FlyBase Insertion Report: Dmel\P{lacW}vib[j5A6]

General Information

Symbol

Dmel\P{lacW}vib^j5A6

Species

D. melanogaster

Name

FlyBase ID

FBti0004968

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{lacW}

LINE ID

l(3)j5A6

Affected gene(s)

Ecol\lacZ, vib

Viability / fertility

Associated allele(s)

Ecol\lacZ^j5A6, vib^j5A6

Stock availability

2 publicly available

Genomic Location

Chromosomal Location

3R ( 91F12 )

Sequence Location

3R:19,226,365..19,226,365 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{lacW}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w^+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3R:19,226,365..19,226,365 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

91F12 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ034088

(FlyBase, 1992-)

Inserted Element

Construct

P{lacW}

(Giansanti et al., 2006)

Size (Kb)

10.691

Component Allele(s)

w^+mC

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(BDGP Project Members, 1994-1999)

vib

(Reddy et al., 2022, Gatt and Glover, 2006, Giansanti et al., 2006, Giansanti et al., 2006, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^j5A6

Phenotypic Class Phenotype Manifest in Detailed Description

vib^j5A6

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

gastric caecum

(Meister and Braun, 1995.10)

embryonic/larval salivary gland

(Meister and Braun, 1995.10)

embryonic/larval fat body

(Meister and Braun, 1995.10)

embryonic/larval midgut

(Meister and Braun, 1995.10)

embryonic/larval hindgut

(Meister and Braun, 1995.10)

embryonic/larval proventriculus

(Meister and Braun, 1995.10)

Additional Information

Statement

Reference

In wandering third instar larva, expressed in various tissues with highest levels observed in the proventriculus.

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

vib

(Meister and Braun, 1995.10)

Reporter construct used in assay

Data on Genetic Line

Line ID

l(3)j5A6

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (2)

Bloomington

12144

y¹ w^*; P{lacW}vib^j5A6/TM6B, Tb⁺

Kyoto

111418

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}82B P{lacW}vib^j5A6/TM6B, P{Car20y}TPN1, Tb¹

External Crossreferences and Linkouts ( 0 )

Comments

Location 3R:15052087-15052088 confirmed by FlyBase alignment of dbGSS accession AQ034088 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.

(FlyBase, 2005)

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

P2144

(Meister and Braun, 1995.10)

P{lacW}l(3)j5A6^j5A6

(FlyBase, 2005, BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{lacW}vib^j5A6

(Reddy et al., 2022)

P{w^+mC=lacW}l(3)j5A6^j5A6

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0001185

References (14)

Report Sections

Open Close