FlyBase Allele Report: Dmel\PDZ-GEF[6]

General Information

Symbol

Dmel\PDZ-GEF⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0216664

Feature type

allele

Associated gene

Dmel\PDZ-GEF

Associated Insertion(s)

Carried in Construct

Also Known As

Gef26⁶

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Singh et al., 2006, Wang et al., 2006)

Mutagen

P-element activity

(Wang et al., 2006)

Progenitor genotype

PDZ-GEF³

(Wang et al., 2006)

Cytology

Description

Imprecise excision of P{lacW}Gef26³ has produced a 3 kb deletion surrounding the insertion site including the first 2 exons of Gef26 and a large 5' chunk of the third.

(Wang et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | spermatogenesis (with PDZ-GEF⁴)

(Wang et al., 2006)

partially lethal (with PDZ-GEF⁴)

(Singh et al., 2006)

sterile (with PDZ-GEF⁴)

(Wang et al., 2006)

visible (with PDZ-GEF⁴)

(Singh et al., 2006)

Phenotype Manifest In

embryonic/larval neuromuscular junction | larval stage (with Df(2L)BSC5)

(Kim et al., 2024)

eye (with PDZ-GEF⁴)

(Singh et al., 2006)

macropinosome | larval stage (with Df(2L)BSC5)

(Kim et al., 2024)

male germline stem cell (with PDZ-GEF⁴)

(Wang et al., 2006)

NMJ bouton | increased number | larval stage (with Df(2L)BSC5)

(Kim et al., 2024)

spermatheca | increased number (with PDZ-GEF⁴)

(Singh et al., 2006)

wing (with PDZ-GEF⁴)

(Singh et al., 2006)

Detailed Description

Statement

Reference

Eye and wing phenotypes (small rough eyes, abnormal wings) and some degree of lethality are seen in various PDZ-GEF allelic combinations, with their relative strength in the following order: PDZ-GEF⁴/PDZ-GEF⁶ = PDZ-GEF⁴/PDZ-GEF⁵ > PDZ-GEF³/PDZ-GEF⁴ > PDZ-GEF^k13720/PDZ-GEF³ = PDZ-GEF^k13720/PDZ-GEF⁴ > PDZ-GEF^EP388/PDZ-GEF³ > PDZ-GEF^k13720/PDZ-GEF^k13720 > PDZ-GEF^k13720/PDZ-GEF^EP388 > PDZ-GEF^EP388/PDZ-GEF^EP388.

Many mutant females have three spermathecae instead of two, with different allelic combinations having different percentages of females with three spermathecae: PDZ-GEF³/PDZ-GEF³ 22%, PDZ-GEF³/PDZ-GEF⁴ 30%, PDZ-GEF⁴/PDZ-GEF⁵ 59%, PDZ-GEF⁴/PDZ-GEF⁶ 62%, PDZ-GEF^k13720/PDZ-GEF⁴ % (all PDZ-GEF^k13720/PDZ-GEF⁴, PDZ-GEF^k13720/PDZ-GEF³ or PDZ-GEF^EP388/PDZ-GEF³ females have two spermathecae).

(Singh et al., 2006)

The number of male germline stem cells is dramatically reduced in the testes of Gef26⁶/Gef26⁴ animals compared to wild-type. In these testes, the secondary spermatogonia, with branched fusomes, move to the tip.

Gef26⁶ homozygous germline stem cell clones develop normally.

(Wang et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Df(2L)BSC5/PDZ-GEF⁶ has NMJ bouton | larval stage | increased number phenotype, non-enhanceable by Scer\GAL4^elav-C155/Abl^UAS.cFa

(Kim et al., 2024)

Suppressed by

Statement

Reference

PDZ-GEF⁶/PDZ-GEF⁴ has cell-cell adherens junction & hub cell phenotype, suppressible by Rap1^{V12.UAS.Tag:MYC}/Scer\GAL4^upd1.PU

(Wang et al., 2006)

NOT suppressed by

Statement

Reference

Df(2L)BSC5/PDZ-GEF⁶ has NMJ bouton | increased number | larval stage phenotype, non-suppressible by Scer\GAL4^elav-C155/Abl^UAS.cFa

(Kim et al., 2024)

Suppressor of

Statement

Reference

PDZ-GEF[+]/PDZ-GEF⁶ is a suppressor of NMJ bouton | larval stage | decreased number phenotype of Abl^UAS.cFa, Scer\GAL4^elav-C155

(Kim et al., 2024)

Other

Statement

Reference

Abl⁴, PDZ-GEF⁶ has embryonic/larval neuromuscular junction | larval stage phenotype

(Kim et al., 2024)

Abl⁴, PDZ-GEF⁶ has NMJ bouton | increased number | larval stage phenotype

(Kim et al., 2024)

Abl⁴, PDZ-GEF⁶ has macropinosome | larval stage phenotype

(Kim et al., 2024)

PDZ-GEF⁶, Rap1^rvB1 has embryonic/larval neuromuscular junction | larval stage phenotype

(Kim et al., 2024)

PDZ-GEF⁶, Rap1^rvB1 has NMJ bouton | increased number | larval stage phenotype

(Kim et al., 2024)

PDZ-GEF⁶, Rap1^rvB1 has macropinosome | larval stage phenotype

(Kim et al., 2024)

Additional Comments

Genetic Interactions

Statement

Reference

R^{V12.Scer\UAS.T:Hsap\MYC}; Scer\GAL4^upd rescues the formation of adherens junctions between male germline stem cells and hub cells in the germinal proliferation center of Gef26⁴/Gef26⁶ animals.

(Wang et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

PDZ-GEF^+t11.6 rescues eye and wing phenotypes of PDZ-GEF⁴/PDZ-GEF⁶ flies.

(Singh et al., 2006)

Germline stem cell loss in the testes of Gef26⁴/Gef26⁶ animals is rescued by Gef26^+t11.6.

(Wang et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Gef26⁶

(Michel et al., 2011, Singh et al., 2006)

Gef26^Δ6

(Kim et al., 2024)

PDZ-GEF⁶

(Sawant et al., 2018)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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