FlyBase Allele Report: Dmel\moi[S096713]

General Information

Symbol

Dmel\moi^S096713

Species

D. melanogaster

Name

FlyBase ID

FBal0218465

Feature type

allele

Associated gene

Dmel\moi

Associated Insertion(s)

P{lacW}moi^S096713

Carried in Construct

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Komonyi et al., 2009, Raffa et al., 2009)

Mutagen

Progenitor genotype

Associated Insertion(s)

P{lacW}moi^S096713

Cytology

Description

P{lacW} insertion upstream of the upstream open reading frame (moi) of the dicistronic locus that encodes both moi and tatl.

(Raffa et al., 2009)

Insertion 35bp downstream of the tatl and moi common transcriptional start.

(Komonyi et al., 2005)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior (with Df(3R)d189), with moi^um.t4.6

(Komonyi et al., 2009)

lethal (with moi¹)

(Raffa et al., 2009)

lethal | recessive

(Raffa et al., 2009)

lethal - all die during pupal stage (with Df(3R)d189)

(Komonyi et al., 2009)

short lived (with Df(3R)d189), with moi^um.t4.6

(Komonyi et al., 2009)

viable (with Df(3R)d189), with moi^um.t4.6

(Komonyi et al., 2009)

Phenotype Manifest In

adult thoracic sensillum (with Df(3R)d189), with moi^um.t4.6

(Komonyi et al., 2009)

chromosome, telomeric region (with Df(3R)d189), with moi^um.t4.6

(Komonyi et al., 2009)

chromosome, telomeric region (with moi¹)

(Raffa et al., 2009)

chromosome, telomeric region (with moi^CB02140)

(Raffa et al., 2009)

eye (with Df(3R)d189), with moi^um.t4.6

(Komonyi et al., 2009)

wing margin (with Df(3R)d189), with moi^um.t4.6

(Komonyi et al., 2009)

wing vein (with Df(3R)d189), with moi^um.t4.6

(Komonyi et al., 2009)

Detailed Description

Statement

Reference

P{lacW}moi^S096713/Df(3R)d189 animals carrying P{CaSpeR-DTLu^m} (i.e. moi mutants) eclose and survive for a short time, exhibiting signs of locomotor defects, rough eyes, missing wing veins, scalloped wings and missing thoracic bristles.

(Komonyi et al., 2009)

Mitotic cells from the brains of moi¹/moi^S096713 and and moi^S096713/moi^CB02140 larvae show frequent telomere fusions.

(Raffa et al., 2009)

Flies homozygous for the P{lacW}moi^S096713 insertion die as third instar larvae.

Homozygotes die as third instar larvae.

(Deak et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Df(3R)d189/moi^S096713, moi^um.t4.6 has short lived phenotype, enhanceable by tefu^atm-6

(Komonyi et al., 2009)

Enhancer of

Statement

Reference

moi^um.t4.6, Df(3R)d189, moi^S096713 is an enhancer of lethal - all die before end of P-stage phenotype of tefu^atm-6

(Komonyi et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Df(3R)d189/moi^S096713, moi^um.t4.6 has chromosome phenotype, enhanceable by tefu^atm-6

(Komonyi et al., 2009)

Suppressed by

Statement

Reference

Df(3R)d189/moi^S096713, moi^um.t4.6 has chromosome phenotype, suppressible by mei-41^RT1

(Komonyi et al., 2009)

Enhancer of

Statement

Reference

moi^um.t4.6, Df(3R)d189, moi^S096713 is an enhancer of chromosome phenotype of tefu^atm-6

(Komonyi et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

tefu^atm-6 flies that also carry P{lacW}moi^S096713/Df(3R)d189, P{CaSpeR-DTLu^m} (i.e. moi mutants) die at an earlier phase than either single mutant condition.

tefu^atm-6 neuroblasts that also carry P{lacW}moi^S096713/Df(3R)d189, P{CaSpeR-DTLu^m} (i.e. moi mutants) show an increased number of anaphase chromosome bridges than either single mutant condition.

P{lacW}moi^S096713/Df(3R)d189, P{CaSpeR-DTLu^m} (i.e. moi mutant) neuroblasts show fewer anaphase chromosome bridges when mei-41^RT1 is present.

(Komonyi et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

moi¹

(Raffa et al., 2009)

Rescued by

Df(3R)d189/moi^S096713 is rescued by moi^+t4.6

(Komonyi et al., 2009)

moi¹/moi^S096713 is rescued by moi^R1.Tub

(Raffa et al., 2009)

moi¹/moi^S096713 is rescued by moi^R2.Tub

(Raffa et al., 2009)

moi¹/moi^S096713 is rescued by moi^R3.Tub

(Raffa et al., 2009)

Not rescued by

Df(3R)d189/moi^S096713 is not rescued by moi^dm.t4.6

(Komonyi et al., 2009)

Comments

P{CaSpeR-DTL} (expressing wild type moi and Tgs1) rescues the lethality of P{lacW}moi^S096713/Df(3R)d189.

The presence of P{CaSpeR-DTLd^m} (expressing wild type moi) does not change the lethality phase of P{lacW}moi^S096713/Df(3R)d189.

(Komonyi et al., 2009)

The P3-P4 early pupal lethality seen in P{lacW}moi^S096713/Df(3R)d189 animals is completely rescued by P{CaSpeR-DTL} (which carries moi^+t4.6 and tatl^+t4.6).

The P3-P4 early pupal lethality seen in P{lacW}moi^S096713/Df(3R)d189 animals is not rescued by P{CaSpeR-DTLd^m} (which carries tatl^dm.t4.6 and moi^dm.t4.6).

The P3-P4 early pupal lethality seen in P{lacW}moi^S096713/Df(3R)d189 animals is partially rescued by P{CaSpeR-DTLu^m} (which carries tatl^um.t4.6 and moi^um.t4.6); animals die as pharate adults or immediately after hatching.

The P3-P4 early pupal lethality seen in P{lacW}moi^S096713/Df(3R)d189 animals is completely rescued by P{CaSpeR-DTLd^m} and P{CaSpeR-DTLu^m} together in trans.

(Komonyi et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Associated with: tatl^S096713.

(Raffa et al., 2009)

The P{lacW}moi^S096713 insertion maps upstream of a dicistronic locus that encodes moi (upstream ORF) and tatl (downstream ORF). Rescue analysis indicates that the telomere fusion phenotype seen in P{lacW}moi^S096713 mutants is due to an effect on the moi ORF and not on tatl. In contrast, the lethality caused by the insertion is not rescued by a transgene containing either ORF alone, but is rescued by a transgene containing functional copies of both ORFs, indicating that the lethality is due to an effect on both ORFs.

(Raffa et al., 2009)

Comments

Allelic series (with respect to severity of telomere fusion phenotype): moi¹ > moi^CB02140 = moi^M12 > moi^S096713.

(Raffa et al., 2009)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

l(3)S096713

(Raffa et al., 2009)

moi^S096713

moi^S09

(Raffa et al., 2009)

tlu^S096713

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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