FlyBase Allele Report: Dmel\Dg[O86]

General Information

Symbol

Dmel\Dg^O86

Species

D. melanogaster

Name

FlyBase ID

FBal0218114

Feature type

allele

Associated gene

Dmel\Dg

Associated Insertion(s)

Carried in Construct

Also Known As

Dg⁰⁸⁶

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Christoforou et al., 2008)

Progenitor genotype

Cytology

Description

Nucleotide substitution: C880T.

(Christoforou et al., 2008)

Amino acid replacement: R87term.

(Christoforou et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:16,089,139..16,089,139 [-]

Nucleotide change:

C16089139T

Reported nucleotide change:

C880T

Amino acid change:

R87term | Dg-PA; R87term | Dg-PB; R87term | Dg-PC; R87term | Dg-PD

Reported amino acid change:

R87term

Comment:

Reported coordinates appear to be relative to GB:GH09323.

(Christoforou et al., 2008)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of muscular dystrophy

CEA with Dg^O55

(Carney et al., 2023, Yatsenko et al., 2021, Marrone et al., 2012, Kucherenko et al., 2011)

CEA

(Yatsenko et al., 2021, Kucherenko et al., 2011, Marrone et al., 2011)

model of muscular dystrophy-dystroglycanopathy

CEA with Dg²⁴⁸

(Monagas-Valentin et al., 2023)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of muscular dystrophy

is exacerbated by Sec10^f03085

(Yatsenko et al., 2021)

is exacerbated by Sec15¹

(Yatsenko et al., 2021)

is exacerbated by Sec5^E10

(Yatsenko et al., 2021)

is exacerbated by Sec6^Ex15

(Yatsenko et al., 2021)

is exacerbated by Camⁿ³³⁹

(Marrone et al., 2011)

is exacerbated by Lis-1^k13209

(Kucherenko et al., 2011, Marrone et al., 2011)

is exacerbated by capt^E636

(Marrone et al., 2011)

is ameliorated by chif^BG02820a

(Marrone et al., 2011)

is exacerbated by dysc^GD14018

(Marrone et al., 2011)

is ameliorated by l(2)k09848^EMS-Mod4

(Marrone et al., 2011)

is exacerbated by Nrk^k14301

(Kucherenko et al., 2011)

is exacerbated by chif^BG02820a

(Kucherenko et al., 2011)

is exacerbated by chif^EY05746

(Kucherenko et al., 2011)

is exacerbated by Lis-1^k11702

(Kucherenko et al., 2011)

is exacerbated by Fhos^EY09842

(Kucherenko et al., 2011)

is exacerbated by Itgbn^BG01037

(Kucherenko et al., 2011)

is exacerbated by l(2)k09848^EMS-Mod4

(Kucherenko et al., 2011)

is exacerbated by Rack1^1.8

(Kucherenko et al., 2011)

is exacerbated by mbl^E27

(Kucherenko et al., 2011)

is exacerbated by Rack1^EE

(Kucherenko et al., 2011)

is exacerbated by dysc^c03838

(Kucherenko et al., 2011)

is exacerbated by uif^2B7

(Kucherenko et al., 2011)

is exacerbated by SP2353^MB00605

(Kucherenko et al., 2011)

is exacerbated by uif¹

(Kucherenko et al., 2011)

is exacerbated by Pgk^KG07478

(Kucherenko et al., 2011)

is exacerbated by vimar⁰⁹

(Kucherenko et al., 2011)

is exacerbated by Fkbp14⁰⁰⁷³⁴

(Kucherenko et al., 2011)

exacerbates muscular dystrophy-dystroglycanopathy

modeled by rt², rt^EP571

(Monagas-Valentin et al., 2023)

modeled by tw¹

(Monagas-Valentin et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

FlyBase curator comment: Interactions with Dg[086] and/or Dg[323] were detected in a Dg[086]/+ or Dg[323]/+ background which exhibits no obvious changes in muscle morphology. Nonetheless, these interactions have been captured as 'modifier' ('exacerbates') annotations here to best capture the experimental finding and the authors' intention.

(Kucherenko et al., 2011)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | dominant | temperature conditional

(Kucherenko et al., 2011)

abnormal locomotor behavior | temperature conditional (with Df(3R)Exel6184)

(Kucherenko et al., 2011)

abnormal locomotor behavior | temperature conditional (with Dg^O55)

(Kucherenko et al., 2011)

abnormal neuroanatomy

(Yatsenko et al., 2014)

abnormal neuroanatomy | somatic clone

(Yatsenko et al., 2014)

cell lethal | P-stage | somatic clone

(Walther et al., 2024)

decreased occurrence of cell division | larval stage

(Yatsenko et al., 2014)

decreased occurrence of cell division | larval stage | somatic clone

(Yatsenko et al., 2014)

partially lethal - majority die (with Dg^O55)

(Yatsenko et al., 2014)

partially lethal - majority live

(Christoforou et al., 2008)

partially lethal - majority live (with Df(2R)ED2457)

(Christoforou et al., 2008)

partially lethal - majority live (with Dg^J43)

(Christoforou et al., 2008)

viable (with Dg²⁴⁸)

(Mirouse et al., 2009)

viable (with Dg³²³)

(Mirouse et al., 2009)

viable (with Dg^O38)

(Mirouse et al., 2009)

viable (with Dg^O43)

(Mirouse et al., 2009)

viable (with Dg^O55)

(Mirouse et al., 2009)

visible

(Mirouse et al., 2009)

visible (with Dg²⁴⁸)

(Mirouse et al., 2009)

visible (with Dg³²³)

(Mirouse et al., 2009)

visible (with Dg^J43)

(Christoforou et al., 2008)

Phenotype Manifest In

actin filament | pupal stage | somatic clone

(Marrone et al., 2011)

brain

(Yatsenko et al., 2014)

brain | somatic clone

(Yatsenko et al., 2014)

crossvein

(Mirouse et al., 2009)

crossvein (with Dg²⁴⁸)

(Mirouse et al., 2009)

crossvein (with Dg³²³)

(Mirouse et al., 2009)

crossvein (with Dg^J43)

(Christoforou et al., 2008)

egg | maternal effect

(Mirouse et al., 2009)

follicle cell (with Dg^O43)

(Mirouse et al., 2009)

follicle cell | cell autonomous | somatic clone

(Mirouse et al., 2009)

hemocyte | pupal stage

(Christoforou et al., 2008)

indirect flight muscle cell

(Kucherenko et al., 2011)

lamina plexus | third instar larval stage

(Marrone et al., 2011)

lamina plexus | third instar larval stage (with Dg^O55)

(Marrone et al., 2011)

larval brain

(Yatsenko et al., 2014)

larval brain | somatic clone

(Yatsenko et al., 2014)

neuroblast

(Yatsenko et al., 2014)

neuroblast | somatic clone

(Yatsenko et al., 2014)

nucleus | pupal stage | somatic clone

(Marrone et al., 2011)

ommatidium | pupal stage | somatic clone

(Marrone et al., 2011)

oocyte (with Dg^O43)

(Kucherenko et al., 2008)

oocyte (with Dg^O55)

(Kucherenko et al., 2008)

retina | P-stage | somatic clone

(Walther et al., 2024)

rhabdomere | adult stage (with Dg^O55)

(Marrone et al., 2011)

stress fiber (with Dg^O43)

(Mirouse et al., 2009)

stress fiber | cell autonomous | somatic clone

(Mirouse et al., 2009)

Detailed Description

Statement

Reference

Dg^O55/Dg^O86 show no significant changes in systolic diameter, diastolic diameter or fractional shortening compared to wild type.

(Taghli-Lamallem et al., 2014)

Induction of Dg^O86 mutant clones causes abnormal clustering between clonal and non-clonal cells, resulting in altered 'cobblestone-like' brain organisation.

Dg^O86 mutant larval brains contain fewer actively dividing S-phase neuronal stem cells per lobe compare to controls. There are also fewer M-phase neuroblasts per lobe than in controls and this is also seen in Dg^O86 mutant clones.

(Yatsenko et al., 2014)

Dg^O86 follicle cell clones show normal polarity under both well-fed and starvation conditions. Polarity is also maintained after feeding flies drugs that reduce cellular ATP levels and activate AMPK, though many egg chambers degenerate at stage 7/8.

(Haack et al., 2013)

Homozygous Dg^O86 mutant flies exhibit indirect flight muscle degeneration.

Dg^O86/+ mutant flies exhibit temperature-induced mobility defects.

Dg^O86/Dg^O55 mutant flies exhibit temperature-induced mobility defects.

Dg^O86/Df(3R)Exel6184 mutant flies exhibit temperature-induced mobility defects.

Increased indirect flight muscle degeneration is seen in Dg^O86/Dg^O55 mutant flies at 25[o]C and, as in wild type, the phenotype is increased ~2 fold by an increase in temperature to 33[o]C. On paraquat-containing food the ratio or degree of muscle degeneration is not increased in Dg^O86/Dg^O55 mutant flies in comparison to control. Unlike in wild type, animals exposure of Dg^O86/Dg^O55 mutants to lower temperature (18[o]C) causes the appearance of severe progressive degeneration, followed by focal muscle loss. At 25[o]C the phenotype does not become significantly more severe with age. Sugar-free food conditions promote severe muscle loss. Metabolic rate on sugar-free food is reduced compared to wild type controls.

(Kucherenko et al., 2011)

Dg^O86 homozygous as well as Dg^O86/Dg^O55 transheterozygous third instar larvae frequently show lamina plexus defects in the brain. Adult Dg^O86/Dg^O55 transheterozygotes also display significantly reduced rhabdomere length compared to wild-type.

Dg^O86 homozygous somatic clones in the pupal retina have shorter ommatidia and display nuclei layering defects as well as aberrant actin distribution compared to wild-type cells.

(Marrone et al., 2011)

More than 80% of Dg^O86 flies have a "detached" crossvein phenotype, where the crossvein is gapped from both veins L4 and L5, while most of the remaining mutant flies have a "gapped" phenotype where there is a gap between the crossvein and either vein L4 or L5.

Approximately 60% of Dg^O86/Dg²⁴⁸ flies have a "detached" crossvein phenotype, where the crossvein is gapped from both veins L4 and L5, while most of the remaining mutant flies have a "gapped" phenotype where there is a gap between the crossvein and either vein L4 or L5.

Approximately 20% of Dg^O86/Dg³²³ flies have a "detached" crossvein phenotype, where the crossvein is gapped from both veins L4 and L5, approximately 60% have a "gapped" phenotype where there is a gap between the crossvein and either vein L4 or L5 and the remainder if the flies have a complete crossvein as in wild type.

The Dg[-] progeny of Dg^O38/Dg^O86 females mated to Dg^O43/+ males are viable when grown without competition from heterozygous larvae.

The Dg[-] progeny of Dg^O43/Dg^O86 females mated to Dg^O55/+ males are viable when grown without competition from heterozygous larvae.

The Dg[-] progeny of Dg^O55/Dg^O86 females mated to Dg^O38/+ males are viable when grown without competition from heterozygous larvae.

Dg^O86/Dg^O38, Dg^O86/Dg^O43 and Dg^O86/Dg^O55 animals show reduced viability under normal culture conditions.

73% of eggs laid by Dg^O38/Dg^O86 females mated to wild-type males hatch.

67% of eggs laid by Dg^O43/Dg^O86 females mated to wild-type males hatch.

66% of eggs laid by Dg^O55/Dg^O86 females mated to wild-type males hatch.

85% of eggs laid by Dg^O38/Dg^O86 females mated to Dg^O43/+ males hatch.

79% of eggs laid by Dg^O43/Dg^O86 females mated to Dg^O55/+ males hatch.

82% of eggs laid by Dg^O55/Dg^O86 females mated to Dg^O38/+ males hatch.

89% of eggs laid by Dg²⁴⁸/Dg^O86 females mated to wild-type males hatch.

83% of eggs laid by Dg³²³/Dg^O86 females mated to wild-type males hatch.

(Mirouse et al., 2009)

Females carrying homozygous germline clones produce eggs that are normal in shape.

Eggs laid by homozygous females are shorter and rounder than wild type.

Dg^O43/Dg^O86 follicle cells show a defect in the organisation of the actin stress fibres; the fibres are still properly aligned in mutant follicle cells, but they do not align along the dorsal-ventral axis, and they sometimes show a mixed orientation within a single cell.

The stress fibres are randomly oriented in homozygous follicle cell clones. This phenotype is cell autonomous.

Under normal food conditions, Dg^O86 mutant follicle cell clones have normal apical-basal polarity.

(Mirouse et al., 2009)

Homozygotes show a posterior crossvein phenotype with 100% penetrance. More than 80% of wings show a "detached" phenotype (the crossvein is detached from both L4 and L5). A fraction of wings with a "gapped" phenotype (the crossvein is detached from either vein L4 or L5, but not both) or a "point" phenotype (the crossvein is reduced to a point) are seen.

Dg^O86/Df(2R)ED2457 animals show 77% viability compared to controls.

The number of hemocytes in the posterior crossvein region in homozygous pupal wings is either greatly reduced compared to wild type or they are completely absent.

(Christoforou et al., 2008)

Dg^O86/Dg^O55 mutants are arrested at early stages of oogenesis and show mislocalisation of the oocyte.

Dg^O86/Dg^O43 mutants are arrested at early stages of oogenesis and show mislocalisation of the oocyte.

(Kucherenko et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Dg[+]/Dg^O86, Pgk^KG07478 has abnormal locomotor behavior | temperature conditional phenotype

(Kucherenko et al., 2011)

Dg^O86, dysc^c05107/dysc[+] has abnormal neuroanatomy | third instar larval stage phenotype

(Marrone et al., 2011)

Cam[+]/Camⁿ³³⁹, Dg^O86 has abnormal neuroanatomy | third instar larval stage phenotype

(Marrone et al., 2011)

Dg^O86, Lis-1[+]/Lis-1^k13209 has abnormal neuroanatomy | third instar larval stage phenotype

(Marrone et al., 2011)

Dg^O86, chif[+]/chif^BG02820a has abnormal neuroanatomy | third instar larval stage phenotype

(Marrone et al., 2011)

Df(3R)Exel6184/+, Dg^O86 has abnormal neuroanatomy | third instar larval stage phenotype

(Marrone et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

Dg^O86 has posterior crossvein phenotype, suppressible by Delta^9P/Dl[+]

(Christoforou et al., 2008)

Other

Statement

Reference

Dg[+]/Dg^O86, Lis-1^k11702 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg^O86, Lis-1^k13209 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg^O86, mbl^E27 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg^O86, uif¹ has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg^O86, uif^2B7 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg^O86, l(2)k09848^EMS-Mod4 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg^O86, chif^BG02820a has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg^O86, chif^EY05746 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg^O86, Fhos^EY09842 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg^O86, Itgbn^BG01037 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg^O86, Nrk^k14301 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg^O86, Pgk^KG07478 has indirect flight muscle cell | nutrition conditional phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg^O86, Fkbp14⁰⁰⁷³⁴ has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg^O86, SP2353^MB00605 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg^O86, Rack1^1.8 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg^O86, dysc^c03838 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg^O86, Rack1^EE has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg^O86, vimar⁰⁹ has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Df(3R)Exel6184/+, Dg^O86 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg^O86, dysc^c05107/dysc[+] has lamina plexus | third instar larval stage phenotype

(Marrone et al., 2011)

Cam[+]/Camⁿ³³⁹, Dg^O86 has rhabdomere | adult stage phenotype

(Marrone et al., 2011)

Cam[+]/Camⁿ³³⁹, Dg^O86 has lamina plexus | third instar larval stage phenotype

(Marrone et al., 2011)

Dg^O86, Lis-1[+]/Lis-1^k13209 has lamina plexus | third instar larval stage phenotype

(Marrone et al., 2011)

Dg^O86, chif[+]/chif^BG02820a has lamina plexus | third instar larval stage phenotype

(Marrone et al., 2011)

Df(3R)Exel6184, Dg^O86 has lamina plexus | third instar larval stage phenotype

(Marrone et al., 2011)

Dg^O86, capt^E636/capt[+] has rhabdomere | adult stage phenotype

(Marrone et al., 2011)

Dg^O86, mbl[+]/mbl^E27 has rhabdomere | adult stage phenotype

(Marrone et al., 2011)

Df(3R)Exel6184/+, Dg^O86 has rhabdomere | adult stage phenotype

(Marrone et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Dg Dys double heterozygous flies (Dg^O86/Df(3R)Exel6184) exhibit indirect flight muscle degeneration.

chif^BG02820a Dg^O86 double heterozygous flies exhibit indirect flight muscle degeneration.

chif^EY05746 Dg^O86 double heterozygous flies exhibit indirect flight muscle degeneration.

Lis-1^k11702 Dg^O86 double heterozygous flies exhibit indirect flight muscle degeneration.

Lis-1^k13209 Dg^O86 double heterozygous flies exhibit indirect flight muscle degeneration.

Nrk^k14301 Dg^O86 double heterozygous flies exhibit indirect flight muscle degeneration.

Fhos^EY09842 Dg^O86 double heterozygous flies exhibit indirect flight muscle degeneration.

Itgbn^BG01037 Dg^O86 double heterozygous flies exhibit indirect flight muscle degeneration.

CG7845^EMS-Mod4 Dg^O86 double heterozygous flies exhibit indirect flight muscle degeneration.

mbl^E27 Dg^O86 double heterozygous flies exhibit indirect flight muscle degeneration.

Rack1^EE Dg^O86 double heterozygous flies exhibit indirect flight muscle degeneration.

Rack1^1.8 Dg^O86 double heterozygous flies exhibit indirect flight muscle degeneration.

dysc^c03838 Dg^O86 double heterozygous flies exhibit indirect flight muscle degeneration.

uif^2B7 Dg^O86 double heterozygous flies exhibit indirect flight muscle degeneration.

uif¹ Dg^O86 double heterozygous flies exhibit indirect flight muscle degeneration.

Pgk^KG07478 Dg^O86 double heterozygous flies exhibit indirect flight muscle degeneration when raised on normal food, but this degeneration is not seen when flies are raised on sugar-free food. In contrast to either heterozygote alone, temperature -sensitive mobility defects are observed in the double heterozygotes.

vimar⁰⁹ Dg^O86 double heterozygous flies exhibit indirect flight muscle degeneration.

Fkbp14⁰⁰⁷³⁴ Dg^O86 double heterozygous flies exhibit indirect flight muscle degeneration.

SP2353^MB00605 Dg^O86 double heterozygous flies exhibit indirect flight muscle degeneration.

capt^E636 Dg^O86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

capt^E593 Dg^O86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

Lis-1^G10.14 Dg^O86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

nAChRα6^EY13897 Dg^O86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

nAChRα6^KG05852 Dg^O86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

Camⁿ³³⁹ Dg^O86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

Rack1^EY00128 Dg^O86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

gcm^KG01117 Dg^O86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

vimar^k16722 Dg^O86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

POSH^k15815 Dg^O86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

del^KG10262 Dg^O86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

grh^IM Dg^O86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

robo1² Dg^O86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

(Kucherenko et al., 2011)

Combination of Dg^O86 in heterozygous state with a single copy of any of the following: Df(3R)Exel6184, dysc^c05107, Camⁿ³³⁹ results in significantly increased frequency of lamina plexus defects in third instar larvae. The frequency is not significantly affected by combination with: capt^E636, vimar^EY09646, robo1², SP2353^MB00605, mbl^E27, grh^IM or Nrk^k14301 alleles and is decreased by combination with chif^BG02820a - all in heterozygous state.

Combination of Dg^O86 in heterozygous state with a single copy of any of the following :Df(3R)Exel6184, Camⁿ³³⁹, capt^E636 or mbl^E27 results in a significantly reduced rhabdomere length in the double heterozygous flies whereas combination with one copy of Nrk^k14301 has no significant effect.

The increased frequency of lamina plexus defects characteristic for Lis-1^k13209 heterozygote third instar larvae is not strongly affected by combination with a single copy of either Df(3R)Exel6184 or Dg^O86, the rhabdomere length in the adult eye of the double heterozygotes is not significantly different from the controls.

(Marrone et al., 2011)

The "detached" posterior crossvein phenotype seen in Dg^O86 homozygotes is suppressed by Dl^9P/+.

(Christoforou et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

63049

y¹ w^*; P{FRT(w^hs)}G13 sha¹ Dg^O86/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Name Synonyms

Secondary FlyBase IDs

References (21)

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