FlyBase Allele Report: Dmel\pum[UAS.fl]

General Information

Symbol

Dmel\pum^UAS.fl

Species

D. melanogaster

Name

FlyBase ID

FBal0218253

Feature type

allele

Associated gene

Dmel\pum

Associated Insertion(s)

Carried in Construct

P{UAS-pum.fl}

Key Links

Transgenic product class

cDNA

(Menon et al., 2004)

wild_type

(Menon et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Menon et al., 2004)

wild_type

(Menon et al., 2004)

Mutagen

in vitro construct

(Menon et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-pum.fl}

Cytology

Description

Full-length pum cDNA is cloned downstream of UAS regulatory sequences.

(Menon et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

pum

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | pupal stage, with Scer\GAL4^ppk.PG

(Bhogal et al., 2016)

abnormal neuroanatomy | somatic clone, with Scer\GAL4^NP0021

(Goto et al., 2011)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^ppk.PG

(Bhogal et al., 2016)

lethal, with Scer\GAL4^elav-C155

(Menon et al., 2004)

lethal, with Scer\GAL4^{Mhc.Switch.PO}

(Menon et al., 2004)

Phenotype Manifest In

Detailed Description

Statement

Reference

Animals expressing pum^Scer\UAS.fl under the control of Scer\GAL4^ppk.PG display severe dendritic arborization defects (loss of terminal branches, decreased total branch length) in class IV dendritic arborizing neurons at third instar larval stage and a significant delay in dendritic pruning of these neurons at the pupal stage.

(Bhogal et al., 2016)

Germline (Scer\GAL4^vas.PU) expression of pum^Scer\UAS.fl leads to a very mild phenotype in egg chambers, with most containing polyploid nurse cells and a small percentage showing a germ cell tumor phenotype.

(Carreira-Rosario et al., 2016)

Overexpression of pum^Scer\UAS.fl in mAL neuron clones (under the control of Scer\GAL4^fru-NP0021) demasculinizes the mAL neuron.

(Goto et al., 2011)

High-level expression of pum^Scer\UAS.fl in neurons (Scer\GAL4^elav-C155) or muscles (Scer\GAL4^{Mhc.Switch.PO}) produces lethality.

Muscle 12 neuromuscular junctions from pum^Scer\UAS.fl Scer\GAL4^elav-C155 escaper larvae, raised at 29^oC exhibit much smaller boutons than controls. The average number of type 1b boutons on muscle 12 is approximately 3-fold higher in overexpression larvae relative to controls. Similar phenotypes are observed at other neuromuscular junctions.

Expression of pum^Scer\UAS.fl to low levels in muscles (Scer\GAL4^{Mhc.Switch.PO}) at 25^oC results in no significant change in 1b bouton numbers, relative to controls.

(Menon et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl has abnormal neuroanatomy phenotype, non-suppressible by Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl

(Menon et al., 2004)

Suppressor of

Statement

Reference

Scer\GAL4^{Mhc.Switch.PO}/pum^UAS.fl is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^{Mhc.Switch.PO}, eIF4E1^UAS.cRa

(Menon et al., 2004)

NOT Suppressor of

Statement

Reference

Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl is a non-suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl

(Menon et al., 2004)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl has NMJ bouton phenotype, non-suppressible by Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl

(Menon et al., 2004)

Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl has type I bouton phenotype, non-suppressible by Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl

(Menon et al., 2004)

Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl has embryonic/larval neuromuscular junction phenotype, non-suppressible by Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl

(Menon et al., 2004)

Suppressor of

Statement

Reference

Scer\GAL4^{Mhc.Switch.PO}/pum^UAS.fl is a suppressor of embryonic/larval neuromuscular junction phenotype of Scer\GAL4^{Mhc.Switch.PO}, eIF4E1^UAS.cRa

(Menon et al., 2004)

Scer\GAL4^{Mhc.Switch.PO}/pum^UAS.fl is a suppressor of NMJ bouton phenotype of Scer\GAL4^{Mhc.Switch.PO}, eIF4E1^UAS.cRa

(Menon et al., 2004)

Scer\GAL4^{Mhc.Switch.PO}/pum^UAS.fl is a suppressor of type I bouton phenotype of Scer\GAL4^{Mhc.Switch.PO}, eIF4E1^UAS.cRa

(Menon et al., 2004)

NOT Suppressor of

Statement

Reference

Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl is a non-suppressor of NMJ bouton phenotype of Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl

(Menon et al., 2004)

Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl is a non-suppressor of type I bouton phenotype of Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl

(Menon et al., 2004)

Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl is a non-suppressor of embryonic/larval neuromuscular junction phenotype of Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl

(Menon et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Simultaneous expression of pum^Scer\UAS.fl and eIF-4E^Scer\UAS.cRa in muscles (using Scer\GAL4^{Mhc.Switch.PO}) results in the increase in 1b bouton number conferred by overexpressing eIF-4E^Scer\UAS.cRa being completely suppressed by the overexpression of pum^Scer\UAS.fl. 1b bouton numbers in larvae overexpressing both eIF-4E^Scer\UAS.cRa and pum^Scer\UAS.fl are not significantly different from those in larvae overexpressing only pum^Scer\UAS.fl.

(Menon et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^elav-C155/pum^UAS.fl rescues pum⁹/pum⁷

(Menon et al., 2004)

Scer\GAL4^{Mhc.Switch.PO}/pum^UAS.fl rescues pum⁹/pum⁷

(Menon et al., 2004)

Scer\GAL4^elav-C155, Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl rescues pum⁹/pum⁷

(Menon et al., 2004)

Fails to rescue

Scer\GAL4^elav-C155/pum^UAS.fl fails to rescue pum⁹/pum⁷

(Menon et al., 2004)

Scer\GAL4^{Mhc.Switch.PO}/pum^UAS.fl fails to rescue pum⁹/pum⁷

(Menon et al., 2004)

Scer\GAL4^elav-C155, Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl fails to rescue pum⁹/pum⁷

(Menon et al., 2004)

Comments

Expression of pum^Scer\UAS.fl at 18^oC in postmitotic neurons (Scer\GAL4^elav-C155) rescues the synaptic morphology phenotypes found in a pum⁹/pum⁷ background. Both the average size and the number of type 1b boutons on muscle 12 are restored to wild-type.

Expression of pum^Scer\UAS.fl at 18^oC in muscles (by 'leaky' expression of Scer\GAL4^{Mhc.Switch.PO} in the absence of steroid) has no effect on the phenotypes found in a pum⁹/pum⁷ background.

Expression of pum^Scer\UAS.fl in muscles (Scer\GAL4^{Mhc.Switch.PO}) in pum⁹/pum⁷ larvae reduces the number of eIF-4E aggregates by 7-fold, to 3.5 aggregates per neuromuscular junction. The level of eIF-4E is reduced by approximately 6.5-fold. In contrast, neural expression of pum^Scer\UAS.fl (Scer\GAL4^elav-C155) in pum⁹/pum⁷ larvae does not greatly decrease the number of eIF-4E aggregates (although a 20% reduction is seen). Levels of eIF-4E are 88% of those in pum⁹/pum⁷ larvae.

The increase in 1s bouton number in pum⁹/pum⁷ mutants is fully rescued through expression of pum^Scer\UAS.fl in muscles (Scer\GAL4^{Mhc.Switch.PO}), and is unaffected by expression in neurons (under the control of Scer\GAL4^elav-C155).

The mEJP amplitude and quantal content phenotypes of pum⁹/pum⁷ mutants are not rescued to near wild-type levels by neuronal (Scer\GAL4^elav-C155), muscle (Scer\GAL4^{Mhc.Switch.PO}), or dual expression of pum^Scer\UAS.fl.

Expression of pum^Scer\UAS.fl in both neurons (Scer\GAL4^elav-C155) and muscles (Scer\GAL4^{Mhc.Switch.PO}) rescues the mEJP frequency in pum⁹/pum⁷ mutants to near the level seen in pum⁹/+ heterozygotes.

(Menon et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

pum^Scer\UAS.fl

pum^UAS.fl

Name Synonyms

Secondary FlyBase IDs

References (4)

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