third instar larval stage phenotype

(Miller et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Mlf^ΔC1 has embryonic/larval crystal cell | third instar larval stage phenotype, enhanceable by DnaJ-1[+]/DnaJ-1^A

(Miller et al., 2017)

Suppressed by

Statement

Reference

Mlf^ΔC1 has embryonic/larval crystal cell | third instar larval stage phenotype, suppressible by lz^UAS.cBa/Scer\GAL4^lz-gal4

(Miller et al., 2017)

Mlf^ΔC1 has embryonic/larval crystal cell | third instar larval stage phenotype, suppressible by N[+]/N^55e11

(Miller et al., 2017)

Mlf^ΔC1 has macrochaeta phenotype, suppressible by Scer\GAL4^GMR.PF/Hsap\MLF1^UAS.cMa

(Martin-Lanneree et al., 2006)

Mlf^ΔC1 has wing phenotype, suppressible by Scer\GAL4^GMR.PF/Hsap\MLF1^UAS.cMa

(Martin-Lanneree et al., 2006)

Enhancer of

Statement

Reference

Mlf[+]/Mlf^ΔC1 is an enhancer of embryonic/larval crystal cell | third instar larval stage phenotype of DnaJ-1^A

(Miller et al., 2017)

Other

Statement

Reference

DnaJ-1^A, Mlf[+]/Mlf^ΔC1 has embryonic/larval crystal cell | third instar larval stage phenotype

(Miller et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

DnaJ-1^A, Mlf^ΔC1 double heterozygous third instar larvae present a significant increase in the number of circulating crystal cells (identified as Lozenge-positive cells), as compared to heterozygous or wild-type controls. DnaJ-1^A, Mlf^ΔC1 double heterozygous third instar larvae present a significant increase in the size of circulating crystal cells (identified as Lozenge-positive cells), as compared to each heterozygous or wild-type controls.

The increased number and size of circulating crystal cells observed in Mlf^ΔC1 homozygous third instar larvae is suppressed by the expression of lz^Scer\UAS.cBa under the control of Scer\GAL4^lz-gal4 or by N^55e11 heterozygosity.

(Miller et al., 2017)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\MLF1^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PF almost completely rescues the macrochaete and wing phenotypes seen in Mlf^ΔC1 homozygotes. The reduction in life span that is seen in flies expressing Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^{elav.Switch.PO} in the presence of RU486 is not modified by the addition of Mlf^ΔC1/+.

(Martin-Lanneree et al., 2006)

Complementation and Rescue Data

Rescued by

Mlf^Δ5-3/Mlf^ΔC1 is rescued by Mlf^UAS.cFa

(Bras et al., 2012)

Comments

Expression of Mlf^Scer\UAS.cFa under the control of Scer\GAL4^lz-gal4 rescues the crystal cell phenotype of Mlf^Δ5-3/Mlf^ΔC1 mutants.

(Bras et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Mlf^ΔC1

dmlf^ΔC1

(Martin-Lanneree et al., 2006)

mlf^ΔC1

(Miller et al., 2017, Bras et al., 2012)

mlf^δC1

(Miller et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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