FlyBase Allele Report: Dmel\Septin4[UAS.cMa]

General Information

Symbol

Dmel\Septin4^UAS.cMa

Species

D. melanogaster

Name

FlyBase ID

FBal0217550

Feature type

allele

Associated gene

Dmel\Septin4

Associated Insertion(s)

Carried in Construct

P{UAS-Septin4.cMa}

Also Known As

UAS-Sep4

Key Links

Transgenic product class

cDNA

(Muñoz-Soriano and Paricio, 2007)

wild_type

(Muñoz-Soriano and Paricio, 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Muñoz-Soriano and Paricio, 2007)

wild_type

(Muñoz-Soriano and Paricio, 2007)

Mutagen

in vitro construct

(Muñoz-Soriano and Paricio, 2007)

Progenitor genotype

Carried in construct

P{UAS-Septin4.cMa}

Cytology

Description

UASt regulatory sequences drive expression of the LP06017 Sep4 cDNA.

(Muñoz-Soriano and Paricio, 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Septin4

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA

(Muñoz-Soriano and Paricio, 2007)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of neurodegenerative disease

is ameliorated by park^UAS.cMa

(Muñoz-Soriano and Paricio, 2007)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^Ddc.PL

(Muñoz-Soriano and Paricio, 2007)

abnormal neuroanatomy | adult stage, with Scer\GAL4^ple.PF

(Muñoz-Soriano and Paricio, 2007)

visible | adult stage, with Scer\GAL4^da.G32

(Muñoz-Soriano and Paricio, 2007)

Phenotype Manifest In

dopaminergic neuron & adult brain, with Scer\GAL4^Ddc.PL

(Muñoz-Soriano and Paricio, 2007)

dopaminergic neuron & adult brain, with Scer\GAL4^ple.PF

(Muñoz-Soriano and Paricio, 2007)

eye, with Scer\GAL4^GMR.PF

(Muñoz-Soriano and Paricio, 2007)

wing, with Scer\GAL4^da.G32

(Muñoz-Soriano and Paricio, 2007)

Detailed Description

Statement

Reference

Expression of Sep4^Scer\UAS.cMa under the control of Scer\GAL4^Ddc.PL or Scer\GAL4^ple.PF results in a significant reduction in the number of dopaminergic (DA) neurons in the dorsomedial cluster of the brain in 40-day old flies. In contrast, the number of DA neurons in other clusters, like PPL1, PPL2, PPM3, VUM and PAL, are not reduced in these experiments.

Expression of Sep4^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PF results in very mild rough eyes, though such eyes have a normal complement and morphology of photoreceptors.

Scer\GAL4^da.G32-driven expression of two copies of Sep4^Scer\UAS.cMa results in obvious wing phenotypes, ranging from slightly curved to severely wrinkled wings. (No phenotype is observed when one copy of Sep4^Scer\UAS.cMa is used.)

(Muñoz-Soriano and Paricio, 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^Ddc.PL, Septin4^UAS.cMa has abnormal neuroanatomy | adult stage phenotype, enhanceable by park^Δ21/park[+]

(Muñoz-Soriano and Paricio, 2007)

Scer\GAL4^da.G32, Septin4^UAS.cMa has visible | adult stage phenotype, enhanceable by park^Δ21/park[+]

(Muñoz-Soriano and Paricio, 2007)

Scer\GAL4^da.G32, Septin4^UAS.cMa has visible | adult stage phenotype, enhanceable by Df(3L)Pc-MK/+

(Muñoz-Soriano and Paricio, 2007)

Suppressed by

Statement

Reference

Scer\GAL4^Ddc.PL, Septin4^UAS.cMa has abnormal neuroanatomy | adult stage phenotype, suppressible | partially by park^UAS.cMa/Scer\GAL4^Ddc.PL

(Muñoz-Soriano and Paricio, 2007)

Scer\GAL4^ple.PF, Septin4^UAS.cMa has abnormal neuroanatomy | adult stage phenotype, suppressible | partially by Scer\GAL4^ple.PF/park^UAS.cMa

(Muñoz-Soriano and Paricio, 2007)

Other

Statement

Reference

Df(3L)Pc-MK/+, Scer\GAL4^da.G32, Septin4^UAS.cMa has partially lethal - majority die phenotype

(Muñoz-Soriano and Paricio, 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^da.G32, Septin4^UAS.cMa has wing phenotype, enhanceable by Df(3L)GN34/+

(Munoz-Soriano et al., 2012)

Scer\GAL4^da.G32, Septin4^UAS.cMa has wing phenotype, enhanceable by Df(3L)81k19/+

(Munoz-Soriano et al., 2012)

Scer\GAL4^da.G32, Septin4^UAS.cMa has wing phenotype, enhanceable by Df(3L)BSC8/+

(Munoz-Soriano et al., 2012)

Scer\GAL4^da.G32, Septin4^UAS.cMa has wing phenotype, enhanceable by Df(3L)ri-XT1/+

(Munoz-Soriano et al., 2012)

Scer\GAL4^da.G32, Septin4^UAS.cMa has wing phenotype, enhanceable by Df(3R)T-32/+

(Munoz-Soriano et al., 2012)

Scer\GAL4^da.G32, Septin4^UAS.cMa has wing phenotype, enhanceable by Df(3R)DG2/+

(Munoz-Soriano et al., 2012)

Scer\GAL4^da.G32, Septin4^UAS.cMa has wing phenotype, enhanceable by Nedd4[+]/Nedd4^T119FS

(Munoz-Soriano et al., 2012)

Scer\GAL4^da.G32, Septin4^UAS.cMa has wing phenotype, enhanceable by +/Df(2L)XE-2750

(Munoz-Soriano et al., 2012)

Scer\GAL4^Ddc.PL, Septin4^UAS.cMa has dopaminergic neuron & adult brain phenotype, enhanceable by park^Δ21/park[+]

(Muñoz-Soriano and Paricio, 2007)

Scer\GAL4^da.G32, Septin4^UAS.cMa has wing phenotype, enhanceable by park^Δ21/park[+]

(Muñoz-Soriano and Paricio, 2007)

Scer\GAL4^da.G32, Septin4^UAS.cMa has wing phenotype, enhanceable by Df(3L)Pc-MK/+

(Muñoz-Soriano and Paricio, 2007)

Scer\GAL4^GMR.PF, Septin4^UAS.cMa has eye phenotype, enhanceable by park^Δ21/park[+]

(Muñoz-Soriano and Paricio, 2007)

Scer\GAL4^GMR.PF, Septin4^UAS.cMa has eye phenotype, enhanceable by Df(3L)Pc-MK/+

(Muñoz-Soriano and Paricio, 2007)

Scer\GAL4^GMR.PF, Septin4^UAS.cMa has eye phenotype, enhanceable by Df(3L)Pc-MK/park^Δ21

(Muñoz-Soriano and Paricio, 2007)

NOT Enhanced by

Statement

Reference

Scer\GAL4^da.G32, Septin4^UAS.cMa has wing phenotype, non-enhanceable by Ubi-p63E[+]/Ubi-p63E^EY07341

(Munoz-Soriano et al., 2012)

Scer\GAL4^da.G32, Septin4^UAS.cMa has wing phenotype, non-enhanceable by Rop^G27/Rop[+]

(Munoz-Soriano et al., 2012)

Scer\GAL4^da.G32, Septin4^UAS.cMa has wing phenotype, non-enhanceable by Det[+]/Det^e01527

(Munoz-Soriano et al., 2012)

Scer\GAL4^da.G32, Septin4^UAS.cMa has wing phenotype, non-enhanceable by CG5823[+]/CG5823^f04935

(Munoz-Soriano et al., 2012)

Scer\GAL4^da.G32, Septin4^UAS.cMa has wing phenotype, non-enhanceable by CG5823[+]/CG5823^EY15491

(Munoz-Soriano et al., 2012)

Scer\GAL4^da.G32, Septin4^UAS.cMa has wing phenotype, non-enhanceable by Rim[+]/Rim^EY12862

(Munoz-Soriano et al., 2012)

Scer\GAL4^da.G32, Septin4^UAS.cMa has wing phenotype, non-enhanceable by Rim[+]/Rim^EY05246

(Munoz-Soriano et al., 2012)

Scer\GAL4^da.G32, Septin4^UAS.cMa has wing phenotype, non-enhanceable by tzn^KG07968/CG4365[+]

(Munoz-Soriano et al., 2012)

Scer\GAL4^da.G32, Septin4^UAS.cMa has wing phenotype, non-enhanceable by CG5873[+]/cysu^c00427

(Munoz-Soriano et al., 2012)

Suppressed by

Statement

Reference

Scer\GAL4^Ddc.PL, Septin4^UAS.cMa has dopaminergic neuron & adult brain phenotype, suppressible | partially by park^UAS.cMa/Scer\GAL4^Ddc.PL

(Muñoz-Soriano and Paricio, 2007)

Scer\GAL4^ple.PF, Septin4^UAS.cMa has dopaminergic neuron & adult brain phenotype, suppressible | partially by Scer\GAL4^ple.PF/park^UAS.cMa

(Muñoz-Soriano and Paricio, 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The penetrance of the wing phenotype caused by expression of two copies of Sep4^Scer\UAS.cMa under the control of Scer\GAL4^da.G32 is enhanced if the flies are also heterozygous for one of Df(2L)XE-2750, Df(3L)GN34, Df(3L)81k19, Df(3L)BSC8, Df(3L)ri-XT1, Df(3R)T-32 or Df(3R)DG2.

The penetrance of the wing phenotype caused by expression of two copies of Sep4^Scer\UAS.cMa under the control of Scer\GAL4^da.G32 is not enhanced if the flies are also heterozygous for one of Ubi-p63E^EY07341, Rop^G27, tzn^KG07968, cysu^c00427, Det^e01527, CG5823^f04935, CG5823^EY15491, Rim^EY12862 or Rim^EY05246.

Heterozygosity for Nedd4^T119FS enhances the wing phenotype caused by expression of two copies of Sep4^Scer\UAS.cMa under the control of Scer\GAL4^da.G32.

(Munoz-Soriano et al., 2012)

The reduction in the number of dopaminergic neurons in the dorsomedial clusters of 40-day old adult brains caused by expression of Sep4^Scer\UAS.cMa under the control of Scer\GAL4^Ddc.PL or Scer\GAL4^ple.PF is almost restored back to control levels when of park^Scer\UAS.cMa is coexpressed.

Heterozygosity for park^Δ21 enhances the loss of dopaminergic neurons in the dorsomedial clusters of 40-day old adult brains caused by expression of Sep4^Scer\UAS.cMa under the control of Scer\GAL4^Ddc.PL.

Heterozygosity for park^Δ21 increases the percentage of Sep4^Scer\UAS.cMa, Scer\GAL4^da.G32 flies showing a wrinkled wing phenotype.

Heterozygosity for Df(3L)Pc-MK, which uncovers park, causes partial lethality to flies expressing Sep4^Scer\UAS.cMa under the control of Scer\GAL4^da.G32, but the percentage of escapers showing wrinkled wings is increased compared to Sep4^Scer\UAS.cMa, Scer\GAL4^da.G32 flies.

The mild rough eye phenotype seen in flies expressing Sep4^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PF is enhanced in flies heterozygous for either park^Δ21 or Df(3L)Pc-MK, and is further enhanced in park^Δ21/Df(3L)Pc-MK trans-heterozygous flies.

(Muñoz-Soriano and Paricio, 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Sep4^Scer\UAS.cMa

Sep4^UAS.cMa

Septin4^UAS.cMa

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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