FlyBase Allele Report: Dmel\vib[j5A6]

(Reddy et al., 2022, Gatt and Glover, 2006, Giansanti et al., 2006, Giansanti et al., 2006, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity | larval stage (with Df(3R)BSC850)

(Koe et al., 2018)

abnormal cell polarity | larval stage (with vib¹³³)

(Koe et al., 2018)

abnormal cell polarity | larval stage (with vib¹¹⁰⁵)

(Koe et al., 2018)

abnormal cytokinesis

(Giansanti et al., 2006)

abnormal cytokinesis | larval stage (with vib¹³³)

(Koe et al., 2018)

lethal

(Giansanti et al., 2006)

lethal | embryonic stage | maternal effect

(Perrimon et al., 1996)

lethal | recessive

(Spradling et al., 1999, Perrimon et al., 1996)

lethal - all die before end of larval stage (with Df(3R)BSC850)

(Koe et al., 2018)

lethal - all die before end of P-stage

(Gatt and Glover, 2006)

lethal - all die before end of P-stage (with Df(3R)Delta-BX12)

(Gatt and Glover, 2006)

some die during pharate adult stage

(Gatt and Glover, 2006)

some die during pharate adult stage (with Df(3R)Delta-BX12)

(Gatt and Glover, 2006)

sterile (with Df(3R)Delta-BX12)

(Gatt and Glover, 2006)

Phenotype Manifest In

embryonic/first instar larval cuticle | maternal effect

(Perrimon et al., 1996)

head | maternal effect

(Perrimon et al., 1996)

larval brain

(Giansanti et al., 2006)

larval brain | larval stage (with vib¹³³)

(Koe et al., 2018)

neuroblast | larval stage (with Df(3R)BSC850)

(Koe et al., 2018)

neuroblast | larval stage (with vib¹³³)

(Koe et al., 2018)

neuroblast | larval stage (with vib¹¹⁰⁵)

(Koe et al., 2018)

ventral denticle belt | embryonic stage | maternal effect

(Perrimon et al., 1996)

Detailed Description

Statement

Reference

vib¹³³/vib^j5A6 or vib¹¹⁰⁵/vib^j5A6 transheterozygous larval brain neuroblasts frequently display defective asymmetric cell divisions, as compared to controls: aPKC, Par6, and Gαi, but not Baz, Insc, Pins and Cdc42, fail to localize to a strong apical cortex crescent during metaphase; Mira, Pros, Brat, Numb and Pon fail to localize to a strong basal cortex crescent during metaphase; and the mitotic spindle axis is misoriented with respect to the neuroblast polarity, although centrosomes and spindle architecture are apparently unaffected. Similarly, vib^j5A6/Df(3R)BSC850 transheterozygous larval brain neuroblasts also frequently fail to localize aPKC and Mira to opposite apical and basal cortex crescents, respectively, during metaphase. vib¹³³/vib^j5A6 or vib¹¹⁰⁵/vib^j5A6 transheterozygous larval brain neuroblasts display highly prevalent cytokinesis defects, as compared to controls.

(Koe et al., 2018)

Larval brains of vib^j5A6 mutants exhibit frequent polyploid cells but have normal frequences of anaphases, consistent with a defect in neuroblast cytokinesis.

(Giansanti et al., 2006)

Germline clones produce eggs with patterning defects: variable deletion of denticle belts and head defects. Lethality occurs during the larval and pupal stages.

(Perrimon et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

vib¹³³/vib^j5A6 has abnormal cell polarity | larval stage phenotype, non-suppressible by Rok^Ubi-p63E.GFP

(Koe et al., 2018)

vib¹³³/vib^j5A6 has abnormal cell polarity | larval stage phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/Rok^CAT.UAS

(Koe et al., 2018)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

vib¹³³/vib^j5A6 has larval brain | larval stage phenotype, non-suppressible by Rok^Ubi-p63E.GFP

(Koe et al., 2018)

vib¹³³/vib^j5A6 has neuroblast | larval stage phenotype, non-suppressible by Rok^Ubi-p63E.GFP

(Koe et al., 2018)

vib¹³³/vib^j5A6 has larval brain | larval stage phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/Rok^CAT.UAS

(Koe et al., 2018)

vib¹³³/vib^j5A6 has neuroblast | larval stage phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/Rok^CAT.UAS

(Koe et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

vib^j7A3

(BDGP Project Members, 1994-1999)

vib^j7C8

(BDGP Project Members, 1994-1999)

vib^j4A3

(BDGP Project Members, 1994-1999)

Partially rescued by

vib¹³³/vib^j5A6 is partially rescued by vib^{T63E.UAS.Venus}/Scer\GAL4^insc-Mz1407

(Koe et al., 2018)

Not rescued by

vib¹³³/vib^j5A6 is not rescued by Scer\GAL4^insc-Mz1407/vib^{T63A.UAS.Venus}

(Koe et al., 2018)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

12144

y¹ w^*; P{lacW}vib^j5A6/TM6B, Tb⁺

Kyoto

111418

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}82B P{lacW}vib^j5A6/TM6B, P{Car20y}TPN1, Tb¹

Notes on Origin

Discoverer

L. and Y. Jan.

Comments

Complements: sqz⁰²¹⁰². Complements: l(3)03346⁰³³⁴⁶. Complements: l(3)03675⁰³⁶⁷⁵. Complements: l(3)06404⁰⁶⁴⁰⁴. Complements: nos⁰⁷¹¹⁷. Complements: nos^j3B6. Complements: nos^j4B6. Complements: l(3)s1922^s1922.

(BDGP Project Members, 1994-1999)

Based on the lethal stage of hemizygous alleles (over Df(3R)Dl-BX12), the following vib alleles can be ranked from strongest to weakest as follows: vib^j7A3 > vib^S110416 > vib^EP513 = vib^j5A6 > vib^S045002c > vib^EP651.

(Gatt and Glover, 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

gio^j5A6

(Giansanti et al., 2006, Giansanti et al., 2006)

l(3)j5A6

(Spradling et al., 1999)

l(3)j5A6^j5A6

vib^j5A6

(Koe et al., 2018, Gatt and Glover, 2006)

Name Synonyms

Secondary FlyBase IDs

FBal0219586
FBal0010964

References (10)

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