FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Hsap\SNCATP.UAS.cKa
Open Close
General Information
Symbol
Hsap\SNCATP.UAS.cKa
Species
H. sapiens
Name
FlyBase ID
FBal0269389
Feature type
allele
Associated gene
Hsap\SNCA
Associated Insertion(s)
Carried in Construct
M{UAS-αSyn.TP}
Key Links
Transgenic product class
missense_variant
(Karpinar et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Karpinar et al., 2009)
Mutagen
in vitro construct
(Karpinar et al., 2009)
Progenitor genotype
Carried in construct
M{UAS-αSyn.TP}
Cytology
Description

UASt regulatory sequences drive expression of a mutant form of Hsap\SNCA (amino acid replacements A30P, A56P and A76P).

(Karpinar et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Hsap\SNCA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      model of  Parkinson's disease
      is ameliorated by Rab8UASp.YFP
      (Yin et al., 2014)
      Comments on Models/Modifiers Based on Experimental Evidence ( 2 )
       

      Targeted expression of Hsap\SNCATP.Scer\UAS.cKa not only recapitulates Parkinson's disease motor symptoms but also the preceding non-motor symptoms such as an abnormal sleep-like behavior, altered locomotor activity and abnormal circadian periodicity.

      (Gajula Balija et al., 2011)

      Mutations in Hsap\SNCA (based on a structure-based design rationale) which increase neurotoxicity, show reduced fibril and β-structure formation and increased soluble oligomers in vitro.

      (Karpinar et al., 2009)
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Ectopic expression of Hsap\SNCATP.Scer\UAS.cKa under the control of either Scer\GAL4C164 or Scer\GAL4ple.PF results in defects in locomotion and a significant decrease in the distance crawled by third instar larvae per unit of time compared to controls.

      (Yin et al., 2014)

      Expression of Hsap\SNCATP.Scer\UAS.cKa in serotonergic and dopaminergic neurons, under the control of Scer\GAL4Ddc.PL, leads to a strong increase in both the number and length of sleep episodes respectively, when compared to controls in both light and dark phases. Expression of Hsap\SNCATP.Scer\UAS.cKa under the control of Scer\GAL4Ddc.PL leads to a significant reduction in the time the flies spend asleep during a 24 hour period.

      Expression of Hsap\SNCATP.Scer\UAS.cKa in serotonergic and dopaminergic neurons, under the control of Scer\GAL4Ddc.PL alters circadian locomotor activity. Anticipation behaviour is maintained in young (1-3 days after eclosion) flies expressing Hsap\SNCATP.Scer\UAS.cKa. Old flies (30 days after eclosion) lose this behaviour. Hsap\SNCATP.Scer\UAS.cKa expression causes a phasing out of locomotion activity at the light-dark transition. This effect is further enhanced in older flies.

      Young flies expressing Hsap\SNCATP.Scer\UAS.cKa under the control of Scer\GAL4Ddc.PL display wild-type circardian periodicity. Old flies shift their periodicity in a similar way to per mutants.

      Expression of Hsap\SNCATP.Scer\UAS.cKa in DA neurons under the control of Scer\GAL4ple.PF causes a phase shift in circadian locomotor activity and gradually extends the circadian periodicity.

      (Gajula Balija et al., 2011)

      The expression of Hsap\SNCATP.Scer\UAS.cKa under the control of Scer\GAL4Ddc.PL results in significant decreases in adult lifespan, as compared to controls.

      (Karpinar et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      The locomotion defects characteristic for third instar larvae expressing Hsap\SNCATP.Scer\UAS.cKa under the control of either Scer\GAL4C164 or Scer\GAL4ple.PF can be suppressed by co-expression of Rab8Scer\UAS.P\T.T:Avic\GFP-YFP : the decrease in the distance crawled by third instar larvae per unit of time is significantly ameliorated.

      (Yin et al., 2014)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsap\SNCATP.Scer\UAS.cKa
      Hsap\SNCATP.UAS.cKa
      Name Synonyms
      Secondary FlyBase IDs
        References (3)