FlyBase Allele Report: Dmel\Tao[KK108458]

General Information

Symbol

Dmel\Tao^KK108458

Species

D. melanogaster

Name

FlyBase ID

FBal0235586

Feature type

allele

Associated gene

Dmel\Tao

Associated Insertion(s)

Carried in Construct

P{KK108458}

Also Known As

UAS-tao-1 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK108458}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK108458

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of central nervous system disease

CEC

(Dulovic-Mahlow et al., 2019)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Poon et al., 2016)

abnormal size | adult stage, with Scer\GAL4^dpp.blk1

(Boggiano et al., 2011)

abnormal size | adult stage, with Scer\GAL4^GMR.PF

(Poon et al., 2011)

abnormal size | adult stage | somatic clone, with Scer\GAL4^GMR.PF

(Poon et al., 2011)

abnormal size | third instar larval stage, with Scer\GAL4^ap-md544

(Boggiano et al., 2011)

abnormal size | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Poon et al., 2016)

abnormal size | third instar larval stage, with Scer\GAL4^mirr-DE

(Boggiano et al., 2011)

increased cell number | pupal stage, with Scer\GAL4^GMR.PF

(Poon et al., 2011)

increased cell number | pupal stage, with Scer\GAL4^GMR.PU

(Boggiano et al., 2011)

lethal - all die before end of P-stage, with Scer\GAL4^da.PU

(Dulovic-Mahlow et al., 2019)

majority die during third instar larval stage, with Scer\GAL4^da.PU

(Dulovic-Mahlow et al., 2019)

visible | adult stage, with Scer\GAL4^GMR.PF

(Poon et al., 2011)

Phenotype Manifest In

adult cuticle | somatic clone, with Scer\GAL4^GMR.PF

(Poon et al., 2011)

eye, with Scer\GAL4^GMR.PF

(Poon et al., 2011)

eye | somatic clone, with Scer\GAL4^GMR.PF

(Poon et al., 2011)

eye disc | dorsal | third instar larval stage, with Scer\GAL4^mirr-DE

(Boggiano et al., 2011)

head | somatic clone, with Scer\GAL4^GMR.PF

(Poon et al., 2011)

interommatidial cell | pupal stage, with Scer\GAL4^GMR.PF

(Poon et al., 2011)

interommatidial cell | pupal stage, with Scer\GAL4^GMR.PU

(Boggiano et al., 2011)

larval ventral nerve cord | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Poon et al., 2016)

wing, with Scer\GAL4^dpp.blk1

(Boggiano et al., 2011)

wing disc | third instar larval stage, with Scer\GAL4^ap-md544

(Boggiano et al., 2011)

Detailed Description

Statement

Reference

Expressing Tao^KK108458 under the control of Scer\GAL4^insc-Mz1407 (in combination with a Dicer-2 transgene to enhance RNAi efficiency) results in a significant volume increase of the ventral nerve cord at third instar larval stage.

(Poon et al., 2016)

Expression of Tao^KK108458 under the control of Scer\GAL4^ap-md544 causes massive overgrowth of third instar wing imaginal discs compared to wild type.

In adult wings from animals expressing Tao^KK108458 under the control of Scer\GAL4^dpp.blk1, extra folds and vein material are present. The area between veins three and fours shows a statistically significant increased in size compared to wild type wings.

Expression of Tao^KK108458 in the dorsal compartment of the eye using Scer\GAL4^mirr-DE results in an increase in the size of the eye imaginal discs. The dorsal compartment is grossly overgrown relative to both the ventral compartment and to wild type controls.

An increased number of interommatidial cells is seen in pupal eyes when Tao^KK108458 is expressed under the control of Scer\GAL4^GMR.PF. Unlike in wild type, the third instar larval eye disc cells continue to divide posterior to the second mitotic wave.

(Boggiano et al., 2011)

Expression of Tao^KK108458 under the control of Scer\GAL4^GMR.PF gives rise to slightly larger eyes, particularly in the posterior region of the eye.

Expression of Tao^KK108458 under the control of Scer\GAL4^GMR.PF causes an increase in the number of interommatidial cells in eyes dissected 44 hours after puparium formation.

Expression of Tao^KK108458 under the control of Scer\GAL4^GMR.PF in eye disc clones (using the eyFLP method) results in flies that have large heads compared to controls. Extra head cuticle is obvious between the eyes and the eyes themselves are convoluted.

(Poon et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Tao^KK108458/Scer\GAL4^GMR.PF is an enhancer of increased cell number | pupal stage phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.YFP}

(Poon et al., 2011)

Suppressor of

Statement

Reference

Tao^KK108458/Scer\GAL4^GMR.PF is a suppressor of visible | adult stage phenotype of hpo^GMR.PW

(Poon et al., 2011)

Tao^KK108458/Scer\GAL4^GMR.PF is a suppressor of abnormal size | adult stage phenotype of hpo^GMR.PW

(Poon et al., 2011)

NOT Suppressor of

Statement

Reference

Tao^KK108458/Scer\GAL4^GMR.PF is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PF, wts^UAS.Tag:MYC

(Poon et al., 2011)

Phenotype Manifest In

Enhancer of

Statement

Reference

Tao^KK108458/Scer\GAL4^GMR.PF is an enhancer of interommatidial cell | pupal stage phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.YFP}

(Poon et al., 2011)

Suppressor of

Statement

Reference

Tao^KK108458/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of hpo^GMR.PW

(Poon et al., 2011)

NOT Suppressor of

Statement

Reference

Tao^KK108458/Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, wts^UAS.Tag:MYC

(Poon et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Tao^KK108458 enhances the increase in inter-ommatidial cell number seen when yki^{S168A.Scer\UAS.T:Avic\GFP-YFP} is expressed under the control of Scer\GAL4^GMR.PF.

Expression of Tao^KK108458 has no effect on the reduction in eye size seen when wts^{Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^GMR.PF.

Expression of Tao^KK108458 under the control of Scer\GAL4^GMR.PF almost completely suppresses the reduced eye size seen when hpo^GMR.PW is expressed.

(Poon et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Tao^KK108458 is rescued by Scer\GAL4^GMR.PF/Tao^EP1455

(Poon et al., 2011)

Comments

Expression of Tao^EP1455 rescues the eye overgrowth phenotype seen when Tao^KK108458 is expressed under the control of Scer\GAL4^GMR.PF.

(Poon et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v107645

P{KK108458}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Tao-1^KK108458

Tao^KK108458

Tao^VDRC

(Chen et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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