FlyBase Allele Report: Dmel\Rab8[UASp.YFP]

General Information

Symbol

Dmel\Rab8^UASp.YFP

Species

D. melanogaster

Name

FlyBase ID

FBal0215401

Feature type

allele

Associated gene

Dmel\Rab8

Associated Insertion(s)

Carried in Construct

P{UASp-YFP.Rab8}

Also Known As

UASp-YFP-Rab8, UAS-Rab8-YFP

Key Links

Transgenic product class

wild_type

(Zhang et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Zhang et al., 2007)

Mutagen

in vitro construct

(Zhang et al., 2007)

Progenitor genotype

Carried in construct

P{UASp-YFP.Rab8}

Cytology

Description

UASp regulatory sequences drive expression of wild-type Rab8 that is tagged at the N-terminal end with YFP.

(Zhang et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Rab8

Tagged with

YFP

yellow fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by Hsap\SNCA^UAS.cKa

(Yin et al., 2014)

modeled by Hsap\SNCA^UAS.cUa

(Yin et al., 2014)

modeled by Hsap\SNCA^TP.UAS.cKa

(Yin et al., 2014)

ameliorates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Delfino et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Rab8^UASp.YFP, Scer\GAL4^elav-C155 is a non-enhancer of partially lethal - majority die phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Delfino et al., 2020)

Suppressor of

Statement

Reference

Rab8^UASp.YFP, Scer\GAL4^Pdf.PU is a suppressor | partially of abnormal locomotor rhythm phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Pdf.PU

(Delfino et al., 2020)

Rab8^UASp.YFP, Scer\GAL4^elav-C155 is a suppressor of short lived phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Delfino et al., 2020)

Rab8^UASp.YFP/Scer\GAL4^pnr.PU is a suppressor of abnormal cell number | somatic clone | P-stage phenotype of strat^CRISPR

(Bellec et al., 2018)

Rab8^UASp.YFP, Scer\GAL4^ple.PF is a suppressor of abnormal locomotor behavior | third instar larval stage phenotype of Hsap\SNCA^UAS.cUa, Scer\GAL4^ple.PF

(Yin et al., 2014)

Rab8^UASp.YFP, Scer\GAL4^C164 is a suppressor of abnormal locomotor behavior | third instar larval stage phenotype of Hsap\SNCA^UAS.cUa, Scer\GAL4^C164

(Yin et al., 2014)

Rab8^UASp.YFP, Scer\GAL4^elav.PU is a suppressor of abnormal locomotor behavior | adult stage phenotype of Hsap\SNCA^UAS.cUa, Scer\GAL4^elav.PU

(Yin et al., 2014)

Rab8^UASp.YFP, Scer\GAL4^C164 is a suppressor of abnormal locomotor behavior | third instar larval stage phenotype of Hsap\SNCA^TP.UAS.cKa, Scer\GAL4^C164

(Yin et al., 2014)

Rab8^UASp.YFP, Scer\GAL4^ple.PF is a suppressor of abnormal locomotor behavior | third instar larval stage phenotype of Hsap\SNCA^TP.UAS.cKa, Scer\GAL4^ple.PF

(Yin et al., 2014)

Rab8^UASp.YFP, Scer\GAL4^C164 is a suppressor of abnormal locomotor behavior | third instar larval stage phenotype of Hsap\SNCA^UAS.cKa, Scer\GAL4^C164

(Yin et al., 2014)

Rab8^UASp.YFP, Scer\GAL4^ple.PF is a suppressor of abnormal locomotor behavior | third instar larval stage phenotype of Hsap\SNCA^UAS.cKa, Scer\GAL4^ple.PF

(Yin et al., 2014)

NOT Suppressor of

Statement

Reference

Rab8^UASp.YFP, Scer\GAL4^elav-C155 is a non-suppressor of partially lethal - majority die phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Delfino et al., 2020)

Phenotype Manifest In

Suppressor of

Statement

Reference

Rab8^UASp.YFP, Scer\GAL4^elav-C155 is a suppressor of rhabdomere | decreased number phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Delfino et al., 2020)

Rab8^UASp.YFP, Scer\GAL4^elav-C155 is a suppressor of eye phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Delfino et al., 2020)

Rab8^UASp.YFP/Scer\GAL4^pnr.PU is a suppressor of sensory neuron | somatic clone | P-stage phenotype of strat^CRISPR

(Bellec et al., 2018)

NOT Suppressor of

Statement

Reference

Rab8^UASp.YFP, Scer\GAL4^tj.PU is a non-suppressor of follicle cell phenotype of Scer\GAL4^tj.PU, strat^GD10605

(Devergne et al., 2017)

Rab8^UASp.YFP, Scer\GAL4^tj.PU is a non-suppressor of ovarian basement membrane | oogenesis phenotype of Scer\GAL4^tj.PU, strat^GD10605

(Devergne et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of Rab8^{Scer\UAS.P\T.T:Avic\GFP-YFP} does not suppress the basement membrane deposition defects observed in follicle cells expressing strat^GD10605 under the control of Scer\GAL4^tj.PU.

(Devergne et al., 2017)

Xenogenetic Interactions

Statement

Reference

The locomotion defects characteristic for third instar larvae expressing Hsap\SNCA^Scer\UAS.cUa under the control of either Scer\GAL4^C164 or Scer\GAL4^ple.PF can be suppressed by co-expression of Rab8^{Scer\UAS.P\T.T:Avic\GFP-YFP} : the decrease in the distance crawled by third instar larvae per unit of time is significantly ameliorated.

The locomotion defects characteristic for third instar larvae expressing Hsap\SNCA^{TP.Scer\UAS.cKa} under the control of either Scer\GAL4^C164 or Scer\GAL4^ple.PF can be suppressed by co-expression of Rab8^{Scer\UAS.P\T.T:Avic\GFP-YFP} : the decrease in the distance crawled by third instar larvae per unit of time is significantly ameliorated.

The locomotion defects characteristic for third instar larvae expressing Hsap\SNCA^Scer\UAS.cKa under the control of either Scer\GAL4^C164 or Scer\GAL4^ple.PF can be suppressed by co-expression of Rab8^{Scer\UAS.P\T.T:Avic\GFP-YFP} : the decrease in the distance crawled by third instar larvae per unit of time is significantly ameliorated.

The negative geotaxis defects characteristic for flies expressing Hsap\SNCA^Scer\UAS.cUa under the control of either Scer\GAL4^elav.PU can be suppressed by co-expression of Rab8^{Scer\UAS.P\T.T:Avic\GFP-YFP} : the reduction in climbing activity of adult flies is significantly ameliorated.

The reduction in the number of dopaminergic neurons from the PPL1 clusters characteristic for adult flies expressing Hsap\SNCA^Scer\UAS.cKa under the control of either Scer\GAL4^elav.PU or Scer\GAL4^ple.PF can be fully rescued by co-expression with Rab8^{Scer\UAS.P\T.T:Avic\GFP-YFP}. The loss of dopaminergic neurons from the PPM3 cluster seen in adult flies expressing Hsap\SNCA^Scer\UAS.cKa under the control of Scer\GAL4^elav.PU is partially restored by co-expression with Rab8^{Scer\UAS.P\T.T:Avic\GFP-YFP}.