FlyBase Allele Report: Dmel\lz[UAS.cBa]

General Information

Symbol

Dmel\lz^UAS.cBa

Species

D. melanogaster

Name

FlyBase ID

FBal0336288

Feature type

allele

Associated gene

Dmel\lz

Associated Insertion(s)

Carried in Construct

P{UAS-lz.B}

Also Known As

UAS-lz

Key Links

Transgenic product class

wild_type

(Sinenko et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Sinenko et al., 2010)

Mutagen

in vitro construct

(Sinenko et al., 2010, Tracey et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-lz.B}

Cytology

Description

UASt regulatory sequences drive expression of lz.

(Sinenko et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by yki^{S168A.UAS.cUa}

(Jang et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage, with Scer\GAL4^nanos.PG

(Tracey et al., 2000)

some die during embryonic stage, with Scer\GAL4^nanos.PG

(Tracey et al., 2000)

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of lz^Scer\UAS.cBa under the control of Scer\GAL4^lz-gal4 does not significantly affect the number or size of third instar larval circulating crystal cells.

(Miller et al., 2017)

Expression of lz^Scer\UAS.cSa under the control of Scer\GAL4^Hml.Δ has no effect on third instar larval hemocyte numbers.

(Sinenko et al., 2010)

Ectopic expression of lz^Scer\UAS.cBa, under the control of Scer\GAL4^srp.D.cCa in all of the prohemocytes, in a Df(2L)200 mutant background (which removes gcm and gcm2) results in almost all of the hemocytes remaining around the proventriculus. These hemocytes morphologically resemble differentiated crystal cells.

(Bataille et al., 2005)

The eyes of flies expressing lz^Scer\UAS.cBa under the control of Scer\GAL4^lz-gal4 appear wild type. When lz^Scer\UAS.cBa is expressed under the control of Scer\GAL4^GMR.PF, eyes are mildly rough.

(Wildonger and Mann, 2005)

When lz^Scer\UAS.cBa is driven by Scer\GAL4^twi.PB supernumerary crystal cells are seen that persist during embryogenesis. When lz^Scer\UAS.cBa is driven by Scer\GAL4^twi.PB an increased number of crystal cells are seen in the lymph glands.

(Fossett et al., 2003)

In lz^Scer\UAS.cBa; Scer\GAL4^svr-PG33 embryos, plasmatocytes express various crystal cell markers, but retain plasmatocyte morphology and marker expression. Crystal cell marker expression is not induced in non-hemocytes in lz^Scer\UAS.cBa; Scer\GAL4^da.G32 embryos. Crystal cell marker expression is increased in lz^Scer\UAS.cBa; Scer\GAL4^twi.PG embryos, but the extent of its expression within the mesoderm is limited. Crystal cell differentiation is normal in lz^Scer\UAS.cBa; Scer\GAL4^lz-gal4 embryos.

(Waltzer et al., 2003)

Expression of lz^Scer\UAS.cBa under the control of Scer\GAL4^nos.PG results in embryos with a dorsal closure defect or "tail-up" phenotype.

(Tracey et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

lz^UAS.cBa/Scer\GAL4^lz-gal4 is a suppressor of increased cell size | dominant | third instar larval stage phenotype of Mlf^ΔC1

(Miller et al., 2017)

lz^UAS.cBa/Scer\GAL4^lz-gal4 is a suppressor of increased cell number | dominant | third instar larval stage phenotype of Mlf^ΔC1

(Miller et al., 2017)

lz^UAS.cBa/Scer\GAL4^lz-gal4 is a suppressor of increased cell size | third instar larval stage phenotype of DnaJ-1^C/DnaJ-1^A

(Miller et al., 2017)

lz^UAS.cBa/Scer\GAL4^lz-gal4 is a suppressor of increased cell number | third instar larval stage phenotype of DnaJ-1^C/DnaJ-1^A

(Miller et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^e33C, lz^UAS.cBa has embryonic/larval crystal cell | ectopic phenotype, enhanceable by srp^NC.F.UAS/Scer\GAL4^e33C

(Fossett et al., 2003)

Scer\GAL4^twi.PB, lz^UAS.cBa has embryonic/larval crystal cell | ectopic phenotype, enhanceable by srp^NC.F.UAS/Scer\GAL4^twi.PB

(Fossett et al., 2003)

Enhancer of

Statement

Reference

Scer\GAL4^e33C/lz^UAS.cBa is an enhancer of embryonic/larval crystal cell | ectopic phenotype of Scer\GAL4^e33C, srp^NC.F.UAS

(Fossett et al., 2003)

lz^UAS.cBa/Scer\GAL4^twi.PB is an enhancer of embryonic/larval crystal cell | ectopic phenotype of Scer\GAL4^twi.PB, srp^NC.F.UAS

(Fossett et al., 2003)

Suppressor of

Statement

Reference

lz^UAS.cBa/Scer\GAL4^lz-gal4 is a suppressor of embryonic/larval crystal cell | third instar larval stage phenotype of Mlf^ΔC1

(Miller et al., 2017)

lz^UAS.cBa/Scer\GAL4^lz-gal4 is a suppressor of embryonic/larval crystal cell | third instar larval stage phenotype of DnaJ-1^C/DnaJ-1^A

(Miller et al., 2017)

lz^UAS.cBa/Scer\GAL4^lz-gal4 is a suppressor of embryonic/larval crystal cell | embryonic stage 15 phenotype of Mlf^Δ5-3

(Bras et al., 2012)

lz^UAS.cBa/Scer\GAL4^lz-gal4 is a suppressor of embryonic/larval crystal cell | third instar larval stage phenotype of Mlf^Δ5-3

(Bras et al., 2012)

lz^UAS.cBa, Scer\GAL4^lz-gal4 is a suppressor of embryonic/larval crystal cell phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cMa, Scer\GAL4^lz-gal4

(Osman et al., 2009)

lz^UAS.cBa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cMa, Scer\GAL4^GMR.PF

(Wildonger and Mann, 2005)

lz^UAS.cBa/Scer\GAL4^lz-gal4 is a suppressor of embryonic/larval crystal cell phenotype of Scer\GAL4^lz-gal4, srp^C.UAS

(Waltzer et al., 2003)

lz^UAS.cBa/Scer\GAL4^lz-gal4 is a suppressor of embryonic/larval crystal cell phenotype of Scer\GAL4^lz-gal4, srp^NC.UAS

(Waltzer et al., 2003)

NOT Suppressor of

Statement

Reference

lz^UAS.cBa/Scer\GAL4^twi.PG is a non-suppressor of embryonic/larval crystal cell phenotype of srp²

(Waltzer et al., 2003)

lz^UAS.cBa/Scer\GAL4^twi.PG is a non-suppressor of embryonic/larval crystal cell phenotype of srp³

(Waltzer et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The expression of lz^Scer\UAS.cBa under the control of Scer\GAL4^lz-gal4 suppresses the increased number and size of circulating crystal cells observed in DnaJ-1^A/DnaJ-1^C transheterozygous or Mlf^ΔC1 homozygous third instar larvae.

(Miller et al., 2017)

Expression of lz^Scer\UAS.cBa under the control of Scer\GAL4^lz-gal4 suppresses not only the drop in crystal cell number in Mlf^Δ5-3 mutant embryos, but also the rise in crystal cell number in Mlf^Δ5-3 larvae.

(Bras et al., 2012)

Expression of both lz^Scer\UAS.cBa and srp^{NC.F.Scer\UAS} when driven by Scer\GAL4^twi.PB enhances the ectopic crystal cell phenotype. Expression of both lz^Scer\UAS.cBa and srp^{NC.F.Scer\UAS} when driven by Scer\GAL4^e33C enhances the ectopic crystal cell phenotype.

(Fossett et al., 2003)

The increased expression of crystal cell markers in lz^Scer\UAS.cBa; Scer\GAL4^twi.PG embryos is enhanced and spreads throughout the mesoderm when srp^NC.Scer\UAS is present. This effect is not seen with srp^C.Scer\UAS. Expression of crystal cell markers (ectopic and endogenous) in lz^Scer\UAS.cBa; Scer\GAL4^twi.PG embryos, is suppressed by srp²/srp² or srp³/srp³.

(Waltzer et al., 2003)

Xenogenetic Interactions

Statement

Reference

Differentiation of crystal cells is significantly restored in larvae expressing Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cMa under the control of Scer\GAL4^lz-gal4 if they are also co-expressing lz^Scer\UAS.cBa.

(Osman et al., 2009)

Coexpression of lz^Scer\UAS.cBa suppresses the Scer\GAL4^GMR.PF>Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cMa eye phenotype.

(Wildonger and Mann, 2005)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

33836

w^*; P{UAS-lz.B}3

Notes on Origin

Discoverer

U. Banerjee

(Tracey et al., 2000)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

lz^Scer\UAS.cBa

lz^Scer\UAS.cSa

lz^UAS.cBa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Banerjee

(Tracey et al., 2000)

Secondary FlyBase IDs

FBal0103664
FBal0276922

References (19)

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