An approximately 1kb fragment consisting of the complete intergenic region (249bp) between Phm and its neighbouring upstream gene and Phm sequences through to and including the complete first intron are fused upstream of sequence encoding a GAL4 driver. The Phm AUG codon that is present in the first Phm exon included in this fragment has been mutated (to prevent initiation from this site).
ScerGAL4Phm.WT drives expression in neurons in the adult and larval brain and ventral nerve cord. Most of these cells co-express PICK1 protein.