FlyBase Allele Report: Dmel\Apc2[33]

General Information

Symbol

Dmel\Apc2³³

Species

D. melanogaster

Name

FlyBase ID

FBal0213105

Feature type

allele

Associated gene

Dmel\Apc2

Associated Insertion(s)

P{?EPgy2}Apc2³³

Carried in Construct

Genomic Maps

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Allele class

hypomorphic allele - genetic evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Takacs et al., 2008)

Mutagen

Delta2-3 transposase

(Takacs et al., 2008)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

complex substitution

3R:24,163,537..24,165,274 [+]

Comment:

1738 bp deletion resulting from the imprecise excision of the P{EPgy2}EY09246. 720bp of the insertion remain at this deletion site. There is also a 4 bp deletion of bases 1313-1316 relative to the Apc2 transcription start site (R5.1).

(Takacs et al., 2008)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

P{EPgy2}EY09246

(Takacs et al., 2008)

Cytology

Nature of the lesion

Statement

Reference

Imprecise excision of the P{EPgy2}EY09246 insertion, resulting in a deletion of nucleotides -431 to +1307 relative to the Apc2 transcription start site. 720bp of the insertion remain at this deletion site. There is a second deletion of 4bp at positions +1313 to +1316.

(Takacs et al., 2008)

Insertion components

P{?EPgy2}Apc2³³

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of colorectal cancer

CEA with Apc^Q8

(Lee et al., 2009)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

denticle belt

(Takacs et al., 2008)

embryonic/first instar larval cuticle

(Takacs et al., 2008)

embryonic/larval cuticle | embryonic stage

(Roberts et al., 2012)

Detailed Description

Statement

Reference

Embryos maternally and zygotically mutant for Apc2³³ have an average cuticle score of 3.2 (based on the numerical scale described in McCartney et al., 1994, where 0 is a wild type embryo and 6 indicates the most severe defects).

(Roberts et al., 2012)

Severe hypomorph.

Homozygous embryos derived from homozygous females show an expansion of naked cuticle at the expense of denticles.

(Takacs et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Apc2³³, Apc^Q8, Axn^S044230 has abnormal neuroanatomy | somatic clone | third instar larval stage phenotype

(Roberts et al., 2012)

Apc2³³, Apc^Q8 has abnormal neuroanatomy | somatic clone | third instar larval stage phenotype

(Roberts et al., 2012)

Apc2³³/Apc2^5-3, Apc^Q8 has visible phenotype

(Takacs et al., 2008)

Apc2^G5028/Apc2³³, Apc^Q8 has visible phenotype

(Takacs et al., 2008)

Phenotype Manifest In

Suppressed by

Statement

Reference

Apc2³³, Apc^Q8 has intestinal stem cell | somatic clone phenotype, suppressible by pan^ΔN.UAS/Scer\GAL4^Tub.PU

(Lee et al., 2009)

Suppressor of

Statement

Reference

Apc2³³ is a suppressor of eye photoreceptor cell phenotype of Apc^Q8

(Takacs et al., 2008)

Apc2³³/Apc2³³ is a suppressor of eye photoreceptor cell phenotype of Scer\GAL4^elav-C155, arm^UAS.Exel

(Takacs et al., 2008)

Apc2[+]/Apc2³³ is a suppressor of eye photoreceptor cell phenotype of Apc^Q8

(Takacs et al., 2008)

Other

Statement

Reference

Apc2³³, Apc^Q8 has medulla | somatic clone | third instar larval stage phenotype

(Roberts et al., 2012)

Apc2³³, Apc^Q8, Axn^S044230 has medulla | somatic clone | third instar larval stage phenotype

(Roberts et al., 2012)

Apc2³³, Apc^Q8 has intestinal stem cell | somatic clone phenotype

(Lee et al., 2009)

Apc2^G5028/Apc2³³, Apc^Q8 has sternite phenotype

(Takacs et al., 2008)

Apc2³³/Apc2^5-3, Apc^Q8 has pleural membrane phenotype

(Takacs et al., 2008)

Apc2³³/Apc2^5-3, Apc^Q8 has abdominal sternite bristle | ectopic phenotype

(Takacs et al., 2008)

Apc2³³/Apc2^5-3, Apc^Q8 has sternite phenotype

(Takacs et al., 2008)

Apc2^G5028/Apc2³³, Apc^Q8 has pleural membrane phenotype

(Takacs et al., 2008)

Apc2^G5028/Apc2³³, Apc^Q8 has abdominal sternite bristle | ectopic phenotype

(Takacs et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

No apical constriction is seen in the cells surrounding the wing pouch in Apc^Q8 Apc2³³ double mutant third instar larval clones.

Apc2^19-3 Apc^Q8 double mutant clone cells generated in the medullar region of third instar larval brains sometimes segregate from their neighbours to form cysts. When clones are induced in the medullar neurons the axons extend correctly to the medullar neuropil.

(Roberts et al., 2012)

Apc2³³ Apc^Q8 double mutant intestinal stem cell clones in the adult midgut show hyperplasia.

The hyperplasia seen in Apc2³³ Apc^Q8 double mutant intestinal stem cell clones in the adult midgut is completely suppressed if they are also expressing pan^ΔN.Scer\UAS under the control of Scer\GAL4^tub.

(Lee et al., 2009)

The photoreceptor apoptosis phenotype seen in flies expressing arm^{Scer\UAS.Exel} under the control of Scer\GAL4^elav-C155 is partially suppressed by Apc2³³/Apc2³³.

Apc^Q8 Apc2^5-3/Apc^Q8 Apc2³³ double mutants show expansion of sternal territory in the abdomen and ectopic sternal bristles, at the expense of the pleura.

Apc^Q8 Apc2^G5028/Apc^Q8 Apc2³³ double mutants show expansion of sternal territory in the abdomen and ectopic sternal bristles, at the expense of the pleura.

(Takacs et al., 2008)

The photoreceptor cell apoptosis phenotype seen in Apc^Q8 homozygotes is suppressed by Apc2³³/+.

(Takacs et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

APC2³³

(Blundon et al., 2016, Roberts et al., 2012)

Apc2³³

(Wang et al., 2016, Benchabane et al., 2011, Lee et al., 2009, Takacs et al., 2008, Takacs et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (7)

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