FlyBase Allele Report: Dmel\Lrrk[unspecified]

General Information

Symbol

Dmel\Lrrk^unspecified

Species

D. melanogaster

Name

FlyBase ID

FBal0220789

Feature type

allele

Associated gene

Dmel\Lrrk

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

FlyBase curator comment: this entry is used to capture phenotypic information when the particular allele (or allele combination) used by the author could not be determined but the context of the experiment suggests that the phenotype being described is some kind of loss of function.

(FlyBase Curators, 2025-)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell death

(Dodson et al., 2014)

abnormal neuroanatomy | larval stage

(Dodson et al., 2014)

chemical resistant

(Imai et al., 2008)

female semi-fertile

(Imai et al., 2008)

viable

(Imai et al., 2008)

Phenotype Manifest In

adult abdomen | female

(Imai et al., 2008)

early endosome

(Dodson et al., 2014)

embryonic/larval neuromuscular junction

(Dodson et al., 2014)

follicle cell

(Dodson et al., 2014)

late endosome

(Dodson et al., 2014)

lysosome

(Dodson et al., 2014)

NMJ bouton | increased number

(Dodson et al., 2014)

Detailed Description

Statement

Reference

Lrrk mutants undergo premature follicle cell death during stages 11-13 of egg chamber development. Lrrk mutant follicle cells show markedly enlarged early endosomal, late endosomal and lysosomal structures.

Lrrk loss of function does not exhibit lysosome clustering.

Lrrk mutants show synaptic overgrowth at neuromuscular junctions, with a significant increase of supernumerary satellite boutons surrounding the larger, normal boutons.

(Dodson et al., 2014)

Mutant females often have a malformed abdomen, especially if nutritional status is compromised at the larval stage.

The number of dopaminergic neurons in the protocerebral posterior medial 1 and 2 clusters and in the protocerebral posterior lateral 1 cluster and the number of neurons in the protocerebral posterior medial 3 cluster is normal in 60 day old Lrrk^unspecified adults.

Homozygotes and hemizygotes show significantly higher resistance to paraquat and to hydrogen peroxide compared to control flies.

(Imai et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Lrrk^unspecified has abnormal cell death phenotype, enhanceable by Atg7^unspecified

(Dodson et al., 2014)

Suppressed by

Statement

Reference

Lrrk^unspecified has abnormal cell death phenotype, suppressible | partially by Scer\GAL4^CY2/Diap1^UASp.cUa

(Dodson et al., 2014)

Lrrk^unspecified has abnormal cell death phenotype, suppressible | partially by Scer\GAL4^CY2/Rab9^{Q71L.UASp.YFP}

(Dodson et al., 2014)

Lrrk^unspecified has abnormal neuroanatomy | larval stage phenotype, suppressible | partially by Rab9^{Q71L.UASp.YFP}/Scer\GAL4^RapGAP1-OK6

(Dodson et al., 2014)

Lrrk^unspecified has abnormal cell death phenotype, suppressible | partially by Scer\GAL4^CY2/Hsap\LRRK2^UAS.Tag:HA

(Dodson et al., 2014)

Suppressor of

Statement

Reference

Lrrk^unspecified/Lrrk^unspecified is a suppressor of abnormal neuroanatomy | adult stage phenotype of Scer\GAL4^ple.PF, eIF4E1^UAS.cRa

(Imai et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

Lrrk^unspecified has follicle cell phenotype, enhanceable by Atg7^unspecified

(Dodson et al., 2014)

Suppressed by

Statement

Reference

Lrrk^unspecified has lysosome phenotype, suppressible | partially by Scer\GAL4^CY2/Rab9^{Q71L.UASp.YFP}

(Dodson et al., 2014)

Lrrk^unspecified has follicle cell phenotype, suppressible | partially by Scer\GAL4^CY2/Hsap\LRRK2^UAS.Tag:HA

(Dodson et al., 2014)

Lrrk^unspecified has follicle cell phenotype, suppressible by Scer\GAL4^CY2/Diap1^UASp.cUa

(Dodson et al., 2014)

Lrrk^unspecified has follicle cell phenotype, suppressible by Scer\GAL4^CY2/Rab9^{Q71L.UASp.YFP}

(Dodson et al., 2014)

Lrrk^unspecified has embryonic/larval neuromuscular junction phenotype, suppressible | partially by Rab9^{Q71L.UASp.YFP}/Scer\GAL4^RapGAP1-OK6

(Dodson et al., 2014)

Lrrk^unspecified has NMJ bouton | increased number phenotype, suppressible | partially by Rab9^{Q71L.UASp.YFP}/Scer\GAL4^RapGAP1-OK6

(Dodson et al., 2014)

NOT suppressed by

Statement

Reference

Lrrk^unspecified has lysosome phenotype, non-suppressible by Scer\GAL4^CY2/Diap1^UASp.cUa

(Dodson et al., 2014)

Lrrk^unspecified has lysosome phenotype, non-suppressible by Atg7^unspecified

(Dodson et al., 2014)

Lrrk^unspecified has lysosome phenotype, non-suppressible by Scer\GAL4^CY2/Rab7^{T22N.UASp.YFP}

(Dodson et al., 2014)

Lrrk^unspecified has lysosome phenotype, non-suppressible by Scer\GAL4^CY2/Rab7^{Q67L.UASp.YFP}

(Dodson et al., 2014)

Suppressor of

Statement

Reference

Lrrk^unspecified/Lrrk^unspecified is a suppressor of dopaminergic neuron phenotype of Scer\GAL4^ple.PF, eIF4E1^UAS.cRa

(Imai et al., 2008)

Other

Statement

Reference

Atg7^unspecified, Lrrk^unspecified has eye phenotype

(Dodson et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Diap1^{Scer\UAS.P\T.cUa} under the control of Scer\GAL4^CY2 partially rescues the premature follicle cell death phenotype of Lrrk mutants, but does not rescue the lysosome enlargement phenotype of Lrrk mutants.

Removing Atg7 function in Lrrk mutants results in a significant increase in follicle cell death, but no change in the lysosome enlargement phenotype of Lrrk mutants.

Double mutants of Atg7 and Lrrk display a mild rough-eye phenotype.

Expression of Rab7^{T22N.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^CY2 does not change the average lysosome size in Lrrk mutants.

Expression of Rab7^{Q67L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^CY2 does not change the average lysosome size in Lrrk mutants.

Expression of Rab9^{Q71L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^CY2 partially suppresses the lysosome enlargement and premature follicle cell death phenotypes observed in Lrrk mutants.

Expression of Rab9^{Q71L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^RapGAP1-OK6 partially rescues the neuromuscular junction synapse overgrowth phenotype of Lrrk mutants.

(Dodson et al., 2014)

The decrease in the number of dopaminergic neurons in the protocerebral posterior medial 1 and 2 clusters and in the protocerebral posterior lateral 1 cluster that is seen in 60 day old adults expressing eIF-4E^Scer\UAS.cRa under the control of Scer\GAL4^ple.PF is suppressed if the flies are also homozygous for Lrrk^unspecified.

(Imai et al., 2008)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\LRRK2^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^CY2 partially rescues the premature follicle cell death phenotype and enlarged-lysosome phenotype of Lrrk mutants.

(Dodson et al., 2014)

Complementation and Rescue Data

Partially rescued by

Lrrk^unspecified is partially rescued by Scer\GAL4^CY2/Lrrk^UAS.cDa

(Dodson et al., 2014)

Lrrk^unspecified is partially rescued by Scer\GAL4^RapGAP1-OK6/Lrrk^UAS.cDa

(Dodson et al., 2014)

Lrrk^unspecified is partially rescued by Scer\GAL4^CY2/Lrrk^G1914S.UAS

(Dodson et al., 2014)

Lrrk^unspecified is partially rescued by Lrrk^Tag:MYC

(Dodson et al., 2014)

Comments

Expression of Lrrk^Scer\UAS.cDa under the control of Scer\GAL4^CY2 partially rescues the premature follicle cell death phenotype and enlarged-lysosome phenotype of Lrrk mutants.

Expression of Lrrk^T:Hsap\MYC partially rescues the premature follicle cell death phenotype of Lrrk mutants.

Expression of Lrrk^{G1914S.Scer\UAS} under the control of Scer\GAL4^CY2 partially rescues the enlarged-lysosome phenotype of Lrrk mutants.

Expression of Lrrk^Scer\UAS.cDa under the control of Scer\GAL4^RapGAP1-OK6 partially rescues the neuromuscular junction synapse overgrowth phenotype of Lrrk mutants.

(Dodson et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Lrrk^unspecified

dLRRK^-

(Imai et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (4)

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