FlyBase Allele Report: Dmel\Gem3[rL562]

(Garcia et al., 2024, Shpargel et al., 2009, Cauchi et al., 2008, Beaton, 2000.1.31, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | larval stage

(Shpargel et al., 2009)

abnormal locomotor behavior | larval stage

(Shpargel et al., 2009)

abnormal locomotor behavior | third instar larval stage (with Gem3^e03688)

(Cauchi et al., 2008)

abnormal neuroanatomy | larval stage (with Gem3^e03688)

(Cauchi et al., 2008)

decreased body size | larval stage

(Shpargel et al., 2009)

lethal (with Df(3L)ED4457)

(Cauchi et al., 2008)

lethal (with Gem3^e03688)

(Cauchi et al., 2008)

lethal | recessive

(Cauchi et al., 2008, Beaton, 2000.1.31, Spradling et al., 1999, BDGP Project Members, 1994-1999)

lethal - all die before end of larval stage (with Gem3^e03688)

(Shpargel et al., 2009)

lethal - all die before end of larval stage | recessive

(Shpargel et al., 2009)

some die during larval stage (with Gem3^e03688)

(Shpargel et al., 2009)

some die during larval stage | recessive

(Shpargel et al., 2009)

Phenotype Manifest In

anterior pupal spiracle (with Gem3^e03688)

(Cauchi et al., 2008)

embryonic/larval neuromuscular junction (with Gem3^e03688)

(Cauchi et al., 2008)

puparium cuticle (with Gem3^e03688)

(Cauchi et al., 2008)

Detailed Description

Statement

Reference

15% of homozygous larvae survive to day 8 after egg laying (a period when the control larvae have already pupated) but fail to pupate. A small fraction of homozygous larvae survive to day 25 after egg laying.

Homozygous larvae are thinner and shorter than control larvae at 4 days after egg laying. The mouth hooks of these animals are more similar to those of wild-type second instar larvae, whereas control animals at this time have entered the third larval instar stage.

Homozygous second-third instar larvae show impaired motor function, showing a smaller distance travelled in the 20 seconds after movement was stimulated with a needle than control larvae.

(Shpargel et al., 2009)

Homozygotes survive to the third instar larval stage, while Gem3^rL562/Gem3^e03688 animals survive to the prepupal stage. Both genotypes undergo a prolonged wandering third instar larval stage.

Gem3^rL562/Gem3^e03688 animals show normal body wall contraction rates throughout the first and second larval instars, but at the third instar larval stage the body wall contraction rate is significantly decreased in the mutant animals compared to controls. The mutant third instar larvae show no apparent disruption in the gross structure of the body wall musculature or in sarcomere organisation.

The puparium made by Gem3^rL562/Gem3^e03688 animals fails to evert spiracles and has a large axial ratio (length/width).

Gem3^rL562/Gem3^e03688 larvae show a synaptic overgrowth phenotype at the neuromuscular junction (NMJ) and have a significantly increased synaptic area when it is normalised to muscle size.

(Cauchi et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Gem3^rL562/Gem3^e03688 has lethal | larval stage phenotype, non-suppressible by Smn^UASp.Venus/Scer\GAL4^da.G32

(Shpargel et al., 2009)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Gem3^e03688

(Shpargel et al., 2009)

Gem3^e03688

(Cauchi et al., 2008)

Rescued by

Gem3^rL562/Gem3^e03688 is rescued by Scer\GAL4^1032.hx/Gem3^UAS.ECFP

(Cauchi et al., 2008)

Gem3^rL562/Gem3^e03688 is rescued by Scer\GAL4^1032.hx/Gem3^UAS.cCa

(Cauchi et al., 2008)

Gem3^rL562 is rescued by Scer\GAL4^1032.hx/Gem3^UAS.ECFP

(Cauchi et al., 2008)

Gem3^rL562 is rescued by Scer\GAL4^1032.hx/Gem3^UAS.cCa

(Cauchi et al., 2008)

Partially rescued by

Gem3^rL562/Gem3^e03688 is partially rescued by Gem3^UAS.Tag:FLAG/Scer\GAL4^da.G32

(Shpargel et al., 2009)

Gem3^rL562 is partially rescued by Gem3^UAS.Tag:FLAG/Scer\GAL4^da.G32

(Shpargel et al., 2009)

Not rescued by

Gem3^rL562/Gem3^e03688 is not rescued by Scer\GAL4^G7/Gem3^UAS.ECFP

(Cauchi et al., 2008)

Gem3^rL562/Gem3^e03688 is not rescued by Scer\GAL4^elav-C155/Gem3^UAS.ECFP

(Cauchi et al., 2008)

Gem3^rL562 is not rescued by Scer\GAL4^G7/Gem3^UAS.ECFP

(Cauchi et al., 2008)

Gem3^rL562 is not rescued by Scer\GAL4^elav-C155/Gem3^UAS.ECFP

(Cauchi et al., 2008)

Comments

Expression of Gem3^{Scer\UAS.T:Avic\GFP-CFP} under the control of Scer\GAL4^1032.hx rescues the mobility, spiracle eversion and abnormal axial ratio phenotypes seen in Gem3[rL562]/Gem3[e03688] mutants.

(Cauchi et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

12079

P{PZ}Gem3^rL562 ry⁵⁰⁶/TM3, Sb¹

Notes on Origin

Discoverer

G. Rubin.

Comments

Reversion analysis proved that the P{PZ} is responsible for the lethal phenotype.

(Beaton, 2000.1.31)

Precise excision of the insertion reverts the lethal phenotype.

(Cauchi et al., 2008)

Complements: simj⁰¹⁸¹⁴. Complements: cpo⁰¹⁸²⁴. Complements: l(3)L0539^L0539. Complements: simj^j4A5.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Dhh1^rL562

Gem3^B

(Shpargel et al., 2009)

Gem3^rL562

gemin3^R

(Cauchi et al., 2008)

l(3)rL562

(Spradling et al., 1999)

l(3)rL562^rL562

(Meister and Braun, 1995.10, BDGP Project Members, 1994-1999)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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