FlyBase Allele Report: Dmel\Gem3[e03688]

General Information

Symbol

Dmel\Gem3^e03688

Species

D. melanogaster

Name

FlyBase ID

FBal0220796

Feature type

allele

Associated gene

Dmel\Gem3

Associated Insertion(s)

PBac{?RB}Gem3^e03688

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Shpargel et al., 2009)

Mutagen

Progenitor genotype

Associated Insertion(s)

PBac{?RB}Gem3^e03688

Cytology

Description

Insertion 92bp upstream of the transcription start site. Part of the 5' and 3' ends of the original PBac{RB} element have been lost during the insertion.

(Cauchi et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:10,463,175..10,463,175 [+]

(Garcia et al., 2024, Shpargel et al., 2009, Cauchi et al., 2008)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

anterior pupal spiracle (with Gem3^rL562)

(Cauchi et al., 2008)

embryonic/larval neuromuscular junction (with Gem3^rL562)

(Cauchi et al., 2008)

puparium cuticle (with Gem3^rL562)

(Cauchi et al., 2008)

Detailed Description

Statement

Reference

30% of homozygous larvae survive to day 8 after egg laying (a period when the control larvae have already pupated) but fail to pupate, although a few animals form pseudopupal cases. A small fraction of homozygous larvae survive to day 25 after egg laying.

Homozygous larvae are thinner and shorter than control larvae at 4 days after egg laying.

Homozygous second-third instar larvae show impaired motor function, showing a smaller distance travelled in the 20 seconds after movement was stimulated with a needle than control larvae.

(Shpargel et al., 2009)

Gem3^rL562/Gem3^e03688 animals survive to the prepupal stage. The animals undergo a prolonged wandering third instar larval stage.

Gem3^rL562/Gem3^e03688 animals show normal body wall contraction rates throughout the first and second larval instars, but at the third instar larval stage the body wall contraction rate is significantly decreased in the mutant animals compared to controls. The mutant third instar larvae show no apparent disruption in the gross structure of the body wall musculature or in sarcomere organisation.

The puparium made by Gem3^rL562/Gem3^e03688 animals fails to evert spiracles and has a large axial ratio (length/width).

Gem3^rL562/Gem3^e03688 larvae show a synaptic overgrowth phenotype at the neuromuscular junction (NMJ) and have a significantly increased synaptic area when it is normalised to muscle size.

(Cauchi et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Gem3^rL562/Gem3^e03688 has lethal | larval stage phenotype, non-suppressible by Smn^UASp.Venus/Scer\GAL4^da.G32

(Shpargel et al., 2009)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Gem3^rL562

(Shpargel et al., 2009)

Gem3^rL562

(Cauchi et al., 2008)

Rescued by

Gem3^e03688 is rescued by Gem3^UAS.Tag:FLAG/Scer\GAL4^da.G32

(Shpargel et al., 2009)

Gem3^rL562/Gem3^e03688 is rescued by Scer\GAL4^1032.hx/Gem3^UAS.ECFP

(Cauchi et al., 2008)

Gem3^rL562/Gem3^e03688 is rescued by Scer\GAL4^1032.hx/Gem3^UAS.cCa

(Cauchi et al., 2008)

Partially rescued by

Gem3^rL562/Gem3^e03688 is partially rescued by Gem3^UAS.Tag:FLAG/Scer\GAL4^da.G32

(Shpargel et al., 2009)

Not rescued by

Gem3^rL562/Gem3^e03688 is not rescued by Scer\GAL4^G7/Gem3^UAS.ECFP

(Cauchi et al., 2008)

Gem3^rL562/Gem3^e03688 is not rescued by Scer\GAL4^elav-C155/Gem3^UAS.ECFP

(Cauchi et al., 2008)

Gem3^e03688 is not rescued by Scer\GAL4^1032.hx/Gem3^UAS.cCa

(Cauchi et al., 2008)

Gem3^e03688 is not rescued by Scer\GAL4^1032.hx/Gem3^UAS.ECFP

(Cauchi et al., 2008)

Comments

Expression of Gem3^{Scer\UAS.T:Avic\GFP-CFP} under the control of Scer\GAL4^1032.hx rescues the mobility, spiracle eversion and abnormal axial ratio phenotypes seen in Gem3[rL562]/Gem3[e03688] mutants.

(Cauchi et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

The lethality of the homozygous Gem3^e03688 chromosome cannot be rescued by expression of Gem3^{Scer\UAS.T:Avic\GFP-CFP} under the control of Scer\GAL4^1032.hx, suggesting that there may be a secondary lethal mutation on the Gem3^e03688 chromosome (since the Gem3^{Scer\UAS.T:Avic\GFP-CFP}, Scer\GAL4^1032.hx combination can rescue the lethality of Gem3^e03688/Gem3^rL562 and Gem3^rL562/Gem3^rL562 animals).

(Cauchi et al., 2008)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Gem3^A

(Shpargel et al., 2009)

Gem3^e03688

gemin3^W

(Cauchi et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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