FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Hrb98DEd05172
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General Information
Symbol
Dmel\Hrb98DEd05172
Species
D. melanogaster
Name
FlyBase ID
FBal0227018
Feature type
allele
Associated gene
Dmel\Hrb98DE
Associated Insertion(s)
P{XP}Hrb98DEd05172
Carried in Construct
Key Links
Genomic Maps

Allele class

hypomorphic allele - molecular evidence

Mutagen
    Nature of the Allele
    Allele class

    hypomorphic allele - molecular evidence

    (Ji and Tulin, 2012)
    Mutagen
    Progenitor genotype
    Associated Insertion(s)
    P{XP}Hrb98DEd05172
    Cytology
    Description
    Allele components
    Component
    Use(s)
    Inserted element
    P{XP}
    Regulatory region(s)
    UAS
    Also carries
    FRT
    Mutations Mapped to the Genome
    Curation Data
    Variant Molecular Consequences
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference

        Hrb98DEd05172 is homozygous lethal.

        Three-fourths of the Hrb98DEd05172/Df(3R)Exel6209 hemizygotes die before the pupal stage.

        Hemizygous Hrb98DEd05172/Df(3R)Exel6209 female escapers have very low fertility and egg laying rates compared with wild-type.

        Hrb98DEd05172/Df(3R)Exel6209 mutant females display defects during egg development, including fused egg chambers and mislocalisation of the oocyte. Mispositioned oocytes in the Hrb98DEd05172/Df(3R)Exel6209 mutants are found either at the anterior pole or the lateral side of the egg chamber. Wild-type controls show none of these defects.

        Similar to the wild-type, most germaria of Hrb98DEd05172/Df(3R)Exel6209 mutants have two or three germline stem cells (GSCs) at day 3 of eclosion. At day 10, wild-type germaria have two or three GSCs, although most germaria in the Hrb98DEd05172/Df(3R)Exel6209 mutant have only one GSC left. At 17 days after eclosion, about 70% of germaria in the Hrb98DEd05172/Df(3R)Exel6209 mutants loose their GSCs, whereas the wild-type still retains GSC self-renewal ability.

        (Ji and Tulin, 2012)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Phenotype Manifest In
        Additional Comments
        Genetic Interactions
        Statement
        Reference

        Expression of shgScer\UAS.P\T.cJa in the germline under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 is sufficient to restore normal oocyte localisation in Hrb98DEd05172/Df(3R)Exel6209 mutants. shgScer\UAS.P\T.cJa-expression in the germline suppresses all the oocyte and egg chamber abnormalities associated with Hrb98DEd05172/Df(3R)Exel6209.

        Parg27.1/+ enhances the oocyte-mislocalisation phenotype of Hrb98DEd05172/Df(3R)Exel6209 mutants.

        Expression of Hrb98DEScer\UAS.P\T.T:Disc\RFP in the germline under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 fully rescues the oocyte mislocalisation in Parg27.1/+; Hrb98DEd05172/Df(3R)Exel6209 mutants.

        Expression of shgScer\UAS.P\T.cJa in the germline under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 fully rescues the oocyte mislocalisation in Parg27.1/+; Hrb98DEd05172/Df(3R)Exel6209 mutants.

        Ectopic expression of shgScer\UAS.P\T.cJa in the germline under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 significantly increased the germline stem cell self-renewal ability of Hrb98DEd05172/Df(3R)Exel6209 mutants.

        Parg27.1/+ causes a more rapid germline stem cell loss in Hrb98DEd05172/Df(3R)Exel6209 mutants.

        Expression of Hrb98DEScer\UAS.P\T.T:Disc\RFP in the germline under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 fully restores the germline stem cell maintenance ability in Parg27.1/+; Hrb98DEd05172/Df(3R)Exel6209 mutants.

        Expression of shgScer\UAS.P\T.cJa in the germline under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 fully restores the germline stem cell maintenance ability in Parg27.1/+; Hrb98DEd05172/Df(3R)Exel6209 mutants.

        (Ji and Tulin, 2012)
        Xenogenetic Interactions
        Statement
        Reference
        Complementation and Rescue Data
        Rescued by

        Hrb98DEd05172/Df(3R)Exel6209 is rescued by Scer\GAL4arm.PS/Hrb98DEUAS.RFP(Unk)

        (Ji and Tulin, 2012)

        Hrb98DEd05172/Df(3R)Exel6209 is rescued by Scer\GAL4VP16.nanos.UTR/Hrb98DEUASp.RFP(Unk)

        (Ji and Tulin, 2012)
        Comments

        Expression of Hrb98DEScer\UAS.P\T.T:Disc\RFP in germline cells under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 fully rescues the defective phenotypes of Hrb98DEd05172/Df(3R)Exel6209 hemizygotes.

        Ubiquitous expression of Hrb98DEScer\UAS.T:Disc\RFP under the control of Scer\GAL4arm.PS is sufficient to rescue the lethality of Hrb98DEd05172/Df(3R)Exel6209 hemizygotes.

        (Ji and Tulin, 2012)
        Images (0)
        Mutant
        Wild-type
        Stocks (1)
        Notes on Origin
        Discoverer
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (2)
        Reported As
        Symbol Synonym
        Hrb98DEd05172
        Hrp38d05172
        (Ustaoglu et al., 2019)
        Name Synonyms
        Secondary FlyBase IDs
          References (4)