FlyBase Allele Report: Dmel\Marf[UAS.Tag:FLAG]

General Information

Symbol

Dmel\Marf^UAS.Tag:FLAG

Species

D. melanogaster

Name

FlyBase ID

FBal0230288

Feature type

allele

Associated gene

Dmel\Marf

Associated Insertion(s)

Carried in Construct

P{UAS-Marf.Flag}

Also Known As

UAS-Marf, UAS-Marf-FLAG

Key Links

Transgenic product class

wild_type

(Park et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Park et al., 2009)

Mutagen

in vitro construct

(Park et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-Marf.Flag}

Cytology

Description

UAS regulatory sequences drive expression of Marf, which is tagged at the C-terminal end with Tag:FLAG.

(Park et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Marf

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^ple.PF

(Hwang et al., 2014)

chemical resistant | adult stage, with Scer\GAL4^ple.PF

(Hwang et al., 2014)

visible, with Scer\GAL4^GMR.PU

(López Del Amo et al., 2015)

Phenotype Manifest In

axon | adult stage, with Scer\GAL4^GMR15B07

(Zhang et al., 2025)

dopaminergic PPL1 neuron | adult stage, with Scer\GAL4^ple.PF

(Hwang et al., 2014)

eye, with Scer\GAL4^GMR.PU

(López Del Amo et al., 2015)

gustatory receptor neuron of the wing, with Scer\GAL4^GMR15B07

(Zhang et al., 2025)

indirect flight muscle cell, with Scer\GAL4^Mef2.PR

(Park et al., 2010)

interommatidial bristle, with Scer\GAL4^GMR.PU

(López Del Amo et al., 2015)

mitochondrion, with Scer\GAL4^Mef2.PR

(Park et al., 2010)

mitochondrion & dopaminergic neuron, with Scer\GAL4^ple.PF

(Park et al., 2009)

mitochondrion | adult stage, with Scer\GAL4^GMR15B07

(Zhang et al., 2025)

mitochondrion | adult stage, with Scer\GAL4^ple.PF

(Hwang et al., 2014)

ommatidium, with Scer\GAL4^GMR.PU

(López Del Amo et al., 2015)

Detailed Description

Statement

Reference

Expression of Marf^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PU results in a severe rough eye phenotype (ommatidia cannot be discerned and interommatidial bristles are missing).

(López Del Amo et al., 2015)

Expression of Marf^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^ple.PF leads to protection from rotenone toxicity, with mutant flies exhibiting no significant loss of PPL1 dopaminergic neurons in adult brains after rotenone feeding, in contrast to controls.

Dopaminergic neurons in flies expressing Marf^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^ple.PF exhibit mitochondrial aggregation and fusion, as compared to controls.

Flies expressing Marf^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PU do not exhibit any discernible eye phenotype, as compared to controls.

(Hwang et al., 2014)

Adults expressing Marf^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Mef2.PR show defects in mitochondrial morphology in the indirect flight muscles, with larger than normal mitochondria being seen between the muscle fibers.

(Park et al., 2010)

Expression of Marf^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^ple.PF results in rounder and bigger mitochondria than normal in the dopaminergic neurons.

(Park et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Marf^UAS.Tag:FLAG, Scer\GAL4^GMR.PU has visible phenotype, suppressible | partially by Gdap1^UAS.cLa, Scer\GAL4^GMR.PU

(López Del Amo et al., 2015)

Other

Statement

Reference

Hsap\UCP2^UAS.cFa, Marf^UAS.Tag:FLAG, Scer\GAL4^GMR.PU has visible phenotype

(Hwang et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Marf^UAS.Tag:FLAG, Scer\GAL4^GMR15B07 has mitochondrion | adult stage phenotype, enhanceable by Mtx2^5'.sgRNA/Spyo\Cas9^UAS.P2, Scer\GAL4^GMR15B07

(Zhang et al., 2025)

Marf^UAS.Tag:FLAG, Scer\GAL4^GMR15B07 has gustatory receptor neuron of the wing phenotype, enhanceable by Mtx2^5'.sgRNA/Spyo\Cas9^UAS.P2, Scer\GAL4^GMR15B07

(Zhang et al., 2025)

Marf^UAS.Tag:FLAG, Scer\GAL4^GMR15B07 has axon | adult stage phenotype, enhanceable by Mtx2^5'.sgRNA/Spyo\Cas9^UAS.P2, Scer\GAL4^GMR15B07

(Zhang et al., 2025)

Suppressed by

Statement

Reference

Marf^UAS.Tag:FLAG, Scer\GAL4^GMR.PU has eye phenotype, suppressible | partially by Gdap1^UAS.cLa, Scer\GAL4^GMR.PU

(López Del Amo et al., 2015)

Marf^UAS.Tag:FLAG, Scer\GAL4^GMR.PU has interommatidial bristle phenotype, suppressible | partially by Gdap1^UAS.cLa, Scer\GAL4^GMR.PU

(López Del Amo et al., 2015)

Marf^UAS.Tag:FLAG, Scer\GAL4^GMR.PU has ommatidium phenotype, suppressible | partially by Gdap1^UAS.cLa, Scer\GAL4^GMR.PU

(López Del Amo et al., 2015)

Other

Statement

Reference

Hsap\UCP2^UAS.cFa, Marf^UAS.Tag:FLAG, Scer\GAL4^GMR.PU has eye phenotype

(Hwang et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Gdap1^Scer\UAS.cLa partially rescues the rough eye phenotype caused by expression of Marf^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PU (ommatidia can be observed and the number of interommatidial bristles is increased).

(López Del Amo et al., 2015)

Co-expression of Marf^{Scer\UAS.T:Zzzz\FLAG} significantly rescues the defects in mitochondrial morphology in the indirect flight muscles which is seen in adults expressing porin^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Mef2.PR.

(Park et al., 2010)

The downturned wing, crushed thorax and flightless phenotypes of Pink1^B9 mutant flies are not suppressed by expression of Marf^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^hs.PB.

(Park et al., 2009)

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\UCP2^Scer\UAS.cFa and Marf^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PU results in a rough eye phenotype.

(Hwang et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Marf^{Scer\UAS.T:Zzzz\FLAG}

Marf^UAS.Tag:FLAG

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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