FlyBase Allele Report: Dmel\Rab7[d1]

General Information

Symbol

Dmel\Rab7^d1

Species

D. melanogaster

Name

FlyBase ID

FBal0325096

Feature type

allele

Associated gene

Dmel\Rab7

Associated Insertion(s)

Carried in Construct

Also Known As

rab7¹

Key Links

Allele class

amorphic allele - genetic evidence

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Hegedűs et al., 2016)

amorphic allele - molecular evidence

(Hegedűs et al., 2016)

Mutagen

P-element activity

(Hegedűs et al., 2016)

Progenitor genotype

P{EPgy2}Rab7^EY10675

(Hegedűs et al., 2016)

Cytology

Description

1025bp deletion that removes most of the first protein-coding exon and 5'UTR of all three predicted Rab7 isoforms. Generated by imprecise excision of P{EPgy2}Rab7^EY10675.

(Hegedűs et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of P-stage

(Hegedűs et al., 2016)

Phenotype Manifest In

autolysosome | third instar larval stage (with Df(3R)Exel6196)

(Hegedűs et al., 2016)

autophagosome | third instar larval stage

(Hegedűs et al., 2016)

autophagosome | third instar larval stage (with Df(3R)Exel6196)

(Hegedűs et al., 2016)

embryonic/larval fat body | third instar larval stage

(Hegedűs et al., 2016)

embryonic/larval fat body | third instar larval stage (with Df(3R)Exel6196)

(Hegedűs et al., 2016)

Detailed Description

Statement

Reference

Rab7^d1 homozygous mutants die at late pupal stage. The number of autophagic structures is increased but their average size is decreased in fat cells of starved Rab7^d1 mutant third instar larvae.

The number of acidic autolysosomes (detected by LysoTracker staining) is strongly reduced in fat body cells of starved Rab7^d1/Df(3R)Exel6196 mutant third instar larvae relative to controls, while the cells show abnormal accumulation of autophagosomes.

(Hegedűs et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Other

Statement

Reference

Mon1^Δ181, Rab7^d1 has NMJ bouton | larval stage phenotype

(Basargekar et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Mon1^Δ181/+ Rab7^d1/+ larvae exhibit bigger and well-spaced synaptic boutons with fewer satellites compared to Mon1^Δ181 homozygotes.

(Basargekar et al., 2020)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Df(3R)Exel6196/Rab7^d1 is rescued by Rab7^UASp.YFP/Scer\GAL4^hs.PU

(Hegedűs et al., 2016)

Comments

Expression of Rab7^{Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^hs.PU rescues the loss of acidic autolysosomes (detected by LysoTracker staining) observed in fat cells of starved Rab7^d1/Df(3R)Exel6196 third instar larvae.

(Hegedűs et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Rab7¹

(Basargekar et al., 2020, Hegedűs et al., 2016)

Rab7^d1

(Krzystek et al., 2023, Hegedűs et al., 2016)

Rab7^Δ1

(Lund et al., 2021)

rab7¹

(Lőrincz et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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