FlyBase Allele Report: Dmel\TBPH[Δ142]

General Information

Symbol

Dmel\TBPH^Δ142

Species

D. melanogaster

Name

FlyBase ID

FBal0221489

Feature type

allele

Associated gene

Dmel\TBPH

Associated Insertion(s)

P{?EPgy2}TBPH^Δ142

Carried in Construct

Also Known As

dTDP-43^Δ142

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Feiguin et al., 2009)

Mutagen

Delta2-3 transposase

(Feiguin et al., 2009)

Progenitor genotype

P{EPgy2}TBPH^EY10530

(Feiguin et al., 2009)

Associated Insertion(s)

P{?EPgy2}TBPH^Δ142

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a deletion of genomic DNA from coordinates 2R:19749289-19750093 . A portion of the original insertion remains.

(Feiguin et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

2R:23,861,766..23,862,570 [-]

Comment:

An 805 bp deletion resulting from the excision of P{EPgy2}TBPHEY10530. A 1138bp piece of the P element remains at the excision site.

(Feiguin et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of amyotrophic lateral sclerosis type 10

CEA with TBPH^Δ23

(Vanden Broeck et al., 2015)

CEA

(Romano et al., 2021, Romano et al., 2018)

model of neurodegenerative disease

CEA

(Feiguin et al., 2009)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates amyotrophic lateral sclerosis

modeled by Hsap\TARDBP^UAS.cHa

(Zhan et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult CCAP neuron (with TBPH^Δ23)

(Vanden Broeck et al., 2015)

anterior postalar bristle | increased number

(Li et al., 2013)

anterior postalar bristle | increased number (with TBPH^Q367X)

(Li et al., 2013)

dorsocentral bristle | increased number

(Li et al., 2013)

dorsocentral bristle | increased number (with TBPH^Q367X)

(Li et al., 2013)

embryonic/larval neuromuscular junction

(Feiguin et al., 2009)

embryonic/larval neuromuscular junction | second instar larval stage

(Godena et al., 2011)

embryonic/larval neuromuscular junction | third instar larval stage

(Godena et al., 2011)

mechanosensory neuron | increased number (with TBPH^Q367X)

(Li et al., 2013)

microtubule | third instar larval stage

(Godena et al., 2011)

NMJ bouton | second instar larval stage

(Godena et al., 2011)

NMJ bouton | third instar larval stage

(Godena et al., 2011)

scutellar bristle | increased number

(Li et al., 2013)

scutellar bristle | increased number (with TBPH^Q367X)

(Li et al., 2013)

synapse (with TBPH^Δ23)

(Miskiewicz et al., 2014)

synaptic vesicle (with TBPH^Δ23)

(Miskiewicz et al., 2014)

t-bar (with TBPH^Δ23)

(Miskiewicz et al., 2014)

type I bouton | third instar larval stage

(Feiguin et al., 2009)

Detailed Description

Statement

Reference

TBPH^Δ142/TBPH^Δ23 flies have significantly fewer CCAP/bursicon neurons (counted in the ventral nerve cord) than controls.

(Vanden Broeck et al., 2015)

TBPH^Δ23/TBPH^Δ142 exhibit an increase in the top size of synaptic T-bars and an increase in the number of tethered vesicles. The EJC amplitude and quantal content is not affected in these mutants.

(Miskiewicz et al., 2014)

A majority of TBPH^Δ142/TBPH^Δ142 mutant adults (with higher penetrance in females than males) display supernumerary anterior postalar bristles, a small fraction show supernumerary scutellar or supernumerary dorsocentral bristles.

A subset of TBPH^Q367X/TBPH^Δ142 mutant adults (with higher penetrance in females than males) display supernumerary anterior postalar bristles (most common phenotype when reared at 18[o]C or 25[o]C), supernumerary scutellar bristles (most common phenotype when reared at 30[o]C) or, in a small fraction of cases, supernumerary dorsocentral bristles. supernumerary bristles in mutants are accompanied by supernumerary sensory neurons. In general, supernumerary bristles are observed more frequently when flies are grown at 18[o]C than when reared at 25[o]C or 30[o]C.

(Li et al., 2013)

The NMJ morphology of TBPH^Δ142 mutant first instar larvae is similar to wild type. Presynaptic ramifications and muscular insertions appear normal in the mutant motor axons. In contrast, significant abnormalities are observed in second instar larval NMJs, and these are even more evident at the third instar larval stage. Muscle development appears normal in TBPH^Δ142 mutant flies: no differences in organ growth, cytoskeletal organisation or postsynaptic differentiation are observed.

TBPH^Δ142 mutant synaptic boutons on muscles 6 and 7 in third instar larvae appear deformed and are irregularly spaced along the terminal axons with several fused or elongated silhouettes and clear loss of their characteristic round-smooth shape. Microtubule stability is significantly reduced in the distal boutons compared to controls.

(Godena et al., 2011)

Homozygotes survive embryogenesis and more than 60% reach the pupal stage. However, only 16% of homozygotes eclose, with 32% becoming trapped in the pupal case. Homozygous adults have dramatic locomotive defects, in both walking and climbing assays.

Homozygous adults have a reduced lifespan compared to controls.

The number of type 1b and 1s boutons and the number of synaptic branches at the neuromuscular junction of muscles 6 and 7 is reduced in homozygous larvae compared to controls.

(Feiguin et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

TBPH^Δ142/TBPH^Δ23 has lethal - all die before end of P-stage phenotype, suppressible | partially by Scer\GAL4^nSyb.PU/Hsap\TARDBP^{G287S.UAS.cVBa}

(Vanden Broeck et al., 2015)

TBPH^Δ142/TBPH^Δ23 has lethal - all die before end of P-stage phenotype, suppressible | partially by Hsap\TARDBP^{A315T.UAS.cVBa}/Scer\GAL4^nSyb.PU

(Vanden Broeck et al., 2015)

TBPH^Δ142/TBPH^Δ23 has lethal - all die before end of P-stage phenotype, suppressible | partially by Scer\GAL4^nSyb.PU/Hsap\TARDBP^{D169G.UAS.cVBa}

(Vanden Broeck et al., 2015)

TBPH^Δ142/TBPH^Δ23 has lethal - all die before end of P-stage phenotype, suppressible | partially by Scer\GAL4^nSyb.PU/Hsap\TARDBP^{A90V.UAS.cVBa}

(Vanden Broeck et al., 2015)

TBPH^Δ142/TBPH^Δ23 has lethal - all die before end of P-stage phenotype, suppressible | partially by Hsap\TARDBP^UAS.cVBa/Scer\GAL4^nSyb.PU

(Vanden Broeck et al., 2015)

TBPH^Δ142/TBPH^Δ23 has decreased cell number phenotype, suppressible by Scer\GAL4^nSyb.PU/Hsap\TARDBP^{D169G.UAS.cVBa}

(Vanden Broeck et al., 2015)

TBPH^Δ142/TBPH^Δ23 has decreased cell number phenotype, suppressible by Hsap\TARDBP^UAS.cVBa/Scer\GAL4^nSyb.PU

(Vanden Broeck et al., 2015)

TBPH^Δ142/TBPH^Δ23 has lethal - all die before end of pupal stage phenotype, suppressible by Scer\GAL4^nSyb.PU/Map205^GD8458

(Vanden Broeck et al., 2013)

NOT suppressed by

Statement

Reference

TBPH^Δ142/TBPH^Δ23 has partially lethal - majority die phenotype, non-suppressible by Df(3R)Xrp1-Plus/+

(Mallik et al., 2018)

TBPH^Δ142/TBPH^Δ23 has decreased cell number phenotype, non-suppressible by Scer\GAL4^nSyb.PU/Hsap\TARDBP^{G287S.UAS.cVBa}

(Vanden Broeck et al., 2015)

TBPH^Δ142/TBPH^Δ23 has decreased cell number phenotype, non-suppressible by Scer\GAL4^nSyb.PU/Hsap\TARDBP^{A90V.UAS.cVBa}

(Vanden Broeck et al., 2015)

TBPH^Δ142/TBPH^Δ23 has decreased cell number phenotype, non-suppressible by Hsap\TARDBP^{A315T.UAS.cVBa}/Scer\GAL4^nSyb.PU

(Vanden Broeck et al., 2015)

Suppressor of

Statement

Reference

TBPH^Δ142/TBPH[+] is a suppressor of short lived phenotype of Hsap\TARDBP^UAS.cHa, Scer\GAL4^Toll-6-D42

(Zhan et al., 2013)

Other

Statement

Reference

Hsap\TARDBP^{A315T.UAS.cVBa}, Scer\GAL4^nSyb.PU, TBPH^Δ142/TBPH^Δ23 has short lived phenotype

(Vanden Broeck et al., 2015)

Hsap\TARDBP^{A90V.UAS.cVBa}, Scer\GAL4^nSyb.PU, TBPH^Δ142/TBPH^Δ23 has short lived phenotype

(Vanden Broeck et al., 2015)

Hsap\TARDBP^{D169G.UAS.cVBa}, Scer\GAL4^nSyb.PU, TBPH^Δ142/TBPH^Δ23 has short lived phenotype

(Vanden Broeck et al., 2015)

Hsap\TARDBP^{G287S.UAS.cVBa}, Scer\GAL4^nSyb.PU, TBPH^Δ142/TBPH^Δ23 has short lived phenotype

(Vanden Broeck et al., 2015)

Hsap\TARDBP^UAS.cVBa, Scer\GAL4^nSyb.PU, TBPH^Δ142/TBPH^Δ23 has short lived phenotype

(Vanden Broeck et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

TBPH^Δ142/TBPH^Δ23 has adult CCAP neuron phenotype, suppressible by Scer\GAL4^nSyb.PU/Hsap\TARDBP^{D169G.UAS.cVBa}

(Vanden Broeck et al., 2015)

TBPH^Δ142/TBPH^Δ23 has adult CCAP neuron phenotype, suppressible by Hsap\TARDBP^UAS.cVBa/Scer\GAL4^nSyb.PU

(Vanden Broeck et al., 2015)

NOT suppressed by

Statement

Reference

TBPH^Δ142/TBPH^Δ23 has adult CCAP neuron phenotype, non-suppressible by Scer\GAL4^nSyb.PU/Hsap\TARDBP^{G287S.UAS.cVBa}

(Vanden Broeck et al., 2015)

TBPH^Δ142/TBPH^Δ23 has adult CCAP neuron phenotype, non-suppressible by Scer\GAL4^nSyb.PU/Hsap\TARDBP^{A90V.UAS.cVBa}

(Vanden Broeck et al., 2015)

TBPH^Δ142/TBPH^Δ23 has adult CCAP neuron phenotype, non-suppressible by Hsap\TARDBP^{A315T.UAS.cVBa}/Scer\GAL4^nSyb.PU

(Vanden Broeck et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Map205^GD8458 under the control of Scer\GAL4^nSyb.PU suppresses the late pupal lethality seen in TBPH^Δ142/TBPH^Δ23 mutants.

(Vanden Broeck et al., 2013)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\TARDBP^{G287S.Scer\UAS.cVBa}, Hsap\TARDBP^{A315T.Scer\UAS.cVBa}, Hsap\TARDBP^{D169G.Scer\UAS.cVBa}, Hsap\TARDBP^{A90V.Scer\UAS.cVBa} or Hsap\TARDBP^{Scer\UAS.cVBa} driven by Scer\GAL4^nSyb.PU equally partially rescue pupal lethality (around 50% of flies expected by a full rescue eclose) in TBPH^Δ142/TBPH^Δ23 mutants. However, the median lifespan of surviving TBPH^Δ142/TBPH^Δ23 flies with expression of Hsap\TARDBP^{A90V.Scer\UAS.cVBa}, Hsap\TARDBP^{A315T.Scer\UAS.cVBa} or Hsap\TARDBP^{G287S.Scer\UAS.cVBa} is significantly lower than flies with expression of Hsap\TARDBP^{D169G.Scer\UAS.cVBa} or Hsap\TARDBP^{Scer\UAS.cVBa} (driven by Scer\GAL4^nSyb.PU); all genotypes display shorter lifespan than controls. Expression (driven by Scer\GAL4^nSyb.PU) of Hsap\TARDBP^{D169G.Scer\UAS.cVBa} or Hsap\TARDBP^{Scer\UAS.cVBa} (but not Hsap\TARDBP^{A90V.Scer\UAS.cVBa}, Hsap\TARDBP^{A315T.Scer\UAS.cVBa} or Hsap\TARDBP^{G287S.Scer\UAS.cVBa}) suppresses the loss of bursicon neurons seen in TBPH^Δ142/TBPH^Δ23 mutants.

(Vanden Broeck et al., 2015)

Heterozygous TBPH^Δ142 causes a small increase in the lifespan of Scer\GAL4^D42>Hsap\TARDBP^Scer\UAS.cHa flies.

(Zhan et al., 2013)

Complementation and Rescue Data

Rescued by

TBPH^Q367X/TBPH^Δ142 is rescued by Scer\GAL4^vg.PU/TBPH^UAS.cLu

(Li et al., 2013)

TBPH^Δ142 is rescued by TBPH^UAS.Tag:FLAG/Scer\GAL4^Toll-6-D42

(Godena et al., 2011)

Comments

Expression of TBPH^Scer\UAS.cLu under the control of Scer\GAL4^vg.PU rescues the supernumerary anterior postalar bristle phenotype of TBPH^Q367X/TBPH^Δ142 mutants.

(Li et al., 2013)

Expression of TBPH^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^D42 rescues the defects in microtubule stability seen in TBPH^Δ142 mutant distal boutons.

(Godena et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

93600

w^*; TBPH^Δ142/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

TBPH^D142

(Godena et al., 2011)

TBPH^Δ142

(Pandey et al., 2025, Strah et al., 2020, Mallik et al., 2018, Romano et al., 2014, Llamusi et al., 2013, Zhan et al., 2013, Feiguin et al., 2009)

TBPH^Δ142T

(Vanden Broeck et al., 2015)

dTDP-43^Δ142

(Li et al., 2013, Vanden Broeck et al., 2013)

tbph^Δ142

(Gbadamosi et al., 2025, Romano et al., 2021, Romano et al., 2018, Miskiewicz et al., 2014)

Δ142

(Hazelett et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (16)

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