FlyBase Allele Report: Dmel\yki[S168A.UAS.YFP]

General Information

Symbol

Dmel\yki^{S168A.UAS.YFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0265566

Feature type

allele

Associated gene

Dmel\yki

Associated Insertion(s)

Carried in Construct

P{UAS-yki.S168A.YFP}

Key Links

Transgenic product class

missense_variant

(Zhang et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Zhang et al., 2009)

Mutagen

in vitro construct

(Zhang et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-yki.S168A.YFP}

Cytology

Description

UAS regulatory sequences drive expression of a mutant form of yki in which the serine at position 168 has been replaced by an alanine. The construct is tagged with YFP.

(Zhang et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

yki

Tagged with

YFP

yellow fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | larval stage, with Scer\GAL4^GMR.PU

(Zhang et al., 2015)

abnormal size, with Scer\GAL4^GMR.PF

(Zhang et al., 2009)

abnormal size | adult stage, with Scer\GAL4^GMR.PU

(Zhang et al., 2015)

abnormal size | larval stage, with Scer\GAL4^GMR.PU

(Zhang et al., 2015)

increased cell number | pupal stage, with Scer\GAL4^GMR.PF

(Poon et al., 2011)

increased occurrence of cell division | larval stage, with Scer\GAL4^GMR.PU

(Zhang et al., 2015)

visible, with Scer\GAL4^GMR.PF

(Dent et al., 2015, Zhang et al., 2009)

visible, with Scer\GAL4^GMR.PU

(Zhang et al., 2015)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Dent et al., 2015, Zhang et al., 2009)

eye, with Scer\GAL4^GMR.PU

(Zhang et al., 2015)

eye disc | larval stage, with Scer\GAL4^GMR.PU

(Zhang et al., 2015)

eye disc posterior to the morphogenetic furrow | larval stage, with Scer\GAL4^GMR.PU

(Zhang et al., 2015)

interommatidial cell | pupal stage, with Scer\GAL4^GMR.PF

(Poon et al., 2011)

Detailed Description

Statement

Reference

Flies expressing yki^{S168A.Scer\UAS.T:Avic\GFP-YFP} under the control of Scer\GAL4^GMR.PF have a subtle eye overgrowth phenotype.

(Dent et al., 2015)

Expression of yki^{S168A.Scer\UAS.T:Avic\GFP-YFP} under the control of Scer\GAL4^GMR.PU leads to overgrowth of the adult eye, larval eye discs show precocious entry into the S phase of the cell cycle, and enlargement of the sheet of epithelial tissue posterior to the morphogenetic furrow.

(Zhang et al., 2015)

Expression of yki^{S168A.Scer\UAS.T:Avic\GFP-YFP} under the control of Scer\GAL4^GMR.PF causes an increase in the number of interommatidial cells in eyes dissected 44 hours after puparium formation.

(Poon et al., 2011)

Expression of yki^{S168A.Scer\UAS.T:Avic\GFP-YFP} under the control of Scer\GAL4^GMR.PF results in overgrowth of the adult eye.

(Zhang et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, yki^{S168A.UAS.YFP} has visible phenotype, enhanceable by RtGEF^HMS00741, Scer\GAL4^GMR.PF

(Dent et al., 2015)

Scer\GAL4^GMR.PF, yki^{S168A.UAS.YFP} has increased cell number | pupal stage phenotype, enhanceable by Tao^KK108458/Scer\GAL4^GMR.PF

(Poon et al., 2011)

Suppressed by

Statement

Reference

RtGEF^HMS00741, Scer\GAL4^GMR.PF, yki^{S168A.UAS.YFP} has visible phenotype, suppressible by sd^KK108877, Scer\GAL4^GMR.PF

(Dent et al., 2015)

Scer\GAL4^GMR.PU, yki^{S168A.UAS.YFP} has abnormal size | adult stage phenotype, suppressible by sd^RNAi.UAS.cUa/Scer\GAL4^GMR.PU

(Zhang et al., 2015)

Scer\GAL4^GMR.PU, yki^{S168A.UAS.YFP} has visible phenotype, suppressible by sd^RNAi.UAS.cUa/Scer\GAL4^GMR.PU

(Zhang et al., 2015)

Scer\GAL4^GMR.PU, yki^{S168A.UAS.YFP} has abnormal size | adult stage phenotype, suppressible by nanos^JF02931/Scer\GAL4^GMR.PU

(Zhang et al., 2015)

Scer\GAL4^GMR.PU, yki^{S168A.UAS.YFP} has visible phenotype, suppressible by nanos^JF02931/Scer\GAL4^GMR.PU

(Zhang et al., 2015)

Scer\GAL4^GMR.PU, yki^{S168A.UAS.YFP} has abnormal size | adult stage phenotype, suppressible by piwi^HMS00185/Scer\GAL4^GMR.PU

(Zhang et al., 2015)

Scer\GAL4^GMR.PU, yki^{S168A.UAS.YFP} has visible phenotype, suppressible by piwi^HMS00185/Scer\GAL4^GMR.PU

(Zhang et al., 2015)

Scer\GAL4^GMR.PU, yki^{S168A.UAS.YFP} has abnormal size | adult stage phenotype, suppressible by piwi^HMS00606/Scer\GAL4^GMR.PU

(Zhang et al., 2015)

Scer\GAL4^GMR.PU, yki^{S168A.UAS.YFP} has visible phenotype, suppressible by piwi^HMS00606/Scer\GAL4^GMR.PU

(Zhang et al., 2015)

Other

Statement

Reference

Scer\GAL4^GMR.PU, tai^HM05182, yki^{S168A.UAS.YFP} has abnormal size | adult stage phenotype

(Zhang et al., 2015)

Scer\GAL4^GMR.PU, tai^HM05182, yki^{S168A.UAS.YFP} has partially lethal phenotype

(Zhang et al., 2015)

Scer\GAL4^GMR.PU, tai^61G1, yki^{S168A.UAS.YFP} has abnormal size | adult stage phenotype

(Zhang et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, yki^{S168A.UAS.YFP} has eye phenotype, enhanceable by RtGEF^HMS00741, Scer\GAL4^GMR.PF

(Dent et al., 2015)

Scer\GAL4^GMR.PF, yki^{S168A.UAS.YFP} has interommatidial cell | pupal stage phenotype, enhanceable by Tao^KK108458/Scer\GAL4^GMR.PF

(Poon et al., 2011)

Suppressed by

Statement

Reference

RtGEF^HMS00741, Scer\GAL4^GMR.PF, yki^{S168A.UAS.YFP} has eye phenotype, suppressible by sd^KK108877, Scer\GAL4^GMR.PF

(Dent et al., 2015)

Scer\GAL4^GMR.PU, yki^{S168A.UAS.YFP} has eye phenotype, suppressible by tai^HM05182/Scer\GAL4^GMR.PU

(Zhang et al., 2015)

Scer\GAL4^GMR.PU, yki^{S168A.UAS.YFP} has eye phenotype, suppressible by tai^61G1/tai^61G1

(Zhang et al., 2015)

Scer\GAL4^GMR.PU, yki^{S168A.UAS.YFP} has eye phenotype, suppressible by sd^RNAi.UAS.cUa/Scer\GAL4^GMR.PU

(Zhang et al., 2015)

Scer\GAL4^GMR.PU, yki^{S168A.UAS.YFP} has eye phenotype, suppressible by nanos^JF02931/Scer\GAL4^GMR.PU

(Zhang et al., 2015)

Scer\GAL4^GMR.PU, yki^{S168A.UAS.YFP} has eye phenotype, suppressible by piwi^HMS00185/Scer\GAL4^GMR.PU

(Zhang et al., 2015)

Scer\GAL4^GMR.PU, yki^{S168A.UAS.YFP} has eye phenotype, suppressible by piwi^HMS00606/Scer\GAL4^GMR.PU

(Zhang et al., 2015)

Other

Statement

Reference

Scer\GAL4^GMR.PU, tai^HM05182, yki^{S168A.UAS.YFP} has adult head phenotype

(Zhang et al., 2015)

Scer\GAL4^GMR.PU, tai^61G1, yki^{S168A.UAS.YFP} has adult head phenotype

(Zhang et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of RtGEF^HMS00741 enhances the eye overgrowth phenotype caused by expression of yki^{S168A.Scer\UAS.T:Avic\GFP-YFP} under the control of Scer\GAL4^GMR.PF. There is an increase in eye size with folding and convolution of the eye.

sd^KK108877 completely suppresses the eye overgrowth phenotype seen in flies co-expressing RtGEF^HMS00741 and yki^{S168A.Scer\UAS.T:Avic\GFP-YFP} under the control of Scer\GAL4^GMR.PF.

(Dent et al., 2015)

Co-expression of tai^Scer\UAS.cBa enhances eye overgrowth in adults, and in larval eye discs enhances early S-phase entry, leads to greater spacing between apical tufts of photoreceptor clusters, and leads to large folds of excess tissue posterior to the morphogenetic furrow, in flies expressing yki^{S168A.Scer\UAS.T:Avic\GFP-YFP} under the control of Scer\GAL4^GMR.PU.

Co-expression of tai^HM05182 leads to low adult survival, but shrinks both adult head and eye size in flies expressing yki^{S168A.Scer\UAS.T:Avic\GFP-YFP} under the control of Scer\GAL4^GMR.PU.

tai^61G1/tai^61G1 clones lead to reduced head and eye size in flies expressing yki^{S168A.Scer\UAS.T:Avic\GFP-YFP} under the control of Scer\GAL4^GMR.PU.

Co-expression of sd^{dsRNA.Scer\UAS.cUa}, nos^JF02931, piwi^HMS00185 or piwi^HMS00606 rescues the eye overgrowth seen in adults expressing yki^{S168A.Scer\UAS.T:Avic\GFP-YFP} under the control of Scer\GAL4^GMR.PU.

Co-expression of tai^{PPxA1-2.Scer\UAS} partially rescues the eye overgrowth seen in adults, and in larval eye discs suppresses the early S-phase entry seen in flies expressing yki^{S168A.Scer\UAS.T:Avic\GFP-YFP} under the control of Scer\GAL4^GMR.PU.

(Zhang et al., 2015)

Expression of Tao^KK108458 enhances the increase in inter-ommatidial cell number seen when yki^{S168A.Scer\UAS.T:Avic\GFP-YFP} is expressed under the control of Scer\GAL4^GMR.PF.

(Poon et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

yki^{S168A.Scer\UAS.T:Avic\GFP-YFP}

yki^{S168A.UAS.YFP}

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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