FlyBase Allele Report: Dmel\Npc1a[KK101077]

General Information

Symbol

Dmel\Npc1a^KK101077

Species

D. melanogaster

Name

FlyBase ID

FBal0231055

Feature type

allele

Associated gene

Dmel\Npc1a

Associated Insertion(s)

Carried in Construct

P{KK101077}

Also Known As

UAS-npc1a-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK101077}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK101077

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Niemann-Pick disease

CEA

(Danielsen et al., 2016)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Niemann-Pick disease

is ameliorated by Tsc1^UAS.cTa

(Danielsen et al., 2016)

is ameliorated by gig^UAS.cTa

(Danielsen et al., 2016)

is ameliorated by Atg1^UAS.cSa

(Danielsen et al., 2016)

is ameliorated by Atg13^UAS.cCa

(Danielsen et al., 2016)

exacerbates Parkinson's disease 1

modeled by Hsap\SNCA^UAS.cJa

(Yu et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with Scer\GAL4^P0206

(Danielsen et al., 2016)

abnormal developmental rate, with Scer\GAL4^phtm.PO

(Danielsen et al., 2016)

increased body size | pupal stage, with Scer\GAL4^P0206

(Danielsen et al., 2016)

Phenotype Manifest In

endosome, with Scer\GAL4^phtm.PO

(Danielsen et al., 2016)

prothoracic gland | third instar larval stage, with Scer\GAL4^P0206

(Danielsen et al., 2016)

prothoracic gland | third instar larval stage, with Scer\GAL4^phtm.PO

(Danielsen et al., 2016)

Detailed Description

Statement

Reference

Expression of Npc1a^KK101077 under the control of Scer\GAL4^phm.PO (using tub-Gal80[ts] to limit the time of expression) results in accumulation of lipid droplets in the prothoracic gland cells of third instar larvae as well as trafficking defects as suggested by enlarged Rab7 positive vesicles and Lamp1 marker accumulations.

Without the tub-Gal80[ts] limiting the onset of expression the animals show developmental arrest in first larval instar.

Expression of Npc1a^KK101077 RNAi under the Scer\GAL4^P0206 driver results in a significant delay in pupariation and increased pupal size. Third instar larvae show accumulations of lipid droplets in prothoracic gland cells.

(Danielsen et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Npc1a^KK101077, Scer\GAL4^phtm.PO has abnormal developmental rate phenotype, suppressible by sit^UAS.Tag:HA, Scer\GAL4^phtm.PO

(Danielsen et al., 2016)

Npc1a^KK101077, Scer\GAL4^P0206 has abnormal developmental rate phenotype, suppressible by Atg1^UAS.cSa/Atg13^UAS.cCa, Scer\GAL4^P0206

(Danielsen et al., 2016)

Npc1a^KK101077, Scer\GAL4^P0206 has increased body size | pupal stage phenotype, suppressible by Atg1^UAS.cSa/Atg13^UAS.cCa, Scer\GAL4^P0206

(Danielsen et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Npc1a^KK101077, Scer\GAL4^phtm.PO has prothoracic gland | third instar larval stage phenotype, suppressible by gig^UAS.cTa/Tsc1^UAS.cTa, Scer\GAL4^phtm.PO

(Danielsen et al., 2016)

Npc1a^KK101077, Scer\GAL4^P0206 has prothoracic gland | third instar larval stage phenotype, suppressible by gig^UAS.cTa/Tsc1^UAS.cTa, Scer\GAL4^P0206

(Danielsen et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The L1 developmental arrest observed in animals expressing Npc1a^KK101077 under the control of Scer\GAL4^phm.PO is rescued by co-expression of sit^{Scer\UAS.T:Ivir\HA1}.

The accumulation of lipid droplets observed in prothoracic gland cells of third instar larvae expressing Npc1a^KK101077 under the control of either Scer\GAL4^phm.PO or Scer\GAL4^P0206 is prevented by co-expression of Tsc1^Scer\UAS.cTa and gig^Scer\UAS.cTa.

The pupariation delay and increased pupal size observed in flies expressing Npc1a^KK101077 under the Scer\GAL4^P0206 driver is rescued by simultaneous co-expression of Atg1^Scer\UAS.cSa and Atg13^Scer\UAS.cCa.

(Danielsen et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v105405

P{KK101077}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Npc1a^KK101077

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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