FlyBase Allele Report: Dmel\nudE[39A]

FB2026_02 , released June 18, 2026

FB2026_02 , released June 18, 2026

Allele: Dmel\nudE^39A

General Information

Symbol

Dmel\nudE^39A

Species

D. melanogaster

Name

FlyBase ID

FBal0221533

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Wainman et al., 2009)

Mutagen

P-element activity

(Wainman et al., 2009)

Progenitor genotype

P{EPgy2}EY09537

(Wainman et al., 2009)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a deletion that removes the first three exons and part of the fourth exon of nudE. This deletion removes the first 37 codons of nudE, including the initiation codon.

(Wainman et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

axon | third instar larval stage

(Arthur et al., 2015)

axon | third instar larval stage (with Df(3L)BSC673)

(Arthur et al., 2015)

(Wainman et al., 2009)

centrosome & spermatocyte

(Wainman et al., 2009)

centrosome | increased number

(Wainman et al., 2009)

condensed chromosome

(Wainman et al., 2009)

condensed chromosome (with Df(3L)AC1)

(Wainman et al., 2009)

dendrite | third instar larval stage

(Arthur et al., 2015)

dendrite | third instar larval stage (with Df(3L)BSC673)

(Arthur et al., 2015)

larval multidendritic class IV neuron | third instar larval stage

(Arthur et al., 2015)

larval multidendritic class IV neuron | third instar larval stage (with Df(3L)BSC673)

(Arthur et al., 2015)

microtubule | third instar larval stage

(Arthur et al., 2015)

(Wainman et al., 2009)

nucleus & onion stage spermatid

(Wainman et al., 2009)

oocyte | germline clone

(Hayashi et al., 2014)

(Wainman et al., 2009)

spindle & primary spermatocyte in meiotic metaphase I

(Wainman et al., 2009)

(Wainman et al., 2009)

Detailed Description

Statement

Reference

nudE^39A/nudE^39A or nudE^39A/Df(3L)BSC673 third instar larval class IV da neurons show significant decreases in dendrite length and branch number compared to controls; mutant neurons also display a severe axon splitting phenotype (multiple fine branches form a short distance from the soma, rather than extending as a single process). The number of Golgi complexes mislocalizing to axons significantly increases in nudE^39A/nudE^39A larvae. nudE^39A/nudE^39A regulates microtubule growth and dynamics in both axons and dendrites (significant increase in Eb1 comets), as well as orientation of axonal microtubules (rather than wild type purely anterograde movement, mutant microtubules show anterograde and retrograde movement).

(Arthur et al., 2015)

Homozygous germline clones result in a failure to specify the oocyte.

(Hayashi et al., 2014)

Spermatids from mutant third instar larvae testes do not show cytokinesis defects.

(Wainman et al., 2012)

The mitotic progression of many cells in homozygous and nudE^39A/Df(3L)AC1 larval brains is arrested at prometaphase or metaphase, with very few cells continuing to anaphase. The mitotic index is substantially elevated compared to that of wild type. 89.4% of homozygous mitotic cells and 96.9% of nudE^39A/Df(3L)AC1 mitotic cells have overcondensed chromosomes.

Only a small minority of mitotic cells have normal (bipolar, straight) spindles in homozygous larval brains, with the most frequent defects being broad, unfocused spindle poles and the failure of centrosomes to remain attached to the spindles. Other defects include curved spindles, the presence of two centrosomes at one pole but none at the other pole, and supernumerary centrosomes.

The chromosomes fail to form a tight metaphase plate in most mitotic figures of homozygous larval brains. 61% of mutant cells have uncongressed chromosomes compared to only 42% in control cells.

The centrosomes fail to move from the cortex to the nuclear envelope just before the first meiotic division in homozygous spermatocytes, in contrast to wild type. 88% of cells at meiosis metaphase I in homozygous testes show spindle-detachment defects. The mutant metaphase I spermatocytes often have spindle-like bipolar arrays of microtubules which are assembled around the chromosomes but not connected to the membrane-bound asters.

The proportions of cells in meiotic anaphase or telophase are normal in mutant testes, and there is no failure in cytokinesis. However, chromosome segregation is often aberrant at late anaphase and telophase, with DNA apportioned unequally to the two daughter cells or with DNA remaining in the middle of the spindle. As a result, mutant onion stage spermatids have nuclei of different sizes. In addition, the sizes of the Nebenkern is uneven.

(Wainman et al., 2009)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

nudE^39A has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Lis-1^UAS.cLa/Scer\GAL4^ppk.PU

(Arthur et al., 2015)

nudE^39A has abnormal mitotic cell cycle phenotype, suppressible | partially by Zwilch¹²²⁹/Zwilch¹²²⁹

(Wainman et al., 2009)

Enhancer of

Statement

Reference

nudE^39A/nudE^39A is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Dlic^RNAi.UAS.cUa, Scer\GAL4^ppk.PU

(Arthur et al., 2015)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

nudE^39A has microtubule | third instar larval stage phenotype, non-enhanceable by γTub23C^A14-9/γTub23C^A15-2

(Arthur et al., 2015)

Suppressed by

Statement

Reference

nudE^39A has dendrite | third instar larval stage phenotype, suppressible by Lis-1^UAS.cLa/Scer\GAL4^ppk.PU

(Arthur et al., 2015)

Enhancer of

Statement

Reference

nudE^39A/nudE^39A is an enhancer of larval multidendritic class IV neuron | third instar larval stage phenotype of Dlic^RNAi.UAS.cUa, Scer\GAL4^ppk.PU

(Arthur et al., 2015)

nudE^39A/nudE^39A is an enhancer of dendrite | third instar larval stage phenotype of Dlic^RNAi.UAS.cUa, Scer\GAL4^ppk.PU

(Arthur et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

nudE^39A/nudE^39A enhances dendrite morphology defects in class IV da neurons of third instar larvae with expression of Dlic^{dsRNA.Scer\UAS.cUa} (along with UAS-dcr2) driven by Scer\GAL4^ppk.PU.

γTub23C^A15-2/γTub23C^A14-9 does not affect the amount of axonal microtubule retrograde transport seen in nudE^39A/nudE^39A class IV da neurons in third instar larvae.

Expression of Lis-1^Scer\UAS.cLa driven by Scer\GAL4^ppk.PU significantly suppresses decreases in dendrite length, branch number and distribution seen in nudE^39A/nudE^39A third instar larval ddaC neurons.

(Arthur et al., 2015)

The mitotic index and the proportion of mitotic figures in anaphase relative to those in prometaphase or metaphase is nearly normal in the brains of nudE^39A zwilch¹²²⁹ double homozygous larvae.

(Wainman et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

nudE^39A is rescued by nudE^ΔC

(Arthur et al., 2015)

Df(3L)BSC673/nudE^39A is rescued by nudE^+tAa

(Arthur et al., 2015)

nudE^39A is rescued by nudE^ΔC.UAS/Scer\GAL4^ppk.PU

(Arthur et al., 2015)

Partially rescued by

Df(3L)BSC673/nudE^39A is partially rescued by nudE^E113A.R124A

(Arthur et al., 2015)

nudE^39A is partially rescued by Scer\GAL4^ppk.PU/nudE^UAS.A

(Arthur et al., 2015)

Comments

nudE^+tAa rescues nudE^39A/Df(3L)BSC673 lethality, with morphologically normal adults apart from slightly disordered wing margin bristles; decreases in dendrite length and branch number and the splitting axon phenotype in third instar larval class IV da neurons are rescued. Expression of nudE^Scer\UAS.A driven by Scer\GAL4^ppk.PU partially rescues the decreases in dendrite length and branch number and the axon splitting phenotype (but not lethality) in nudE^39A/nudE^39A third instar larval class IV da neurons. Expression of nudE^ΔC.Scer\UAS driven by Scer\GAL4^ppk.PU fully rescues decreases in dendrite length and branch number in nudE^39A/nudE^39A third instar larval class IV da neurons (significantly better rescue compared to nudE^Scer\UAS.A). nudE^ΔC fully rescues decreases in dendrite length and branch number in nudE^39A/nudE^39A third instar larval class IV da neurons. nudE^E113A.R124A partially rescues decreases in dendrite length and branch number in nudE^39A/Df(3L)BSC673 third instar larval ddaC neurons.

(Arthur et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Df(3L)nudE^39A

(Wainman et al., 2009)

nudE^39A

(Arthur et al., 2015, Hayashi et al., 2014, Wainman et al., 2009)

nudE^39E

(Wainman et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections