FlyBase Allele Report: Dmel\DnaJ-1[A]

General Information

Symbol

Dmel\DnaJ-1^A

Species

D. melanogaster

Name

FlyBase ID

FBal0336286

Feature type

allele

Associated gene

Dmel\DnaJ-1

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Miller et al., 2017)

Mutagen

CRISPR/Cas9

(Miller et al., 2017)

Progenitor genotype

Cytology

Description

Complete deletion of the DnaJ-1 coding sequence.

(Miller et al., 2017)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval crystal cell | third instar larval stage

(Miller et al., 2017)

embryonic/larval crystal cell | third instar larval stage (with Df(3L)BSC884)

(Miller et al., 2017)

embryonic/larval crystal cell | third instar larval stage (with DnaJ-1^C)

(Miller et al., 2017)

Detailed Description

Statement

Reference

DnaJ-1^A/DnaJ-1^C transheterozygotes are partially lethal, since only a small minority reaches adulthood, but do not show obvious morphological defects. DnaJ-1^A homozygous, DnaJ-1^A/DnaJ-1^C transheterozygous or DnaJ-1^A/Df(3L)BSC884 transheterozygous third instar larvae present a significant increase in both the number and size of circulating crystal cells (identified as Lozenge-positive or PPO1-positive cells, which are still capable of melanization upon heat treatment), while other circulating cells have unaffected size, as compared to controls; heterozygotes also exhibit an increase in the size, but not number, of crystal cells, as compared to controls.

(Miller et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

DnaJ-1^A has increased cell size | third instar larval stage | dominant phenotype, enhanceable by Mlf[+]/Mlf^ΔC1

(Miller et al., 2017)

Suppressed by

Statement

Reference

DnaJ-1^C/DnaJ-1^A has increased cell size | third instar larval stage phenotype, suppressible by lz^UAS.cBa/Scer\GAL4^lz-gal4

(Miller et al., 2017)

DnaJ-1^C/DnaJ-1^A has increased cell number | third instar larval stage phenotype, suppressible by lz^UAS.cBa/Scer\GAL4^lz-gal4

(Miller et al., 2017)

DnaJ-1^C/DnaJ-1^A has increased cell size | third instar larval stage phenotype, suppressible by N[+]/N^55e11

(Miller et al., 2017)

DnaJ-1^C/DnaJ-1^A has increased cell number | third instar larval stage phenotype, suppressible by N[+]/N^55e11

(Miller et al., 2017)

Enhancer of

Statement

Reference

DnaJ-1[+]/DnaJ-1^A is an enhancer of increased cell size | third instar larval stage | dominant phenotype of Mlf^ΔC1

(Miller et al., 2017)

Other

Statement

Reference

DnaJ-1^A, Mlf[+]/Mlf^ΔC1 has increased cell number | third instar larval stage phenotype

(Miller et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

DnaJ-1^A has embryonic/larval crystal cell | third instar larval stage phenotype, enhanceable by Mlf[+]/Mlf^ΔC1

(Miller et al., 2017)

Suppressed by

Statement

Reference

DnaJ-1^C/DnaJ-1^A has embryonic/larval crystal cell | third instar larval stage phenotype, suppressible by lz^UAS.cBa/Scer\GAL4^lz-gal4

(Miller et al., 2017)

DnaJ-1^C/DnaJ-1^A has embryonic/larval crystal cell | third instar larval stage phenotype, suppressible by N[+]/N^55e11

(Miller et al., 2017)

Enhancer of

Statement

Reference

DnaJ-1[+]/DnaJ-1^A is an enhancer of embryonic/larval crystal cell | third instar larval stage phenotype of Mlf^ΔC1

(Miller et al., 2017)

Other

Statement

Reference

DnaJ-1^A, Mlf[+]/Mlf^ΔC1 has embryonic/larval crystal cell | third instar larval stage phenotype

(Miller et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

DnaJ-1^A, Mlf^ΔC1 double heterozygous third instar larvae present a significant increase in the number of circulating crystal cells (identified as Lozenge-positive cells), as compared to heterozygous or wild-type controls. DnaJ-1^A, Mlf^ΔC1 double heterozygous third instar larvae present a significant increase in the size of circulating crystal cells (identified as Lozenge-positive cells), as compared to each heterozygous or wild-type controls.

The increased number and size of circulating crystal cells observed in DnaJ-1^A/DnaJ-1^C transheterozygous third instar larvae is suppressed by the expression of lz^Scer\UAS.cBa under the control of Scer\GAL4^lz-gal4 or by N^55e11 heterozygosity.

(Miller et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

DnaJ-1^C/DnaJ-1^A is rescued by DnaJ-1^UAS.cKa/Scer\GAL4^lz-gal4

(Miller et al., 2017)

Not rescued by

DnaJ-1^C/DnaJ-1^A is not rescued by DnaJ-1^{ΔJ.UAS.Tag:FLAG}/Scer\GAL4^lz-gal4

(Miller et al., 2017)

Comments

The increased number and size of circulating crystal cells present in DnaJ-1^A/DnaJ-1^C transheterozygous third instar larvae are rescued by the expression of DnaJ-1^Scer\UAS.cKa, but not of DnaJ-1^{ΔJ.Scer\UAS.T:Zzzz\FLAG}, under the control of Scer\GAL4^lz-gal4.

(Miller et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

DnaJ-1^A

dnaj-1^A

(Miller et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

Open Close