FlyBase Allele Report: Dmel\Pkn[1T]

General Information

Symbol

Dmel\Pkn^1T

Species

D. melanogaster

Name

FlyBase ID

FBal0318521

Feature type

allele

Associated gene

Dmel\Pkn

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Ferreira et al., 2014)

Mutagen

Progenitor genotype

Cytology

Description

Splice site mutation (G to A) within the kinase domain. This is predicted to cause intron retention and mis-translation of the intronic fragment after amino acid residue 1277, resulting in a truncation of the kinase domain.

(Ferreira et al., 2014)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:9,254,650..9,254,650 [-]

Nucleotide change:

G9254650A

Reported nucleotide change:

G?A

Comment:

Reported as a G to A mutation in a splice site after amino acid residue 1277. The position of mutation on reference sequence was inferred by a FlyBase curator. The isoforms relative to which the mutation was mapped was not specified.

(Ferreira et al., 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cytoskeleton | germline clone

(Ferreira et al., 2014)

egg | germline clone

(Ferreira et al., 2014)

egg chamber | germline clone

(Ferreira et al., 2014)

nurse cell | germline clone

(Ferreira et al., 2014)

Detailed Description

Statement

Reference

Females with Pkn^1T/Pkn^1T germline clones show no significant difference in total number of eggs or egg laying distribution compared to controls, but egg length is significantly reduced (though dorsal appendages are usually normally positioned).

During nurse cell dumping, egg chambers mutant for Pkn^1T/Pkn^1T show increased nurse cell-to-oocyte membrane collapse, leading to abnormal presence of ring canals, border cells and/or nurse cell nuclei in the oocyte cytoplasm.

Nurse cell adherens junctions and somatic follicular epithelial cells look normal, and no significant morphological defects are detected in ring canals or nurse cell cytoplasmic actin cables in Pkn^1T/Pkn^1T mutant germline clone egg chambers during early oogenesis. In mid-oogenesis there is an increase in the frequency of nurse cell-to-nurse cell membrane collapse (leading to multinucleated nurse cells after stage 6/7) in Pkn^1T/Pkn^1T mutant germline clone egg chambers.

Females with Pkn^1T/Pkn^1T germline clones show high abnormal accumulation of F-actin and myosin in nurse cells of stage 10 egg chambers, with an abnormally thick activated actin-myosin cytoskeleton with significant discontinuities that frequently radiates outwardly perpendicular to the anterior-posterior axis of the egg chamber.

Average nurse cell slow dumping rates in egg chambers from freshly dissected ovaries of Pkn^1T/Pkn^1T germline clones are not significantly different to controls, whereas fast dumping rates are significantly slower than controls.

(Ferreira et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Pkn^1T has egg | germline clone phenotype, enhanceable by sqh^{RLC.GFP(S65T)}/sqh^{RLC.GFP(S65T)}

(Ferreira et al., 2014)

Pkn^1T has egg | germline clone phenotype, enhanceable by sqh^E20.E21/sqh[+]

(Ferreira et al., 2014)

Pkn^1T has egg | germline clone phenotype, enhanceable by flw[+]/flw^G0172

(Ferreira et al., 2014)

NOT Enhanced by

Statement

Reference

Pkn^1T has egg | germline clone phenotype, non-enhanceable by Scer\GAL4^nanos.PU/Rok^{UAS.cUa.Tag:HA}

(Ferreira et al., 2014)

Pkn^1T has egg | germline clone phenotype, non-enhanceable by flw[+]/flw¹

(Ferreira et al., 2014)

Suppressed by

Statement

Reference

Pkn^1T has egg | germline clone phenotype, suppressible | partially by cpb^M143/cpb[+]

(Ferreira et al., 2014)

Pkn^1T has nurse cell | germline clone phenotype, suppressible | partially by cpb^M143/cpb[+]

(Ferreira et al., 2014)

Enhancer of

Statement

Reference

Pkn^1T/Pkn^1T is an enhancer of egg phenotype of sqh^E20.E21

(Ferreira et al., 2014)

NOT Enhancer of

Statement

Reference

Pkn^1T/Pkn^1T is a non-enhancer | germline clone of egg phenotype of Rok^{UAS.cUa.Tag:HA}, Scer\GAL4^nanos.PU

(Ferreira et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Presence of cpb^M143/+ partially suppresses the shorter egg length and nurse cell F-actin accumulation seen in Pkn^1T/Pkn^1T germline clone egg chambers.

sqh^{RLC.T:Avic\GFP-S65T}/sqh^{RLC.T:Avic\GFP-S65T} or sqh^E20.E21/+ or flw^G0172/+ enhances the shorter egg length phenotype seen in Pkn^1T/Pkn^1T germline clones.

Overexpression of Rok^{Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^nos.PU, or flw¹/+, does not enhance the shorter egg length phenotype seen in Pkn^1T/Pkn^1T germline clones.

The shorter egg length phenotype seen in females with overexpression of Rok^{Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^nos.PU is not enhanced in Pkn^1T/Pkn^1T germline clones.

(Ferreira et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

A5-44

(Ferreira et al., 2014)

Pkn^1T

pkn^1T

(Ferreira et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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