FlyBase Allele Report: Dmel\Rab7[UASp.YFP]

General Information

Symbol

Dmel\Rab7^UASp.YFP

Species

D. melanogaster

Name

FlyBase ID

FBal0215398

Feature type

allele

Associated gene

Dmel\Rab7

Associated Insertion(s)

Carried in Construct

P{UASp-YFP.Rab7}

Also Known As

UAS-YFP-Rab7, YFP-Rab7, UAS-YFP::RAB7, UAS-RAB7-YFP, UASp-YFP-Rab7, UAS-Rab7, UAS-Rab7^WT, UAS-YFP:Rab7, Rab7-WT

Key Links

Transgenic product class

wild_type

(Zhang et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Zhang et al., 2007)

Mutagen

in vitro construct

(Zhang et al., 2007)

Progenitor genotype

Carried in construct

P{UASp-YFP.Rab7}

Cytology

Description

UASp regulatory sequences drive expression of wild-type Rab7 that is tagged at the N-terminal end with YFP.

(Zhang et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Rab7

Tagged with

YFP

yellow fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates C9orf72 frontotemporal dementia and/or amyotrophic lateral sclerosis

modeled by Zzzz\GGGGCC^30.UAS.EGFP

(Cunningham et al., 2020)

ameliorates motor neuron disease

modeled by scat^HMS01910

(Patel et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | larval stage, with Scer\GAL4^futsch-C380

(Patel et al., 2020)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Rab7^UASp.YFP, Scer\GAL4^futsch-C380 is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of Scer\GAL4^futsch-C380, scat^HMS01910

(Patel et al., 2020)

NOT Suppressor of

Statement

Reference

Rab7^UASp.YFP, Scer\GAL4^c306 is a non-suppressor of abnormal cell polarity | oogenesis phenotype of Scer\GAL4^c306, cactin^KK100507

(Miao et al., 2022)

Rab7^UASp.YFP, Scer\GAL4^c306 is a non-suppressor of abnormal cell migration | oogenesis phenotype of Scer\GAL4^c306, cactin^KK100507

(Miao et al., 2022)

Other

Statement

Reference

Rab7^UASp.YFP, Scer\GAL4^futsch-C380, scat^HMS01910 has abnormal locomotor behavior | larval stage phenotype

(Patel et al., 2020)

Phenotype Manifest In

Suppressor of

Statement

Reference

Rab7^UASp.YFP, Scer\GAL4^futsch-C380 is a suppressor | partially of embryonic/larval neuromuscular junction | larval stage phenotype of Scer\GAL4^futsch-C380, scat^HMS01910

(Patel et al., 2020)

Rab7^UASp.YFP, Scer\GAL4^futsch-C380 is a suppressor | partially of NMJ bouton | increased number | larval stage phenotype of Scer\GAL4^futsch-C380, scat^HMS01910

(Patel et al., 2020)

NOT Suppressor of

Statement

Reference

Rab7^UASp.YFP, Scer\GAL4^c306 is a non-suppressor of border follicle cell phenotype of Scer\GAL4^c306, cactin^KK100507

(Miao et al., 2022)

Scer\GAL4^dysf.C1.7/Rab7^UASp.YFP is a non-suppressor of tracheal dorsal trunk primordium | embryonic stage phenotype of stac^3B20

(Caviglia et al., 2016)

Scer\GAL4^btl.PU/Rab7^UASp.YFP is a non-suppressor of tracheal dorsal trunk primordium | embryonic stage phenotype of dnd¹

(Caviglia et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Rab7^{Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^btl.PU fails to rescue the tracheal fusion defects of dnd¹/dnd¹ mutant embryos.

Expression of Rab7^{Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^dysf.C1.7 fails to rescue the tracheal fusion defects of stac^3B20/stac^3B20 mutant embryos.

(Caviglia et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Rab7^UASp.YFP/Scer\GAL4^hs.PU rescues Df(3R)Exel6196/Rab7^d1

(Hegedűs et al., 2016)

Comments

Expression of Rab7^{Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^hs.PU rescues the loss of acidic autolysosomes (detected by LysoTracker staining) observed in fat cells of starved Rab7^d1/Df(3R)Exel6196 third instar larvae.

(Hegedűs et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

23641

y¹ w^*; P{UASp-YFP.Rab7}21/SM5

23270

y¹ w^*; P{UASp-YFP.Rab7}vig2¹⁸

Notes on Origin

Discoverer