FlyBase Allele Report: Dmel\Zasp52[KK101276]

General Information

Symbol

Dmel\Zasp52^KK101276

Species

D. melanogaster

Name

FlyBase ID

FBal0231166

Feature type

allele

Associated gene

Dmel\Zasp52

Associated Insertion(s)

Carried in Construct

P{KK101276}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK101276}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK101276

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates atypical teratoid rhabdoid tumor

modeled by Snr1^KK101602

(Tegeder et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

flightless, with Dcr-2^UAS.cDa, Scer\GAL4^Mef2.PR

(Katzemich et al., 2013)

flightless, with Scer\GAL4^Act88F.PB

(Chechenova et al., 2013)

flightless, with Scer\GAL4^Mef2.PR

(Katzemich et al., 2013)

some die during pupal stage, with Dcr-2^UAS.cDa, Scer\GAL4^Mef2.PR

(Katzemich et al., 2013)

Phenotype Manifest In

H zone, with Dcr-2^UAS.cDa, Scer\GAL4^Mef2.PR

(Katzemich et al., 2013)

H zone, with Scer\GAL4^Act88F.PB

(Katzemich et al., 2013)

H zone, with Scer\GAL4^Mef2.PR

(Katzemich et al., 2013)

indirect flight muscle cell, with Dcr-2^UAS.cDa, Scer\GAL4^Mef2.PR

(Katzemich et al., 2013)

indirect flight muscle cell, with Scer\GAL4^Act88F.PB

(Chechenova et al., 2013, Katzemich et al., 2013)

indirect flight muscle cell, with Scer\GAL4^Mef2.PR

(Katzemich et al., 2013)

M band, with Dcr-2^UAS.cDa, Scer\GAL4^Mef2.PR

(Katzemich et al., 2013)

myofibril, with Dcr-2^UAS.cDa, Scer\GAL4^Mef2.PR

(Katzemich et al., 2013)

myofibril, with Scer\GAL4^Act88F.PB

(Chechenova et al., 2013)

myofibril, with Scer\GAL4^Mef2.PR

(Katzemich et al., 2013)

Z disc, with Dcr-2^UAS.cDa, Scer\GAL4^Mef2.PR

(Katzemich et al., 2013)

Z disc, with Scer\GAL4^Act88F.PB

(Chechenova et al., 2013, Katzemich et al., 2013)

Detailed Description

Statement

Reference

Indirect flight muscle myofibril structure is aberrant in flies expressing Zasp52^KK101276 under the control of Scer\GAL4^Act88F.PB : the myofibrils show variations in thickness compared to wild type and Z discs are indistinct.

Females expressing Zasp52^KK101276 under the control of Scer\GAL4^Act88F.PB are flightless.

(Chechenova et al., 2013)

The indirect flight muscles (IFMs) of adults expressing Zasp52^KK101276 under the control of Scer\GAL4^Mef2.PR occasionally have bent H-zones and frayed myofibrils are visible. If Dcr-2^Scer\UAS.cDa is also present to enhance RNAi, the H-zones are severely distorted and the myofibrils are often frayed.

Defects in the myofibrils of the IFMs become apparent at 48 hours after puparium formation (APF) in animals expressing Zasp52^KK101276 under the control of Scer\GAL4^Mef2.PR in the presence of Dcr-2^Scer\UAS.cDa. At this stage the myofibrils are thinner than normal and are not well structured. At 72 hours APF some of the myofibrils are frayed and Z-discs and M-lines are deformed.

Adults expressing under Zasp52^KK101276 the control of Scer\GAL4^Act88F.PB have defects in the indirect flight muscles, with almost no Z-disc material being present and H-zones and Z-discs occasionally being distorted.

(Katzemich et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Zasp52^KK101276/Scer\GAL4^repo.PU is a suppressor | partially of lethal - all die during P-stage phenotype of Scer\GAL4^repo.PU, Snr1^KK101602

(Tegeder et al., 2019)

Other

Statement

Reference

Scer\GAL4^repo.PU, Snr1^KK101602, Zasp52^KK101276 has partially lethal - majority die phenotype

(Tegeder et al., 2019)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^Act88F.PB, Zasp52^KK101276 has H zone phenotype, enhanceable by Scer\GAL4^Act88F.PB/Zasp66^KK112973

(Katzemich et al., 2013)

Scer\GAL4^Act88F.PB, Zasp52^KK101276 has indirect flight muscle cell phenotype, enhanceable by Scer\GAL4^Act88F.PB/Zasp66^KK112973

(Katzemich et al., 2013)

Scer\GAL4^Act88F.PB, Zasp52^KK101276 has Z disc phenotype, enhanceable by Scer\GAL4^Act88F.PB/Zasp66^KK112973

(Katzemich et al., 2013)

Enhancer of

Statement

Reference

Zasp52^KK101276/Scer\GAL4^GMR.long is an enhancer of eye phenotype of Scer\GAL4^GMR.long, parvin^UASp.mGFP6

(Chountala et al., 2012)

Other

Statement

Reference

Scer\GAL4^Act88F.PB, Zasp52^KK101276, Zasp66^KK112973 has myofibril phenotype

(Katzemich et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Adults co-expressing Zasp52^KK101276 and Zasp66^KK112973 under the control of Scer\GAL4^Act88F.PB have severe defects in the indirect flight muscles. Myofibrils are often frayed and unstable, with severely distorted Z-discs and H-zones.

(Katzemich et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v106177

P{KK101276}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Zasp52^KK101276

(Chountala et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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