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General Information
Symbol
Dmel\sqhRLC.GFP(S65T)
Species
D. melanogaster
Name
FlyBase ID
FBal0221190
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Sqh-GFP, Sqh::GFP, RLC-GFP, P{sqh-GFP}, sqhGFP, MRLC-GFP, sqh-sqh:GFP, MyoII-GFP, Sqh::Sqh-GFP, Myosin-GFP
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

The sqh coding region, which is tagged at the C-terminal end with a GFP(S65T) tag, is expressed under the control of the natural sqh promoter.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

sqhRLC.GFP(S65T) protein is associated with the basolateral cortex of neuroepithelial cells in the larval optic anlage, where it interacts with GFP-tagged asp protein.

 
Marker for
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

In ex vivo third instar larval eye-antennal discs expressing sqhRLC.T:Avic\GFP-S65T, chromophore-assisted laser inactivation (CALI) of sqhRLC.T:Avic\GFP-S65T leads to a significant reduction in the extent of epithelial folding at the A1 fold, and disruption of the field boundary of the A1 fold, allowing cells to cross the boundary, in contrast to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhancer of
Additional Comments
Genetic Interactions
Statement
Reference

sqhRLC.T:Avic\GFP-S65T/sqhRLC.T:Avic\GFP-S65T enhances the shorter egg length phenotype seen in Pkn1T/Pkn1T or Pkn2T/Pkn2T germline clones.

Xenogenetic Interactions
Statement
Reference

sqhAX3 embryos which carry sqhRLC.T:Avic\GFP-S65T and which also express slmbScer\UAS.T:Zzzz\vhhGFP4 under the control of Scer\GAL4332.3 in the postmitotic amnioserosa cells show a dorsal open phenotype.

Complementation and Rescue Data
Comments

Expression of sqhRLC.T:Avic\GFP-S65T rescues the loss of rotation in the proventriculus and anterior midgut seen in sqhAX3/Y.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Carried in plasmid "RLC-GFP" and transfected into S2 cells.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
sqhRLC.GFP(S65T)
sqhRLC.T:Avic\GFP-S65T
Name Synonyms
Secondary FlyBase IDs
  • FBal0119137
  • FBal0177671
References (111)